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Construction of Pseudomonas aeruginosa SDK-6 with synthetic lipase gene cassette and optimization of different parameters using response surface methodology for over-expression of recombinant lipase
Lipases are industrially important enzymes having vast applications in various fields. Cloning and expression of lipase enzyme-encoding genes in...
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Recombinant Strains Producing Thermomyces lanuginosus Thermostable Lipase and their Use in Heterogeneous Biocatalysis, Including Processes of Low-Temperature Synthesis of Esters
This work was devoted to the construction of recombinant strains Escherichia coli BL21 (DE3) and Pichia pastoris X33, which produce a 1,3-specific...
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Characterization of a recombinant Aspergillus niger GZUF36 lipase immobilized by ionic liquid modification strategy
AbstractEnzyme immobilized on magnetic nanomaterials is a promising biocatalyst with efficient recovery under applied magnets. In this study, a...
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Assay of Monoacylglycerol Lipase Activity
Monoacylglycerol lipase (MGL/MAGL/MGLL) is a serine hydrolase involved in the biological deactivation of the endocannabinoid...
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Modulation of the Catalytic Properties of Immobilized Recombinant Lipase from Thermomyces lanuginosus in the Reaction of Esterification by the Selection of an Adsorbent
AbstractBiocatalysts with lipase activity (BLAs) were prepared by adsorptive immobilization of recombinant lipase (r Pichia /lip) from thermophilic...
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Cloning, Overexpression and Application of Lipase from Thermotolerant Bacillus subtilis TTP-06 in the Degradation of Polyhydroxyalkanoate
Polyhydroxyalkanoates (PHAs) constitute a principal group of bio-degradable polymers that are produced by certain microbes under limited supply of...
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Macromolecular Interactions of Lipoprotein Lipase (LPL)
Lipoprotein lipase (LPL) is a critical enzyme in humans that provides fuel to peripheral tissues. LPL hydrolyzes triglycerides from the cores of...
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Purification and biochemical characterization of extracellular thermostable lipase from Bacillus sp. strain L2
In the current study, an extracellular thermostable lipase from Bacillus sp. strain L2 was produced and purified through two-steps purifications,...
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Recent advances in the production, properties and applications of haloextremozymes protease and lipase from haloarchaea
Proteases and lipases are significant groups of enzymes for commercialization at the global level. Earlier, the industries depended on mesophilic...
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Shifting the pH profiles of Staphylococcus epidermidis lipase (SEL) and Staphylococcus hyicus lipase (SHL) through generating chimeric lipases by DNA shuffling strategy
Enzymes are often required to function in a particular reaction condition by the industrial procedure. In order to identify critical residues...
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Lipase-mediated detoxification of host-derived antimicrobial fatty acids by Staphylococcus aureus
Long-chain fatty acids with antimicrobial properties are abundant on the skin and mucosal surfaces, where they are essential to restrict the...
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Knotting terminal ends of mutant T1 lipase with disulfide bond improved structure rigidity and stability
AbstractLipase biocatalysts offer unique properties which are often impaired by low thermal and methanol stability. In this study, the rational...
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Lipase domain effector AGLIP1 in Rhizoctonia solani triggers necrotic killing in plants
Key messageWe highlight the emerging role of the R. solani novel lipase domain effector AGLIP1 in suppressing pattern-triggered immunity and...
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Screening, identification, and characterization of lipase-producing halotolerant Bacillus altitudinis Ant19 from Antarctic soil
Potent lipase-producing and halotolerant Bacillus altitudinis Ant19 strain was screened and isolated from Antarctic soil. The isolate showed...
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A lipase from Lacticaseibacillus rhamnosus IDCC 3201 with thermostability and pH resistance for use as a detergent additive
AbstractLipases are important biocatalysts and ubiquitous in plants, animals, and microorganisms. The high growth rates of microorganisms with low...
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Screening, optimization, and molecular characterization of cold-active lipase producing Bacillus cereus I13 from Arctic sediments
Cold-active enzymes are extremozymes produced by psychrophiles, and have attracted much attention as biocatalysts due to their capacity to resist...
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Enhanced enzyme thermostability of a family I.3 lipase LipSR1 by T118A mutation at the calcium-binding site
ObjectivesThe lipase gene lipSR1 isolated from oil-contaminated soil exhibits high hydrolytic activity for short-chain fatty acid substrates. A...
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Y12F mutation in Pseudomonas plecoglossicida S7 lipase enhances its thermal and pH stability for industrial applications: a combination of in silico and in vitro study
Appropriate amino acid substitutions are critical for protein engineering to redesign catalytic properties of industrially important enzymes like...
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Recombinant therapeutic proteins degradation and overcoming strategies in CHO cells
AbstractMammalian cell lines are frequently used as the preferred host cells for producing recombinant therapeutic proteins (RTPs) having...
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A novel acidic and SDS tolerant halophilic lipase from moderate halophile Nesterenkonia sp. strain F: molecular cloning, structure analysis and biochemical characterization
A novel gene encoding an acidic and SDS tolerant halophilic lipase from moderately halophilic bacterium, Nesterenkonia sp. strain F ( lip F) was cloned...
