Search
Search Results
-
Temporal analysis of relative distances (TARDIS) is a robust, parameter-free alternative to single-particle tracking
In single-particle tracking, individual particles are localized and tracked over time to probe their diffusion and molecular interactions. Temporal...
-
OPUS-DSD: deep structural disentanglement for cryo-EM single-particle analysis
Cryo-electron microscopy (cryo-EM) captures snapshots of dynamic macromolecules, collectively illustrating the involved structural landscapes. This...
-
Experimental and numerical analysis of the pyrolysis dynamics of a single wood particle: presentation of the radiographic technique
Pyrolysis is an oxygen-free process for the thermal decomposition of raw materials. The heat conduction and flow of pyrolysis products (i.e., the gas...
-
Variable-Angle Epifluorescence Microscopy for Single-Particle Tracking in the Plant ER
Single-particle tracking (SPT) of biomolecules in the plant endoplasmic reticulum has the potential to inform on the formation of protein–protein...
-
Metallo-supramolecular branched polymer protects particles from air-water interface in single-particle cryo-electron microscopy
Recent technological breakthroughs in single-particle cryo-electron microscopy (cryo-EM) enable rapid atomic structure determination of biological...
-
nextPYP: a comprehensive and scalable platform for characterizing protein variability in situ using single-particle cryo-electron tomography
Single-particle cryo-electron tomography is an emerging technique capable of determining the structure of proteins imaged within the native context...
-
High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler
We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of...
-
Uncovering structural ensembles from single-particle cryo-EM data using cryoDRGN
Single-particle cryogenic electron microscopy (cryo-EM) has emerged as a powerful technique to visualize the structural landscape sampled by a...
-
Single-particle cryo-EM structures from iDPC–STEM at near-atomic resolution
In electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM)...
-
Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models
Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we...
-
A Protocol for Studying Transcription Factor Dynamics Using Fast Single-Particle Tracking and Spot-On Model-Based Analysis
Single-particle tracking (SPT) makes it possible to directly observe single protein diffusion dynamics in living cells over time. Thus, SPT has...
-
Insights into HIV uncoating from single-particle imaging techniques
Human immunodeficiency virus (HIV) is the most extensively researched human pathogen. Despite this massive scientific endeavour, several fundamental...
-
A Single-Step Digestion for the Quantification and Characterization of Trace Particulate Silica Content in Biological Matrices Using Single Particle Inductively Coupled Plasma-Mass Spectrometry
The increased use of amorphous silica nanoparticles (SiNPs) in food products, materials science, cosmetics, and pharmaceuticals has raised questions...
-
Enhanced hexamerization of insulin via assembly pathway rerouting revealed by single particle studies
Insulin formulations with diverse oligomerization states are the hallmark of interventions for the treatment of diabetes. Here using single-molecule...
-
CASSPER is a semantic segmentation-based particle picking algorithm for single-particle cryo-electron microscopy
Particle identification and selection, which is a prerequisite for high-resolution structure determination of biological macromolecules via...
-
Single-particle virology
The development of advanced experimental methodologies, such as optical tweezers, scanning-probe and super-resolved optical microscopies, has led to...
-
Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues
Super-resolution microscopy techniques have pushed the limit of optical imaging to unprecedented spatial resolutions. However, one of the frontiers...
-
Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM
Nanobodies are popular and versatile tools for structural biology. They have a compact single immunoglobulin domain organization, bind target...
-
Influence of Linker DNA on Nucleosome Structure according to Single-Particle Fluorescence Microscopy Data
AbstractMononucleosomes assembled using DNA templates of various lengths that contain a nucleosome-positioning sequence are widely used in molecular...
-
Molecular goniometers for single-particle cryo-electron microscopy of DNA-binding proteins
Correct reconstruction of macromolecular structure by cryo-electron microscopy (cryo-EM) relies on accurate determination of the orientation of...
