Search
Search Results
-
RNA Folding Methods and Protocols
To understand and predict the behavior and function of RNAs, sophisticated tools are required to simulate and analyze their potential for forming...
-
RNA Amplification and Analysis Methods and Protocols
This volume provides methods to analyze RNA and gain insight to a number of critical cellular functions. Chapters detail RNA isolation and...
-
Double-Stranded RNA Methods and Protocols
This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation,...
-
Defining Bacterial RNA-RNA Interactomes Using CLASH
Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterial pathogen accounting for high mortality rates among infected patients....
-
Mechanisms and functions of RNA-guided RNA modification
RNA-guided 2’-O-methylations and pseudouridylations occur in several different types of RNAs and in a wide range of organisms. Hundreds of the RNAs...
-
RNA Structure and Function
This book focuses on the current status of our understanding of RNA, a key biological molecule. The various RNAs covered are messenger RNA, ribosomal...
-
Short-Read RNA-Seq
RNA sequencing (RNA-Seq) has emerged as a powerful and versatile tool for the comprehensive analysis of transcriptomes and has been widely used to...
-
Transkription: Biosynthese der RNA
Bei der Transkription wird die Nucleotidsequenz des DNA-Matrizenstrangs (-Strangs) eines Gens durch Basenpaarung in einen in 5′→ 3′-Richtung...
-
Experimental Validation of RNA–RNA Interactions by Electrophoretic Mobility Shift Assay
Regulatory RNAs in bacteria are known to act by base pairing with other RNAs. Interactions between two partner RNAs can be investigated by...
-
Magic RNA
About half of the human genomeGenomes is actively transcribed as RNA, new regulatory and non-protein-coding RNA types such as miRNAs and lncRNAs in...
-
RNA Isolation from Ctenophores
RNA-seq or transcriptome analysis of individual cells and small cell populations is essential for virtually any biomedical field. Here, we examine...
-
RNA Helicase
HelicaseHelicase and polymerasePolymerase are coupling in the SARS-CoV-2 replication-transcription complexReplication-transcription complex (RTC)...
-
Comparative RNA Genomics
Over the last quarter of a century it has become clear that RNA is much more than just a boring intermediate in protein expression. Ancient RNAs...
-
RNA Secondary Structure Thermodynamics
Several different ways to predict RNA secondary structures have been suggested in the literature. Statistical methods, such as those that utilize...
-
KARR-seq reveals cellular higher-order RNA structures and RNA–RNA interactions
RNA fate and function are affected by their structures and interactomes. However, how RNA and RNA-binding proteins (RBPs) assemble into higher-order...
-
Small Interfering RNA
The central dogma of molecular biology states that DNA is transcribed to RNA, which in turn is translated to protein. However, only about 2% of RNAs...
-
RNA Folding, Mutation, and Detection
The structure of RNA molecules is absolutely critical to their functions in a biological system. RNA structure is dynamic and changes in response to...
-
Bacterial Regulatory RNA Methods and Protocols
This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail...
-
RNA-Dependent RNA Polymerase (RdRp)
This chapter firstly did the work skillfully and locally by optimizing the structures of RNA-dependent RNA polymerase (RdRp) in complex with its...
-
Generation of Functional-RNA Arrays by In Vitro Transcription and In Situ RNA Capture for the Detection of RNA-RNA Interactions
RNA performs a wide variety of vital cellular functions. These functions typically require interactions with other biological macromolecules, often...