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The Impact of Centrifugal Force on Isolation of Bone Marrow Mononuclear Cells Using Density Gradient Centrifugation
BackgroundBone marrow mononuclear cells (BMMNCs) have great potential in bone regenerative therapy. The main method used today to obtain BMMNCs is...
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Sperm Preparation with Microfluidic Sperm Sorting Chip May Improve Intracytoplasmic Sperm Injection Outcomes Compared to Density Gradient Centrifugation
Does sperm preparation using the FERTILE PLUS™ Sperm Sorting Chip improve fertilization rates, blastocyst formation, utilization, and euploidy rates...
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Efficacy of density gradient centrifugation technique (DGC) in enhancing sperm cell DNA quality for assisted reproductive technique
ObjectiveDensity gradient centrifugation (DGC) is commonly used for sperm preparation before assisted reproductive technology (ART) procedures. This...
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Live motile sperm sorting device for enhanced sperm-fertilization competency: comparative analysis with density-gradient centrifugation and microfluidic sperm sorting
PurposeA live motile sperm sorting device (LensHooke ® CA0) developed to prevent the deleterious effects of centrifugation was evaluated...
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A comparison between the Felix™ electrophoretic system of sperm isolation and conventional density gradient centrifugation: a multicentre analysis
PurposeDevelo** optimized techniques for the isolation of human spermatozoa possessing low levels of DNA damage is an important objective for the...
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The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa
BackgroundThe wisent ( Bison bonasus ) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and...
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Embryologic outcomes among patients using a microfluidics chip compared to density gradient centrifugation to process sperm: a paired analysis
PurposeTo evaluate embryologic outcomes among paired IVF cycles in which a microfluidics chip was utilized compared to density gradient...
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Cumulative live birth rates after IVF/ICSI cycles with sperm prepared by density gradient centrifugation vs. swim-up: a retrospective study using a propensity score-matching analysis
BackgroundDensity gradient centrifugation (DGC) and swim-up (SU) are the two most widely used sperm preparation methods for in vitro fertilization...
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The density of bone marrow mononuclear cells and CD34+ cells in patients with three neurologic conditions
BackgroundThis study aimed to identify the density of mononuclear cells (MNCs) and CD34+ cells in the bone marrow of patients with three neurologic...
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A Simple, Centrifugation-Free, Sperm-Sorting Device Eliminates the Risks of Centrifugation in the Swim-Up Method While Maintaining Functional Competence and DNA Integrity of Selected Spermatozoa
This pilot study was conducted to explore the benefits of using a centrifugation-free device based on the migration–sedimentation (MS) technique over...
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Density-Based High-Quality Fat: Characterization and Correlation with Different Body Fat Ratio
BackgroundLipoaspirate can be divided into high-quality fat and low-quality fat using Coleman’s centrifugation by adding 0.935 g/ml marker float; the...
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DNA fragmentation in concert with the simultaneous assessment of cell viability in a subfertile population: establishing thresholds of normality both before and after density gradient centrifugation
PurposeTUNEL assay is the most common, direct test for sperm chromatin integrity assessment. But, lack of standardized protocols makes...
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An integrated microfluidics platform with high-throughput single-cell cloning array and concentration gradient generator for efficient cancer drug effect screening
BackgroundTumor cell heterogeneity mediated drug resistance has been recognized as the stumbling block of cancer treatment. Elucidating the...
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Emerging trends in sperm selection: enhancing success rates in assisted reproduction
This comprehensive review explores the evolving landscape of sperm selection techniques within the realm of Assisted Reproductive Technology (ART)....
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Optimized sperm selection: a highly efficient device for the isolation of progressive motile sperm with low DNA fragmentation index
PurposeTo identify the sperm preparation procedure that selects the best sperm population for medically assisted reproduction.
MethodsProspective...
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Optimization of a rapid one-step platelet-rich plasma preparation method using syringe centrifugation with and without carprofen
BackgroundCarprofen and platelet-rich plasma (PRP) are widely used in small animal clinical practice. Separation layers have been used during blood...
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High density lipoprotein inhibited group II innate lymphoid cells proliferation and function in allergic rhinitis
BackgroundMore and more studies had suggested that dyslipidemia was closely related to allergic diseases. High density lipoprotein (HDL) often plays...
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Effect of Sperm 26S Proteasome on Embryo Formation and Development in In Vitro Fertilization
The aim of this study was to evaluate the effects of sperm proteasome activity on fertilization outcome and embryo development after IVF. Following...
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Microfluidic chips as a method for sperm selection improve fertilization rate in couples with fertilization failure
PurposeSperm quality plays a vital role in successful fertilization and pregnancy. Patients with fertilization failure (total failure or...
