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Comparison of real-time PCR and nested PCR based on the HlyA gene for the detection of Listeria monocytogenes. Application on cheese samples
The aim of the present study was to compare the performance of a nested polymerase chain reaction (nPCR) and a real-time PCR based on the...
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Molecular detection and phylogenetic analysis of Borrelia Spp. In blood samples of cats and dogs by the nested-PCR method in West Azerbaijan Province, Iran
The objective of this study was to investigate the presence and genetic attributes of Borrelia spp. in cats and dogs from the West Azerbaijan...
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Sensitive poliovirus detection using nested PCR and nanopore sequencing: a prospective validation study
Timely detection of outbreaks is needed for poliovirus eradication, but gold standard detection in the Democratic Republic of the Congo takes 30 days...
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A one step nested PCR method for detection of Peronospora sparsa, the downy mildew pathogen, in boysenberry (Rubus ursinus)
Downy mildew is a major disease of boysenberries in New Zealand, caused by Peronospora sparsa. Most boysenberry plant material, including tissue...
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Bioassay-based detection of Toxoplasma gondii in free-range chickens from Khorramabad, Iran: comparison of direct microscopy and semi-nested PCR
This study aimed to investigate the occurrence of anti- Toxoplasma gondii antibodies in free-range chickens from Khorramabad, western Iran, and also...
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Establishing a Zebrafish Model for Borrelia burgdorferi Infection Using Immersion and Microinjection Methods
Borrelia burgdorferi is the spirochetal bacterium that causes Lyme disease. Even though antimicrobial sensitivity of B. burgdorferi has been widely...
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Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or 96 Tick-borne Pathogens in 48 or 96 Samples
Tick-borne pathogens (TBPs) are often detected through classical molecular tools (PCR, nested PCR, real-time PCR), but these are limited in terms of...
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Primer Design
An overview of the application of oligonucleotide design is given in relation to applications involving exploratory investigations and diagnostics....
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Simultaneous Detection and Direct Identification of Phytoplasmas in the Aster Yellows (16SrI), Clover Proliferation (16SrVI) and Stolbur (16SrXII) Groups Using a Multiplex Nested PCR Assay in Potato Plants
Phytoplasmas in the aster yellows (16SrI), clover proliferation (16SrVI) and stolbur (16SrXII) groups have a wide host range with common hosts. In...
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Identification and differentiation of soft rot and blackleg bacteria from potato using nested and multiplex PCR
The bacterial genera Pectobacterium , Dickeya and Enterobacter are plant pathogens that cause soft rot and blackleg of potato. In the present study,...
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Molecular and Serological Survey of Asymptomatic Malaria Infection in Endemic Area Sistan and Baluchestan Province in East of Iran
The presence of asymptomatic malaria infection in endemic areas and misdiagnosis can lead to failure in malaria eradication strategies. The present...
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Specific colorimetric LAMP assay for the detection of maize bushy stunt phytoplasma in corn through comparative genomics
Brazil stands out as the third largest corn producer in the world, showing self-sufficiency to supply its entire national demand. However, maize red...
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Detection and phylogenetic analysis of epidemic carp edema virus in Bei**g, China (2021–2022)
Carp edema virus (CEV) is the causative agent of carp edema virus disease (CEVD), also known as koi sleepy disease (KSD), which is a highly...
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Molecular Techniques for Testing Genetic Purity and Seed Health
With the globalization of seed trade and transgenic variety development, the application of molecular technologies for seed quality gained more...
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Nested PCR assay for specific and sensitive detection of Alternaria carthami
Alternaria leaf spot (ALS) caused by Alternaria carthami Chowdhary is one of the major threats to the cultivation of safflower in the world. The...
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Diagnosis and Detection of Soil-Borne Fungal Phytopathogens in Major Crops
Phytopathogenic soil-borne fungal species can inflict huge economic disturbances in the global agricultural sector. Soil-borne diseases, incited by...
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Rapid Amplification of cDNA Ends (RACE)
Rapid amplification of cDNA ends (RACE) or “one-sided” polymerase chain reaction (PCR) or “anchored” PCR is a polymerase chain reaction-based...
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First report of ‘Candidatus Phytoplasma asteris’ (16SrI) associated with yellows disease of Trachyspermum ammi from India
Trachyspermum ammi is a traditional medicinal plant and is widely used for curing various diseases in humans. During a 2022 survey, yellowing...
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Identification of cetaceans from bones using molecular techniques provides insights into cetacean species diversity and composition in coastal western Taiwan Strait waters, China
Molecular techniques provide powerful tools that enable accurate identification of most animal species. We amplify mitochondrial DNA fragments...
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An enzymatic recombinase amplification assay combined with CRISPR-Cas12a for the rapid detection of acute hepatopancreatic necrosis disease in shrimp Penaeus vannamei
The shrimp farming sector has faced significant hurdles due to acute hepatopancreatic necrosis disease (AHPND). In this study, an attempt was made to...
