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Structural Modeling of T9SS Outer Membrane Proteins and Their Complexes
The type 9 secretion system (T9SS) is a recently discovered machinery that both transports cargo proteins across the Gram-negative bacterial outer...
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Pore-Forming VDAC Proteins of the Outer Mitochondrial Membrane: Regulation and Pathophysiological Role
AbstractVoltage-dependent anion channels (VDAC1-3) of the outer mitochondrial membrane are a family of pore-forming β-barrel proteins that carry out...
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Preparation of Protein-Enriched Outer Membrane Vesicles from Escherichia Coli for In Situ Structural Biology of Outer Membrane Proteins
Bacterial outer membrane vesicles (OMVs) can be selectively enriched with one or more outer membrane proteins to allow the biophysical...
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Fluorescent Labeling of Outer Membrane Proteins Using the SpyCatcher-SpyTag System
The SpyCatcher-SpyTag system has become a popular and versatile tool for protein ligation. It is based on a small globular protein (SpyCatcher) that...
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Characterization of β-Barrel Outer Membrane Proteins and Their Interactions with Chaperones by Chemical-Crosslinking Mass Spectrometry
Chemical crosslinking-mass spectrometry (XL-MS) is an established tool that can be used to study the architecture and dynamics of proteins and...
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Purification of Borrelia burgdorferi Outer Membrane Vesicles
Bacterial outer membrane vesicles (OMVs) are spherical membrane constructs shed by gram-negative bacteria. OMVs produced by the Lyme disease pathogen...
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Examining Protein Translocation by β-Barrel Membrane Proteins Using Reconstituted Proteoliposomes
β-barrel membrane proteins populate the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts, playing significant roles in...
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Maintenance of bacterial outer membrane lipid asymmetry: insight into MlaA
The outer membrane (OM) of Gram-negative bacteria acts as an effective barrier to protect against toxic compounds. By nature, the OM is asymmetric...
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Novel functions of the ER-located Hsp40s DNAJB12 and DNAJB14 on proteins at the outer mitochondrial membrane under stress mediated by CCCP
The endoplasmic reticulum (ER) membrane provides infrastructure for intracellular signaling, protein degradation, and communication among the ER...
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Affinity Purification of Membrane β-Barrel Proteins via Biotin-Tagged Peptidiscs
β-barrel membrane proteins play a crucial role in bacterial pathogenesis and antibiotic resistance, making them a prime focus for the development of...
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Tackling the outer membrane: facilitating compound entry into Gram-negative bacterial pathogens
Emerging resistance to all available antibiotics highlights the need to develop new antibiotics with novel mechanisms of action. Most of the...
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Expression, Purification, and Cryo-EM Structural Analysis of an Outer Membrane Secretin Channel
Secretin proteins form pores in the outer membranes of Gram-negative bacteria, and as such provide a means of transporting a wide variety of...
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Formation of Amyloid-Like Conformational States of β-Structured Membrane Proteins on the Example of OMPF Porin from the Yersinia pseudotuberculosis Outer Membrane
AbstractThe work presents results of the in vitro and in silico study of formation of amyloid-like structures under harsh denaturing conditions by...
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Identification and characterisation of a major outer membrane protein from Methylacidiphilum fumariolicum SolV
The outer membrane (OM) protects Gram-negative bacteria against a hostile environment. The proteins embedded in the OM fulfil a number of tasks that...
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Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens
AbstractBacterial outer membrane vesicles (OMVs) are nanosized spheroidal particles shed by gram-negative bacteria that contain biomolecules derived...
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Characterization and immunological effect of outer membrane vesicles from Pasteurella multocida on macrophages
AbstractPasteurella multocida is an important bacterial pathogen that can cause diseases in both animals and humans. Its elevated morbidity and...
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Extraction and Purification of Outer Membrane Vesicles and Their Associated RNAs
Outer membrane vesicles (OMVs), produced by Gram negative-bacteria and sRNAs, are key players in cell-to-cell communication and interactions of...
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Generation of unfolded outer membrane protein ensembles defined by hydrodynamic properties
Outer membrane proteins (OMPs) must exist as an unfolded ensemble while interacting with a chaperone network in the periplasm of Gram-negative...
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Utilizing outer membrane proteins (OMPs) as a vaccine to manage motile Aeromonas septicemia (MAS) in Nile tilapia (Oreochromis niloticus) with evaluating the vaccine immunoprotective effectiveness
Motile aeromonads are an opportunistic and heterogenic bacteria that cause motile Aeromonas septicemia (MAS) which is accompanied with variety of...
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New Perspectives on Crosstalks Between Bacterial Regulatory RNAs from Outer Membrane Vesicles and Eukaryotic Cells
Regulatory small RNAs (sRNAs) help the bacteria to survive harsh environmental conditions by posttranscriptional regulation of genes involved in...