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Tandem Mass Tag-Based Phosphoproteomics in Plants
Mass spectrometry-based proteomics provide a powerful tool for plant research, allowing global detection of steady-state levels of proteins under a...
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Proteomic analysis of plasma proteins from patients with cardiac rupture after acute myocardial infarction using TMT-based quantitative proteomics approach
BackgroundCardiac rupture (CR) is a rare but catastrophic mechanical complication of acute myocardial infarction (AMI) that seriously threatens human...
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Assessing the Phagosome Proteome by Quantitative Mass Spectrometry
The process of phagocytosis involves a series of defined steps, including the formation of a new intracellular organelle, i.e., the phagosome, and...
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TMT-based quantitative proteomics analysis of the effects of Jiawei Danshen decoction myocardial ischemia-reperfusion injury
BackgroundEvery year, approximately 17 million people worldwide die due to coronary heart disease, with China ranking second in terms of the death...
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Optimized sample preparation and data analysis for TMT proteomic analysis of cerebrospinal fluid applied to the identification of Alzheimer’s disease biomarkers
BackgroundCerebrospinal fluid (CSF) is an important biofluid for biomarkers of neurodegenerative diseases such as Alzheimer’s disease (AD). By...
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TMT proteomic analysis for molecular mechanism of Staphylococcus aureus in response to freezing stress
AbstractThe foodborne pathogen Staphylococcus aureus continues to challenge the food industry due to the pathogenicity and tolerance of the...
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TMT-based quantitative proteomic profiling of human monocyte-derived macrophages and foam cells
BackgroundCardiovascular diseases remain the leading cause of morbidity and mortality worldwide, most of which are caused by atherosclerosis....
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TMT-based quantitative proteomic analysis of spheroid cells of endometrial cancer possessing cancer stem cell properties
BackgroundCancer stem cells (CSCs) play an important role in endometrial cancer progression and it is potential to isolate CSCs from spheroid cells....
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Tandem mass tag-based (TMT) quantitative proteomics analysis reveals the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)
BackgroundCotton ( Gossypium hirsutum L.) is one of the most important cash crops worldwide. Fine roots are the central part of the root system that...
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A streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics
Effective phosphoproteome of nanoscale sample analysis remains a daunting task, primarily due to significant sample loss associated with non-specific...
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Biomarkers for subclinical bovine mastitis: a high throughput TMT-based proteomic investigation
Mastitis represents the biggest threat to the health and productivity of dairy cows, leading to substantial economic losses in milk production. It...
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High-Throughput Profiling of Proteome and Posttranslational Modifications by 16-Plex TMT Labeling and Mass Spectrometry
Mass spectrometry (MS)-based proteomic profiling of whole proteome and protein posttranslational modifications (PTMs) is a powerful technology to...
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TMT-based proteomic analysis of liquorice root in response to drought stress
BackgroundDrought stress is a serious threat to land use efficiency and crop yields worldwide. Understanding the mechanisms that plants use to...
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Mass spectrometry-based proteomic landscape of rice reveals a post-transcriptional regulatory role of N6-methyladenosine
Rice is one of the most important staple food and model species in plant biology, yet its quantitative proteomes are largely uncharacterized. Here we...
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Comparative Proteomics Analysis Reveals the Molecular Mechanisms Underlying the Accumulation Difference of Bioactive Constituents in Taxilli Herba from Two Hosts
Taxilli Herba (TH) is famous for semi-parasitic feature, with hundreds of host species. The content of bioactive constituents varies in TH from...
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TMT-based quantitative proteomics analysis reveals the key proteins related with the differentiation process of goat intramuscular adipocytes
BackgroundIntramuscular adipocytes differentiation is a complex process, which is regulated by various transcription factor, protein factor...
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Proteomic analysis of the responses to chelerythrine in the fungal pathogen Ustilaginoidea virens
Chelerythrine (CHE) is a natural benzophenanthridine alkaloid with broad pharmacological activities. Herein, we investigated the responses to CHE...
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Quantitative proteomics analysis reveals the key proteins related to semen quality in Niangya yaks
BackgroundProteins related to sperm motility and sperm morphology have an important impact on sperm function such as metabolism, motility and...
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Quantitative Translation Proteomics Using mePROD
Multiplexed enhanced protein dynamic mass spectrometry (mePROD MS) enables robust quantification of translation in cell culture. Tandem mass tags...
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Proteome Based Comparative Investigation of a High Sucrose Sugarcane Mutant in Contrast to the Low Sucrose Mother Variety by Using TMT Quantitative Proteomics
Sugarcane ( Saccharum sp. hybrid) is a significant crop in the world’s economy and fulfills 80% of the world's sugar production. To meet the rising...
