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Efficient transformation of the isolated microspores of Chinese cabbage (Brassica rapa L. ssp. pekinensis) by particle bombardment
BackgroundThe low efficiency of genetic transformation in Chinese cabbage ( Brassica rapa L . ssp. pekinensis ) is the key problem affecting functional...
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Quantification of Cell-to-Cell Connectivity Using Particle Bombardment
Plant cells are connected by cytoplasmic bridges called plasmodesmata. Plasmodesmata are lined by the plasma membrane, essentially forming tunnels...
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Particle bombardment technology and its applications in plants
Particle bombardment, or biolistics, has emerged as an excellent alternative approach for plant genetic transformation which circumvents the...
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Establishment of a stable particle bombardment transformation method in Korean commercial wheat (Triticum aestivum L.) varieties
Wheat ( Triticum aestivum L.) is a major food crop worldwide whose complex and very large genome hinders stable genetic transformation. An optimized...
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Investigating Plasmodesmata Function in Arabidopsis Thaliana Using a Low-Pressure Bombardment System and GFP Movement Assay
Plasmodesmata are nanopores in the plant cell wall that allow direct cell-to-cell communication. They are key for plant growth, development, and...
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Wheat Biotechnology
Different methods and techniques have been used to increase production and improve quality in wheat since ancient times. Conventional plant breeding...
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The Production of Therapeutic Radionuclides
Most medicinal radionuclides are not naturally occurring and must therefore be produced via nuclear reactions. An understanding of both nuclear...
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Highly Efficient Genetic Transformation Methods for the Marine Oleaginous Diatom Fistulifera solaris
The oleaginous diatom Fistulifera solaris is a promising producer of biofuel owing to the high content of the lipids. A genetic transformation...
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CRISPR/Cas9 mediated editing of phytoene desaturase gene in squash
Gene editing using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated 9 (CRISPR/Cas9) system has become an important...
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Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP
Plasmodesmata (PD) play an important role in plant growth and development and defense. The permeability of PD is strictly regulated. Here, we...
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Routine and efficient in vitro regeneration system amenable to biolistic particle delivery in chickpea (Cicer arietinum L.)
Genetic engineering can add new capabilities or traits and direct method using biolistic particle delivery holds key for rapid, routine and efficient...
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Using a Hand-Held Gene Gun for Genetic Transformation of Tetrahymena thermophila
Biolistic bombardmentTetrahymenabiolistic bombardment is widely used as a means of delivering vector-coated microparticles into microorganisms,...
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Biolistic Inoculation of Fruit Trees with Full-Length Infectious cDNA Clones of RNA Viruses
Long life cycle and lack of efficient and robust virus inoculation technique are the major technical challenges for studying virus infection in...
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Advances in Delivery of CRISPR–Cas Reagents for Precise Genome Editing in Plants
The human population is growing rapidly and is projected to reach 10 billion by 2055 according to The World Bank. However, limited cultivable land,...
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PSG076 Promoter Shows Late Pollen-Specific Activity in Wheat (Triticum aestivum L.)
Pollen, as the male gametophyte of plants, plays a critical role in plant sexual reproduction. Investigating pollen-specific promoters not only helps...
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The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in Carthamus tinctorius L
BackgroundSafflower ( Carthamus tinctorius L.) is an important economic crop and a traditional medicinal material rich in flavonoids, which can...
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Transient Transformation Using Particle Bombardment for Gene Expression Analysis
Transient transformation or transient expression results in rapid and fleeting gene expression. This approach is often used as a first-tier screening...
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Genome Editing by Ribonucleoprotein Based Delivery of the Cas9 System in Plants
Clustered regularly interspaced palindromic repeat (CRISPR)-based gene editing technology has opened the doors for targeted mutagenesis in a variety...
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Biolistic Transformation of Chlamydomonas reinhardtii and Saccharomyces cerevisiae Mitochondria
Chlamydomonas reinhardtii and Saccharomyces cerevisiae are currently the two micro-organisms in which genetic transformation of mitochondria is...
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CRISPR/Cas9-based generation of mlo mutants for allelic complementation experiments to elucidate MLO function in barley
Barley ( Hordeum vulgare ) mildew locus o ( mlo ) mutants exhibit strong resistance to penetration by the powdery mildew fungus Blumeria graminis f. sp. hordei...
