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In-vitro model to mimic T cell subset change in human PDAC organoid co-culture
PurposeImmunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational...
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Investigation of the optimal light parameters for photobiomodulation to induce osteogenic differentiation of the human bone marrow stem cell and human umbilical vein endothelial cell co-culture
Bones have an important role in the human body with their complex nature. Mesenchymal stem cells and endothelial cells together support their unique...
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In vitro co-culture studies and the crucial role of fibroblast-immune cell crosstalk in IPF pathogenesis
IPF is a fatal lung disease characterized by intensive remodeling of lung tissue leading to respiratory failure. The remodeling in IPF lungs is...
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Evaluating cell culture reliability in pediatric brain tumor primary cells through DNA methylation profiling
In vitro models of pediatric brain tumors (pBT) are instrumental for better understanding the mechanisms contributing to oncogenesis and testing new...
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Electrophysiological and calcium-handling development during long-term culture of human-induced pluripotent stem cell-derived cardiomyocytes
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are increasingly used for personalised medicine and preclinical cardiotoxicity...
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The origin and possible mechanism of embryonic cell-free DNA release in spent embryo culture media: a review
The presence of cell-free DNA in spent embryo culture media (SECM) has unveiled its possible utilization for embryonic ploidy determination, opening...
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Modified expi293 cell culture system using piggyBac transposon enables efficient production of human FVIII
Human blood coagulation factor VIII (hFVIII) is used in hemostatic and prophylactic treatment of patients with hemophilia A. Biotechnological...
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Prolonged culture of human pancreatic islets under glucotoxic conditions changes their acute beta cell calcium and insulin secretion glucose response curves from sigmoid to bell-shaped
Aims/hypothesisThe rapid remission of type 2 diabetes by a diet very low in energy correlates with a marked improvement in glucose-stimulated insulin...
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Three-Dimensional Cell Culture Models of Hepatocellular Carcinoma — a Review
IntroductionThree-dimensional (3D) cell culture studies are becoming extremely common because of their capability to mimic tumor architecture, such...
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Altered expression of proteins involved in metabolism in LGMDR1 muscle is lost in cell culture conditions
BackgroundLimb-girdle muscular dystrophy R1 calpain 3-related (LGMDR1) is an autosomal recessive muscular dystrophy due to mutations in the CAPN3 ...
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A morphological study of adipose-derived stem cell sheets created with temperature-responsive culture dishes using scanning electron microscopy
Adipose-derived stem cell (ADSC) sheets have potential to be effective in various therapies. In this study, we first demonstrated that a cell sheet...
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Bei Mu Gua Lou San facilitates mucus expectoration by increasing surface area and hydration levels of airway mucus in an air-liquid-interface cell culture model of the respiratory epithelium
BackgroundBei Mu Gua Lou San (BMGLS) is an ancient formulation known for its moisturizing and expectorant properties, but the underlying mechanisms...
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Predictors of culture-negative peritoneal dialysis-associated peritonitis: a single center, retrospective study
BackgroundEmpirical antibiotic treatment against peritoneal dialysis (PD)-related peritonitis should be immediately initiated before PD effluent...
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Efficacy and safety of two-stage revision for patients with culture-negative versus culture-positive periprosthetic joint infection: a single-center retrospective study
BackgroundThe safety and efficacy of two-stage revision for culture-negative PJI remain controversial. This study analyzed outcomes after two-stage...
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Microfluidic Co-culture Platforms for Studying Osteocyte Regulation of Other Cell Types under Dynamic Mechanical Stimulation
Purpose of ReviewOsteocytes are the most abundant cell type in bone. These unique cells act primarily as mechanosensors and play crucial roles in the...
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Culture expansion of CAR T cells results in aberrant DNA methylation that is associated with adverse clinical outcome
Chimeric antigen receptor (CAR) T cells provide new perspectives for treatment of hematological malignancies. Manufacturing of these cellular...
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Concordance of bone culture and deep tissue culture during the operation of diabetic foot osteomyelitis and clinical characteristics of patients
PurposeTo retrospectively analyze the concordance of bacterial culture between bone tissue and deep soft tissue in diabetic foot osteomyelitis (DFO)...
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LINC01119 encapsulated by cancer-associated adipocytes-derived exosomes promotes M2 polarization of macrophages to induce immune escape in ovarian cancer in a 3D co-culture cell-based model
IntroductionIn the present study, we sought to clarify the role of LINC01119 delivered by cancer-associated adipocytes (CAAs)-derived exosomes...
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Activity of trastuzumab emtansine (T-DM1) in 3D cell culture
BackgroundCell spheroids and aggregates generated from three-dimensional (3D) cell culture methods are similar to in vivo tumors in terms of tissue...
