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Choice of Ribonucleases for Ribosome Profiling Experiments
In the past 10 years, standard transcriptome sequencing protocols were optimized so well that no prior experience is required to prepare the...
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The Role of Ribonucleases in RNA Damage, Inactivation and Degradation
Nucleic acids are universally present in all forms of life on earth. Since their discovery more than 150 years ago, knowledge on the roles of nucleic...
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Post-anthesis Roots Metabolic Activities Relate Low Phosphorus (P)-Tolerance in Rice (Oryza sativa L.)
Post-anthesis effect of phosphorus (P)-deficit is less studied in plants. P-deficit analysis is rarely performed in different tissues of the plant...
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Ribonucleases for Sequencing and Characterization of RNA by LC–MS
The global deployment of mRNA vaccines against SARS-CoV-2 and the projected expansion of therapeutic applications of synthetic mRNA call for robust...
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Bacterial Small RNAs: Diversity of Structure and Function
Small non-coding RNAs (sRNAs) are key post-transcriptional regulators of gene expression in bacteria, with a diversity of origins, sequences,...
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Trans-acting regulators of ribonuclease activity
RNA metabolism needs to be tightly regulated in response to changes in cellular physiology. Ribonucleases (RNases) play an essential role in almost...
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Effect of Zn2+ Ions on Acid Nuclease Activity in Freshwater Mollusks
AbstractThe effect of increased concentrations of Zn 2+ ions on the activity of the complex of acid deoxyribonucleases (DNases) and ribonucleases...
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The ribonuclease PNPase is a key regulator of biofilm formation in Listeria monocytogenes and affects invasion of host cells
Biofilms provide an environment that protects microorganisms from external stresses such as nutrient deprivation, antibiotic treatments, and immune...
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Enzymes in Molecular Biotechnology
Molecular biotechnology uses a wide variety of enzymes. These can be grouped either to their reaction mechanism (enzyme class) or to the intended use...
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The value of urinary exosomal lncRNA SNHG16 as a diagnostic biomarker for bladder cancer
ObjectiveTo detect the expression level of urinary exosomal lncRNA SNHG16 in patients with bladder cancer and healthy individuals and explore its...
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Analysis of Bacillus subtilis Ribonuclease Activity In Vivo
Ribonucleases remodel RNAs to render them functional or to send them on their way toward degradation. In our laboratory, we study these pathways in...
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RNA Damage and Repair
Ribonucleic acid (RNA) is a macromolecule that plays a central role in cell physiology: RNA molecules act as intermediates between the...
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In Vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold
Preparative synthesis of RNA is a challenging task that is usually accomplished by either chemical or enzymatic polymerization of ribonucleotides in...
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Modifications of Ribosome Profiling that Provide New Data on the Translation Regulation
AbstractRibosome profiling (riboseq) has opened the possibilities for the genome-wide studies of translation in all living organisms. This method is...
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Assay of Bacillus subtilis Ribonuclease Activity In Vitro
Ribonucleases can cleave RNAs internally in endoribonucleolytic mode or remove one nucleotide at a time from either the 5′ or 3′ end through...
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DEAD Box RNA Helicases: Biochemical Properties, Role in RNA Processing and Ribosome Biogenesis
DEAD box RNA helicases are a versatile group of ATP dependent enzymes that play an essential role in cellular processes like transcription, RNA...
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Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant
Salmonella enterica serovar Typhimurium ( S . Typhimurium) is a globally recognized foodborne pathogen that affects both animals and humans....
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Transfer RNA Fragments, from Structure to Function
Transfer RNA molecules (tRNAs) are produced from numerous nuclear and mitochondrial genes and are primarily involved in bringing specific amino acid...
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