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Preparation of a highly specific and sensitive monoclonal antibody against tilmicosin and its application in lateral flow immunoassay
To reduce the false positive results caused by cross reactivity of the antibodies with other structural analogues, it is crucial to prepare a high...
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Novel triplex nucleic acid lateral flow immunoassay for rapid detection of Nipah virus, Middle East respiratory syndrome coronavirus and Reston ebolavirus
We report the development of a triplex nucleic acid lateral flow immunoassay (NALFIA) for the detection of the genomes of Nipah virus (NiV), Middle...
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Development of a chromatographic lateral flow immunoassay for detection of African swine fever virus antigen in blood
African swine fever (ASF) is a highly lethal disease of domestic and wild swine caused by African swine fever virus (ASFV). The disease currently...
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Machine learning to support visual auditing of home-based lateral flow immunoassay self-test results for SARS-CoV-2 antibodies
BackgroundLateral flow immunoassays (LFIAs) are being used worldwide for COVID-19 mass testing and antibody prevalence studies. Relatively simple to...
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Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design
Porcine epidemic diarrhea virus (PEDV), as the main causative pathogen of viral diarrhea in pigs, has been reported to result in high morbidity and...
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Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury
BackgroundAKI is related to severe adverse outcomes and mortality with Coronavirus Disease 2019 (COVID-19) patients, that early diagnosed and...
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Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population
BackgroundRapid determination of an individual’s antibody status can be beneficial in understanding an individual’s immune response to SARS-CoV-2 and...
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An urgent need for community lot testing of lateral flow fentanyl test strips marketed for harm reduction in Northern America
BackgroundFentanyl test strips (FTS) are lateral flow immunoassay strips designed for detection of ng/mL levels of fentanyl in urine. In 2021, the US...
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Development of a lateral flow immunochromatography assay for the detection of fluoroacetamide in blood samples
Fluoroacetamide (FAM) has been employed as a rodenticide for an extended duration, leading to a multitude of incidents involving human ingestion...
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Lateral flow assay (LFA) in the diagnosis of COVID-19-associated pulmonary aspergillosis (CAPA): a single-center experience
BackgroundInvasive pulmonary aspergillosis (IPA) is seen during coronavirus-2019 (COVID-19), has been reported in different incidences, and is...
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Development and clinical application of loop-mediated isothermal amplification combined with lateral flow assay for rapid diagnosis of SARS-CoV-2
BackgroundThe diagnostic assay leveraging multiple reverse transcription loop-mediated isothermal amplification (RT-LAMP) could meet the requirements...
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An automated fluorescent immunoassay for on-site screening of AFM1 in raw milk at the ppt level
In the dairy industry, the quality of raw milk as it is collected from dairy farmers must be strictly ensured. Therefore, when on-site screening...
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Evaluation of qualitative and semi-quantitative cut offs for rapid diagnostic lateral flow test in relation to serology for the detection of SARS-CoV-2 antibodies: findings of a prospective study
Background:There is limited information to compare the qualitative and semi-quantitative performance of rapid diagnostic tests (RDT) and serology for...
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Multicenter Evaluation of a Fully Automated High-Throughput SARS-CoV-2 Antigen Immunoassay
IntroductionMolecular testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to suffer from delays and shortages. Antigen...
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Cerebrospinal fluid and blood exosomes as biomarkers for amyotrophic lateral sclerosis; a systematic review
BackgroundAmyotrophic lateral sclerosis (ALS) is a progressive and fatal motor neuron disease. Due to the limited knowledge about potential...
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Hapten design to prepare monoclonal antibodies and establishment of immunoassay for direct screening of oxamichydrazide in chicken, the metabolite of nifuraldezone
In this study, two novel haptens of oxamichydrazide (OXZ), the metabolite of nifuraldezone, were designed and synthesized by derivatization with...
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Evaluation of serological lateral flow assays for severe acute respiratory syndrome coronavirus-2
BackgroundCOVID-19 has resulted in significant morbidity and mortality worldwide. Lateral flow assays can detect anti-Severe Acute Respiratory...
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Clinical Application of Antibody Immunity Against SARS-CoV-2: Comprehensive Review on Immunoassay and Immunotherapy
The current COVID-19 global pandemic poses immense challenges to global health, largely due to the difficulty to detect infection in the early stages...
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Diagnostic performance of the IMMY cryptococcal antigen lateral flow assay on serum and cerebrospinal fluid for diagnosis of cryptococcosis in HIV-negative patients: a systematic review
BackgroundThe incidence of cryptococcosis amongst HIV-negative persons is increasing. Whilst the excellent performance of the CrAg testing in people...
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Performance of antigen testing for diagnosis of COVID-19: a direct comparison of a lateral flow device to nucleic acid amplification based tests
ObjectivesThe gold standard for diagnosing an infection with SARS-CoV-2 is detection of viral RNA by nucleic acid amplification techniques. Test...
