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Integrated vector genomes may contribute to long-term expression in primate liver after AAV administration
The development of liver-based adeno-associated virus (AAV) gene therapies is facing concerns about limited efficiency and durability of transgene...
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Optimization of mammalian expression vector by cis-regulatory element combinations
The regulation of gene expression in mammalian cells by combining various cis-regulatory features has rarely been discussed. In this study, we...
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New temperature-switchable acyl homoserine lactone-regulated expression vector
AbstractBacterial expression systems play an indispensable role in the biosynthesis of recombinant proteins. Different proteins and the tasks...
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Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
BackgroundPlant virus vectors designed for virus-mediated protein overexpression (VOX), virus-induced gene silencing (VIGS), and genome editing...
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Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS)
The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our...
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Molecular cloning and expression of codon-optimized segment 4 hypothetical protein (35 kDa) of tilapia lake virus (TiLV) in pET-28a( +) expression vector and development of indirect ELISA test
Tilapia lake virus (TiLV) is a novel single-stranded RNA virus that is considered a threat to the universal tilapia industry. Recombinant technology...
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Genome Editing of Murine Liver Hepatocytes by AAV Vector-Mediated Expression of Cas9 In Vivo
Adeno-associated virus (AAV) vectors are attractive tools for gene transfer to the liver and are used as gene therapeutic drugs for inherited...
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A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean
BackgroundSoybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic...
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A minimal bile salt excretory pump promoter allows bile acid-driven physiological regulation of transgene expression from a gene therapy vector
BackgroundBile acid (BA) homeostasis is mainly regulated by bile salt excretory pump (BSEP), a hepatocyte transporter that transfers BAs to the bile....
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CRISPR/Cas9 Vector Construction for Gene Knockout
This protocol outlines the construction of a plant transformation plasmid to express both the Cas9 nuclease and individual guide RNA (gRNA),...
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An Efficient Vector-Based CRISPR/Cas9 System in Zebrafish Cell Line
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been widely applied in animals as...
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Virus–Vector Interactions and Transmission
Plant viruses cause significant economic losses in agriculture by affecting crop yield and quality. The transmission of these viruses often relies on...
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Vector-virus interaction affects viral loads and co-occurrence
BackgroundVector-borne viral diseases threaten human and wildlife worldwide. Vectors are often viewed as a passive syringe injecting the virus....
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Revolutionizing Veterinary Health with Viral Vector-Based Vaccines
Vaccines signify one of the economical and reasonable means to prevent and eradicate the important infectious diseases. Conventional vaccines like...
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Engineering Synthetic circRNAs for Efficient CNS Expression
Circular RNAs (circRNAs) have recently emerged as a promising modality for gene and RNA-based therapies. They are more stable than their linear...
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Mosquito gene targeted RNAi studies for vector control
Vector-borne diseases are serious public health concern. Mosquito is one of the major vectors responsible for the transmission of a number of...
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Prevention of severe lung immunopathology associated with influenza infection through adeno-associated virus vector administration
BackgroundInfluenza A viruses (IAVs) have long posed a threat to humans, occasionally causing significant morbidity and mortality. The initial immune...
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Spatio-temporal characterization of phenotypic resistance in malaria vector species
BackgroundMalaria, a deadly disease caused by Plasmodium protozoa parasite and transmitted through bites of infected female Anopheles mosquitoes,...
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Cloning and Recombinant Protein Expression in Lactococcus lactis
The Lactococcus lactis, a Gram-positive bacteria, is an ideal expression host for the overproduction of heterologous proteins in a properly folded...
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Molecular and immunological studies on Theileria equi and its vector in Egypt
Equine piroplasmosis is not fully understood regarding pathogenicity, prophylaxis, host immune response expression, and specific vectors. Accurately...
