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  1. RNA-Seq Data Analysis

    RNA-Seq data analysis stands as a vital part of genomics research, turning vast and complex datasets into meaningful biological insights. It is a...
    James Li, Rency S. Varghese, Habtom W. Ressom in RNA Amplification and Analysis
    Protocol 2024
  2. Short-Read RNA-Seq

    RNA sequencing (RNA-Seq) has emerged as a powerful and versatile tool for the comprehensive analysis of transcriptomes and has been widely used to...
    Rong Hu, Md N. Islam, ... Habtom W. Ressom in RNA Amplification and Analysis
    Protocol 2024
  3. RNA-clique: a method for computing genetic distances from RNA-seq data

    Background

    Although RNA-seq data are traditionally used for quantifying gene expression levels, the same data could be useful in an integrated...

    Andrew C. Tapia, Jerzy W. Jaromczyk, ... Christopher L. Schardl in BMC Bioinformatics
    Article Open access 04 June 2024
  4. Metatranscriptomic RNA-Seq Data Analysis of Virus-Infected Host Cells

    RNA sequencing (RNA-seq)RNA sequencing (RNA-seq) analysis of virus-infected host cells enables researchers to study a wide range of phenomena...
    Nooran Abu Mazen, Jessica Luc, ... Andrew C. Doxey in Intracellular Pathogens
    Protocol 2024
  5. A comprehensive workflow for optimizing RNA-seq data analysis

    Background

    Current RNA-seq analysis software for RNA-seq data tends to use similar parameters across different species without considering...

    Gao Jiang, Juan-Yu Zheng, ... Hou-Ling Wang in BMC Genomics
    Article Open access 24 June 2024
  6. Computational Analysis of Single-Cell RNA-Seq Data

    Single-cell RNA sequencing (scRNA-seq) is gaining popularity as this allows you to profile a large number of individual cells. However, as the volume...
    Protocol 2023
  7. Benchmarking algorithms for joint integration of unpaired and paired single-cell RNA-seq and ATAC-seq data

    Background

    Single-cell RNA-sequencing (scRNA-seq) measures gene expression in single cells, while single-nucleus ATAC-sequencing (snATAC-seq)...

    Michelle Y. Y. Lee, Klaus H. Kaestner, Mingyao Li in Genome Biology
    Article Open access 24 October 2023
  8. Targeting nucleotide metabolic pathways in colorectal cancer by integrating scRNA-seq, spatial transcriptome, and bulk RNA-seq data

    Background

    Colorectal cancer is a malignant tumor of the digestive system originating from abnormal cell proliferation in the colon or rectum, often...

    Songyun Zhao, Pengpeng Zhang, ... Peihua Lu in Functional & Integrative Genomics
    Article Open access 10 April 2024
  9. Curare and GenExVis: a versatile toolkit for analyzing and visualizing RNA-Seq data

    Even though high-throughput transcriptome sequencing is routinely performed in many laboratories, computational analysis of such data remains a...

    Patrick Blumenkamp, Max Pfister, ... Alexander Goesmann in BMC Bioinformatics
    Article Open access 29 March 2024
  10. RNA Sequencing (RNA-seq)

    RNA sequencing (or popularly known as RNA-seq) or whole transcriptome shortgun sequencing (WTSS) is a useful next-generation sequencing (NGS)...
    Protocol 2022
  11. KARR-seq reveals cellular higher-order RNA structures and RNA–RNA interactions

    RNA fate and function are affected by their structures and interactomes. However, how RNA and RNA-binding proteins (RBPs) assemble into higher-order...

    Tong Wu, Anthony Youzhi Cheng, ... Chuan He in Nature Biotechnology
    Article Open access 18 January 2024
  12. RNA-Seq Experiment and Data Analysis

    With the ability to obtain several millions of reads per sample, high-throughput RNA sequencing (RNA-Seq) enables investigation of any transcriptome...
    Miyuraj Harishchandra Hikkaduwa Withanage, Hanquan Liang, Erliang Zeng in Estrogen Receptors
    Protocol 2022
  13. Integrative analysis of Iso-Seq and RNA-seq data reveals transcriptome complexity and differential isoform in skin tissues of different hair length Yak

    Background

    The hair follicle development process is regulated by sophisticated genes and signaling networks, and the hair grows from the hair...

    Xuelan Zhou, **aoyun Wu, ... ** Yan in BMC Genomics
    Article Open access 21 May 2024
  14. RNA Preparation and RNA-Seq Bioinformatics for Comparative Transcriptomics

    The principal transcriptome analysis is the determination of differentially expressed genes across experimental conditions. For this, the...
    Antonio Rodríguez-García, Alberto Sola-Landa, Carlos Barreiro in Microbial Steroids
    Protocol 2023
  15. A sco** review on deep learning for next-generation RNA-Seq. data analysis

    In the last decade, transcriptome research adopting next-generation sequencing (NGS) technologies has gathered incredible momentum amongst functional...

    Diksha Pandey, P. Onkara Perumal in Functional & Integrative Genomics
    Article 21 April 2023
  16. Modeling group heteroscedasticity in single-cell RNA-seq pseudo-bulk data

    Group heteroscedasticity is commonly observed in pseudo-bulk single-cell RNA-seq datasets and its presence can hamper the detection of differentially...

    Yue You, Xueyi Dong, ... Charity W. Law in Genome Biology
    Article Open access 05 May 2023
  17. Effective methods for bulk RNA-seq deconvolution using scnRNA-seq transcriptomes

    Background

    RNA profiling technologies at single-cell resolutions, including single-cell and single-nuclei RNA sequencing (scRNA-seq and snRNA-seq,...

    Francisco Avila Cobos, Mohammad Javad Najaf Panah, ... Pavel Sumazin in Genome Biology
    Article Open access 01 August 2023
  18. rMATS-turbo: an efficient and flexible computational tool for alternative splicing analysis of large-scale RNA-seq data

    Pre-mRNA alternative splicing is a prevalent mechanism for diversifying eukaryotic transcriptomes and proteomes. Regulated alternative splicing plays...

    Yuanyuan Wang, Zhijie **e, ... Yi **ng in Nature Protocols
    Article 23 February 2024
  19. Cross-platform normalization enables machine learning model training on microarray and RNA-seq data simultaneously

    Large compendia of gene expression data have proven valuable for the discovery of novel biological relationships. Historically, most available RNA...

    Steven M. Foltz, Casey S. Greene, Jaclyn N. Taroni in Communications Biology
    Article Open access 25 February 2023
  20. scFSNN: a feature selection method based on neural network for single-cell RNA-seq data

    While single-cell RNA sequencing (scRNA-seq) allows researchers to analyze gene expression in individual cells, its unique characteristics like...

    Minjiao Peng, Baoqin Lin, ... Bingqing Lin in BMC Genomics
    Article Open access 08 March 2024
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