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Direct visualization of transcription-replication conflicts reveals post-replicative DNA:RNA hybrids
Transcription-replication collisions (TRCs) are crucial determinants of genome instability. R-loops were linked to head-on TRCs and proposed to...
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Circular DNA intermediates in the generation of large human segmental duplications
BackgroundDuplications of large genomic segments provide genetic diversity in genome evolution. Despite their importance, how these duplications are...
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Isolation of dsRNA from Plants by Cellulose Chromatography
As the constitutive molecules of double-stranded RNA (dsRNA) virus genomes and replicative intermediates of single-stranded RNA (ssRNA) viruses, the...
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Replication fork uncoupling causes nascent strand degradation and fork reversal
Genotoxins cause nascent strand degradation (NSD) and fork reversal during DNA replication. NSD and fork reversal are crucial for genome stability...
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Identification and Characterization of Some Genes, Enzymes, and Metabolic Intermediates Belonging to the Bile Acid Aerobic Catabolic Pathway from Pseudomonas
The study of the catabolic potential of microbial species isolated from different habitats has allowed the identification and characterization of...
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Genetic perturbation of mitochondrial function reveals functional role for specific mitonuclear genes, metabolites, and pathways that regulate lifespan
Altered mitochondrial function is tightly linked to lifespan regulation, but underlying mechanisms remain unclear. Here, we report the chronological...
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Lifespan regulation by targeting heme signaling in yeast
Heme is an essential prosthetic group that serves as a co-factor and a signaling molecule. Heme levels decline with age, and its deficiency is...
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The HMCES DNA-protein cross-link functions as an intermediate in DNA interstrand cross-link repair
The 5-hydroxymethylcytosine binding, embryonic stem-cell-specific (HMCES) protein forms a covalent DNA-protein cross-link (DPC) with abasic (AP)...
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Culture-Independent Discovery of Viroids by Deep Sequencing and Computational Algorithms
Viroids are single-stranded circular RNA molecules that cause diseases in plants and do not encode any protein. Classical approaches for the...
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The enzymatic properties of Arabidopsis thaliana DNA polymerase λ suggest a role in base excision repair
Base excision repair (BER) generates gapped DNA intermediates containing a 5′-terminal 2-deoxyribose-5-phosphate (5′-dRP) group. In mammalian cells,...
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Characterization of the impact of GMP/GDP synthesis inhibition on replicative lifespan extension in yeast
Slowing down aging-associated accumulation of molecular damage or its prevention represents a promising therapeutic paradigm to combat aging-related...
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Unwinding of a eukaryotic origin of replication visualized by cryo-EM
To prevent detrimental chromosome re-replication, DNA loading of a double hexamer of the minichromosome maintenance (MCM) replicative helicase is...
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The palisade layer of the poxvirus core is composed of flexible A10 trimers
Due to its asymmetric shape, size and compactness, the structure of the infectious mature virus (MV) of vaccinia virus (VACV), the best-studied...
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Mitochondria, metabolism, and aging in yeast
Quantitative and qualitative changes in metabolism take place when the lifespan is extended in yeast either by genetic or nutritional manipulation....
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Replication stress as a driver of cellular senescence and aging
Replication stress refers to slowing or stalling of replication fork progression during DNA synthesis that disrupts faithful copying of the genome....
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Genetic approaches to improve clorobiocin production in Streptomyces roseochromogenes NRRL 3504
Streptomyces roseochromogenes NRRL 3504 is best known as a producer of clorobiocin, a DNA replication inhibitor from the aminocoumarin family of...
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Evolutionary diversification of methanotrophic ANME-1 archaea and their expansive virome
‘ Candidatus Methanophagales’ (ANME-1) is an order-level clade of archaea responsible for anaerobic methane oxidation in deep-sea sediments. The...
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Epigenetic Reprogramming of the Glucose Metabolic Pathways by the Chromatin Effectors During Cancer
Glucose metabolism plays a vital role in regulating cellular homeostasis as it acts as the central axis for energy metabolism, alteration in which...
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Give and Take: The Reciprocal Control of Metabolism and Cell Cycle
Cell cycle is an ordered sequence of events that occur in a cell preparing for cell...
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Sources, resolution and physiological relevance of R-loops and RNA–DNA hybrids
RNA–DNA hybrids are generated during transcription, DNA replication and DNA repair and are crucial intermediates in these processes. When RNA–DNA...
