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Quantification of arbuscular mycorrhizal fungi root colonization in wheat, tomato, and leek using absolute qPCR
Arbuscular mycorrhizal fungi (AMF) form symbioses with most terrestrial plants and are known to have a positive effect on plant growth and health....
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Quantifying bias introduced by sample collection in relative and absolute microbiome measurements
To gain insight into the accuracy of microbial measurements, it is important to evaluate sources of bias related to sample condition, preservative...
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Integrating Identification and Quantification Uncertainty for Differential Protein Abundance Analysis with Triqler
Protein quantification for shotgun proteomics is a complicated process where errors can be introduced in each of the steps. Triqler is a Python...
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Multivariate statistical regression analysis and relative quantification based on dimensional-reduction method to compare the taste-active components of different chicken breeds
To compare the difference of non-volatile taste-active flavor components of different chicken breeds, four Chinese native yellow feather broilers...
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Simultaneous detection and quantification by multiplex qPCR of 'Candidatus Phytoplasma asteris' and 'Candidatus Phytoplasma fraxini' in a plant host and insect vectors
Phytoplasmas are bacteria transmitted by insects that can cause plant diseases. In Bogotá ' Candidatus Phytoplasma asteris' and ' Candidatus ...
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Relative Quantification of Residue-Specific m6A RNA Methylation Using m6A-RT-QPCR
Technological advances in high-throughput sequencing in combination with antibody enrichment and/or induced nucleotide-specific chemical...
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Context-aware transcript quantification from long-read RNA-seq data with Bambu
Most approaches to transcript quantification rely on fixed reference annotations; however, the transcriptome is dynamic and depending on the context,...
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Fast, Free, and Flexible Peptide and Protein Quantification with FlashLFQ
The rapid and accurate quantification of peptides is a critical element of modern proteomics that has become increasingly challenging as proteomic...
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Systematic assessment of long-read RNA-seq methods for transcript identification and quantification
The Long-read RNA-Seq Genome Annotation Assessment Project Consortium was formed to evaluate the effectiveness of long-read approaches for...
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Genoty** by sequencing for estimating relative abundances of diatom taxa in mock communities
BackgroundDiatoms are present in all waters and are highly sensitive to pollution gradients. Therefore, they are ideal bioindicators for water...
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Camalexin quantification using HPLC and the novel internal standard thiabendazole
Camalexin is the key phytoalexin in Arabidopsis thaliana and crops such as Camelina sativa . It is typically quantified by liquid chromatographic...
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A methylene blue assay for the quantification of fucoidan – A specific application for seaweed extracts
Fucoidan is a fucose-rich sulfated polysaccharide found mainly in brown seaweeds. Fucoidan has recently gained much attention due to its bioactive...
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Droplet Digital PCR for the Detection and Quantification of Bona Fide CircRNAs
CircRNAs are covalently closed RNA molecules gaining increasing attention over the years. Initially considered mere splicing errors, circRNAs are now...
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Assembly-Free Detection and Quantification of Transposable Elements with dnaPipeTE
The detection and quantification of transposable elements (TE) are notoriously challenging despite their relevance in evolutionary genomics and...
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Eggsplorer: a rapid plant–insect resistance determination tool using an automated whitefly egg quantification algorithm
BackgroundA well-known method for evaluating plant resistance to insects is by measuring insect reproduction or oviposition. Whiteflies are vectors...
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NMR metabolite quantification of a synthetic urine sample: an inter-laboratory comparison of processing workflows
IntroductionAbsolute quantification of individual metabolites in complex biological samples is crucial in targeted metabolomic profiling.
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Selective Quantification of Chemotropic Responses of Fusarium graminearum
Chemotropism refers to the directional growth of a living organism toward a chemical stimulus. Molecular mechanisms underlying chemotropism of fungal...
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Innovative Quantification of Critical Quality Attributes
Potency testing is an important part of the evaluation of cellular therapy products. In vitro quantification of identified quality-related biomarkers...
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Development of a spectrophotometric method for quantification of C-phycocyanin in the cyanobacterium Aphanizomenon flos-aquae
This study presents the development of a reliable method for quantifying C-phycocyanin. It was found that the spectrophotometric method commonly used...
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Extended automated quantification algorithm (AQuA) for targeted 1H NMR metabolomics of highly complex samples: application to plant root exudates
IntroductionThe Automated Quantification Algorithm (AQuA) is a rapid and efficient method for targeted NMR-based metabolomics, currently optimised...
