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Transmembrane β-Barrel Proteins Methods and Protocols
This detailed volume explores experimental strategies and protocols for the expression, assembly, characterization, and exploitation of transmembrane...
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Analysis and prediction of interactions between transmembrane and non-transmembrane proteins
BackgroundMost of the important biological mechanisms and functions of transmembrane proteins (TMPs) are realized through their interactions with...
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Recombinant Expression and Overproduction of Transmembrane β-Barrel Proteins
Transmembrane β-barrel proteins reside in the outer membrane of Gram-negative bacteria and are thus in direct contact with the environment. Because...
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Analysis of Transmembrane β-Barrel Proteins by Native and Semi-native Polyacrylamide Gel Electrophoresis
Membrane-embedded β-barrel proteins are important regulators of the outer membrane permeability barrier of Gram-negative bacteria. β-barrels are...
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TMbed: transmembrane proteins predicted through language model embeddings
BackgroundDespite the immense importance of transmembrane proteins (TMP) for molecular biology and medicine, experimental 3D structures for TMPs...
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Fat storage-inducing transmembrane proteins: beyond mediating lipid droplet formation
Fat storage-inducing transmembrane proteins (FITMs) were initially identified in 2007 as members of a conserved endoplasmic reticulum (ER) resident...
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Rationale in Custom Design of Transmembrane β-Barrel Pores
Biological nanopores incorporated into synthetic membranes are widely used for single-molecule analytical applications such as DNA sequencing. The...
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Structure and Function of the Glycosylphosphatidylinositol Transamidase, a Transmembrane Complex Catalyzing GPI Anchoring of Proteins
Glycosylphosphatidylinositol (GPI) anchoring of proteins is a ubiquitous posttranslational modification in eukaryotic cells. GPI-anchored proteins...
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Characterization and phylogenetic analysis of multiple C2 domain and transmembrane region proteins in maize
BackgroundMultiple C2 domain and transmembrane region proteins (MCTPs) are evolutionarily conserved and important signaling molecules. However, the...
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Biological Membranes and Membrane Proteins
We focus specifically on biological membranes and membrane proteins here because the physical interactions involved in maintaining their structure...
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Structure and mechanism of lysosome transmembrane acetylation by HGSNAT
Lysosomal transmembrane acetylation of heparan sulfates (HS) is catalyzed by HS acetyl-CoA:α-glucosaminide N -acetyltransferase (HGSNAT), whose...
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The roles of transmembrane family proteins in the regulation of store-operated Ca2+ entry
Store-operated Ca 2+ entry (SOCE) is a major pathway for calcium signaling, which regulates almost every biological process, involving cell...
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Inter-helical Residue Contact Prediction in \(\alpha \) -Helical Transmembrane Proteins Using Structural Features
Residue contact maps offer a 2-d, reduced representation of 3-d protein structures and constitute a structural constraint and scaffold in structural...
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Dimeric Transmembrane Structure of the SARS-CoV-2 E Protein
The SARS-CoV-2 E protein is a transmembrane (TM) protein with its N-terminus exposed on the external surface of the virus. At debate is its...
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Expression, purification, and characterization of transmembrane protein homogentisate solanesyltransferase
AbstractHomogentisate solanesyltransferase (HST) is a crucial enzyme in the plastoquinone biosynthetic pathway and has recently emerged as a...
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Cryo-EM analysis reveals human SID-1 transmembrane family member 1 dynamics underlying lipid hydrolytic activity
Two mammalian homologs of systemic RNA interference defective protein 1 (SID-1) (SIDT1/2) are suggested to function as double-stranded RNA (dsRNA)...
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Structure, Function and Mechanism of Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) Enzymes
NADPH Oxidases (NOXs) generate reactive oxygen species (ROS) by transferring electrons from intracellular NADPH to molecular oxygen at the opposite...
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Bacterial One- and Two-Hybrid Assays to Monitor Transmembrane Helix Interactions
In transenvelope multiprotein machines such as bacterial secretion systems, protein–protein interactions not only occur between soluble domains but...
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Is cholesterol both the lock and key to abnormal transmembrane signals in Autism Spectrum Disorder?
Disturbances in cholesterol homeostasis have been associated with ASD. Lipid rafts are central in many transmembrane signaling pathways (including...
