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Discovery of NSD2 non-histone substrates and design of a super-substrate
The human protein lysine methyltransferase NSD2 catalyzes dimethylation at H3K36. It has very important roles in development and disease but many...
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Probing Substrate-Loaded Carrier Proteins by Nuclear Magnetic Resonance
Carrier proteins (CPs) are central actors in nonribosomal peptide synthetases (NRPSs) as they interact with all catalytic domains, and because they...
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Map** the determinants of catalysis and substrate specificity of the antibiotic resistance enzyme CTX-M β-lactamase
CTX-M β-lactamases are prevalent antibiotic resistance enzymes and are notable for their ability to rapidly hydrolyze the extended-spectrum...
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Organic matter decomposition in mountain peatlands: effects of substrate quality and peatland degradation
Background and AimsPeatlands occupy only 3% of Earth’s terrestrial lands but store about one-third of global soil carbon. However, these large carbon...
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Dynamic molecular architecture and substrate recruitment of cullin3–RING E3 ligase CRL3KBTBD2
Phosphatidylinositol 3-kinase α, a heterodimer of catalytic p110α and one of five regulatory subunits, mediates insulin- and insulin like growth...
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Characterization of Prolyl-4-Hydroxylase Substrate Specificity Using Pichia pastoris as an Efficient Eukaryotic Expression System
The use of eukaryotic expression systems facilitates the heterologous expression of complex eukaryotic proteins in their post-translationally...
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Transesterification with CE15 glucuronoyl esterase from Cerrena unicolor reveals substrate preferences
PurposeGlucuronoyl esterases (GE, family CE15) catalyse the cleavage of ester linkages in lignin-carbohydrate complexes (LCCs), and this study...
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Rational engineering of an elevator-type metal transporter ZIP8 reveals a conditional selectivity filter critically involved in determining substrate specificity
Engineering of transporters to alter substrate specificity as desired holds great potential for applications, including metabolic engineering....
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CAND1 inhibits Cullin-2-RING ubiquitin ligases for enhanced substrate specificity
Through targeting essential cellular regulators for ubiquitination and serving as a major platform for discovering proteolysis-targeting chimera...
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Broad Spectrum Amino Acid Racemases (Bsrs): A Potential Target in Microbial Research
Broad spectrum amino acid racemases (Bsrs) aid bacteria in producing non-canonical D-amino acids (NCDAA), which play significant functions in the...
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Mechanisms of substrate processing during ER-associated protein degradation
Maintaining proteome integrity is essential for long-term viability of all organisms and is overseen by intrinsic quality control mechanisms. The...
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Cooperation of N- and C-terminal substrate transmembrane domain segments in intramembrane proteolysis by γ-secretase
Intramembrane proteases play a pivotal role in biology and medicine, but how these proteases decode cleavability of a substrate transmembrane (TM)...
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Synthesis and application of a phenazine class substrate for high-throughput screening of laccase activity
Biocatalysis is one of the greatest tools for implementing the 12 principles of Green chemistry. Biocatalysts are bio-based, highly efficient and...
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SALL4 is a CRL3REN/KCTD11 substrate that drives Sonic Hedgehog-dependent medulloblastoma
The Sonic Hedgehog (SHH) pathway is crucial regulator of embryonic development and stemness. Its alteration leads to medulloblastoma (MB), the most...
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Methanol as a co-substrate with CO2 enhances butyrate production in microbial electrosynthesis
AbstractMethanol is a promising feedstock for the bio-based economy as it can be derived from organic waste streams or produced electrochemically...
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Use of Ozone as a Substrate Treatment for the Control of Trichoderma in the Production of Pleurotus ostreatus
Pleurotus ostreatus is one of the most widely cultivated species in the world. It can be produced in many lignocellulosic substrates after carrying...
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Substrate-borne vibrations produced during the interaction with natural enemies alter aphids probing behavior
The “ecology of fear”, i.e., physiological and behavioral alterations displayed by pests in response to predation risk, has recently been proposed as...
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Spectrum of Biological Activity of Extracts from Cultures of Distinct Isolates of Bipolaris sorokiniana Produced on Different Media
Abstract —Bipolaris sorokiniana is circumglobally widespread root and leaf pathogen of cereals and grasses. It can grow both endo- and epiphytically...
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Cryo-EM structure of GABA transporter 1 reveals substrate recognition and transport mechanism
The inhibitory neurotransmitter γ-aminobutyric acid (GABA) is cleared from the synaptic cleft by the sodium- and chloride-coupled GABA transporter...
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Targeting papain-like protease for broad-spectrum coronavirus inhibition
The emergence of SARS-CoV-2 variants of concern and repeated outbreaks of coronavirus epidemics in the past two decades emphasize the need for...
