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Recombinant Chymotrypsin-like Peptidase from Tenebrio molitor with a Non-Canonical Substrate-Binding Site
AbstractWe characterized an alkaline chymotrypsin-like serine peptidase from the yellow mealworm Te-nebrio molitor with a non-canonical...
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Substrate binding and inhibition of the anion exchanger 1 transporter
Anion exchanger 1 (AE1), a member of the solute carrier (SLC) family, is the primary bicarbonate transporter in erythrocytes, regulating pH levels...
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Investigating the effect of substrate binding on the catalytic activity of xylanase
AbstractXynAF1 from Aspergillus fumigatus Z5 is an efficient thermophilic xylanase belonging to glycoside hydrolase family 10 (GH10). The...
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Optimizing the binding of OGT and a peptidic substrate towards pseudo-substrate inhibitors via molecular dynamic simulations
Elevated O -GlcNAcylation has been shown to be closely correlated with the occurrence and development of cancer, and inhibiting O -GlcNAc transferase...
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The substrate and inhibitor binding mechanism of polyspecific transporter OAT1 revealed by high-resolution cryo-EM
Organic anion transporters (OATs) of the SLC22 family have crucial roles in the transport of organic anions, including metabolites and therapeutic...
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Structural perturbations of substrate binding and oxidation state changes in a lytic polysaccharide monooxygenase
LPMOs are enzymes which catalyse the oxidation of a C-H bond within polysaccharides, leading to their oxidative cleavage. To achieve this, LPMOs...
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Polymorphism on human aromatase affects protein dynamics and substrate binding: spectroscopic evidence
Human aromatase is a member of the cytochrome P450 superfamily, involved in steroid hormones biosynthesis. In particular, it converts androgen into...
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Bioinformatic Analysis of Substrate Binding Sites in Decapod Brachyurin-C Collagenases
AbstractBrachyurin-C is the only member of the chymotrypsin A family able to hydrolyze native collagen type I and it is found in decapods like crabs,...
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Cyanide replaces substrate in obligate-ordered addition of nitric oxide to the non-heme mononuclear iron AvMDO active site
Thiol dioxygenases are a subset of non-heme mononuclear iron oxygenases that catalyze the O 2 -dependent oxidation of thiol-bearing substrates to yield...
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High-resolution structure of the amino acid transporter AdiC reveals insights into the role of water molecules and networks in oligomerization and substrate binding
BackgroundThe L-arginine/agmatine transporter AdiC is part of the arginine-dependent extreme acid resistance system of the bacterium Escherichia coli ...
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Crystallographic Studies of Native and Mutant Orotidine 5′phosphate Decarboxylases
This review aims to use the results of an approach combining crystallographic structure analysis with mutational studies as a framework for the... -
Site-Specified Conjugating Technology and Application
Conjugation strategy is a very important consideration when designing antibody-drug conjugates (ADCs). Site-specified conjugation is the conjugation... -
Substrate-binding destabilizes the hydrophobic cluster to relieve the autoinhibition of bacterial ubiquitin ligase IpaH9.8
IpaH enzymes are bacterial E3 ligases targeting host proteins for ubiquitylation. Two autoinhibition modes of IpaH enzymes have been proposed based...
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O-Acetylhomoserine Sulfhydrylase from Clostridioides difficile: Role of Tyrosine Residues in the Active Site
AbstractO -acetylhomoserine sulfhydrylase is one of the key enzymes in biosynthesis of methionine in Clostridioides difficile . The mechanism of...
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Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases
Hyperthermophilic (‘superheat-loving’) archaea found in high-temperature environments such as Pyrobaculum aerophilum contain multicopper oxidases...
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A carboxy-terminal ubiquitylation site regulates androgen receptor activity
Degradation of unliganded androgen receptor (AR) in prostate cancer cells can be prevented by proteasome inhibition, but this is associated with only...
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Defective internal allosteric network imparts dysfunctional ATP/substrate-binding cooperativity in oncogenic chimera of protein kinase A
An aberrant fusion of the DNAJB1 and PRKACA genes generates a chimeric protein kinase (PKA-C DNAJB1 ) in which the J-domain of the heat shock protein...
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Insight into the Catalytic Mechanism of Orotidine 5′-phosphate Decarboxylase from Crystallography and Mutagenesis
With a half-time of 78 million years at room temperature, the spontaneous decarboxylation of orotidine 5′-phosphate (OMP) is among the slowest... -
Antibody and antibody fragments site-specific conjugation using new Q-tag substrate of bacterial transglutaminase
During the last few years Antibody-Drug Conjugates (ADCs) have become one of the most active and very promising therapeutic weapons. Lessons learned...