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Therapeutic Applications of Self-assembled Indole-3-butanoyl-polyethylenimine Nanostructures
This study demonstrates the therapeutic potential of indole-3-butanoyl-polyethylenimine (IBP) nanostructures formed via self-assembly in aqueous...
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Polyethylenimine linked with chitosan improves astaxanthin production in Haematococcus pluvialis
AbstractAstaxanthin is receiving increasing interest as an antioxidant and high value-added secondary metabolite. Haematococcus pluvialis is the...
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25KDa branched polyethylenimine increases interferon-γ production in natural killer cells via improving translation efficiency
BackgroundEx vivo cultivation is a promising strategy for increasing the number of NK cells and enhancing their antitumor activity prior to clinical...
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Bioluminescent Toxicity Assay of Polyethylenimine-Based Sorbents
AbstractThe toxicity of polyethylenimine-based sorbents and their extracts was evaluated, and their effect on the bioluminescence of Photobacterium...
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Chitosan nanoparticles synthesis and surface modification using histidine/ polyethylenimine and evaluation of their gene transfection efficiency in breast cancer cells
Chitosan has potential to be used as biocompatible and biodegradable non-viral gene carrier. Chitosan nanoparticles (NPs) were synthesized using...
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Effect of DMPEI coating against biofilm formation on PVC catheter surface
Urinary tract infections (UTIs) are a significant cause of morbidity in healthcare systems and are prominently associated with applying urethral...
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Bacterial magnetic particles-polyethylenimine vectors deliver target genes into multiple cell types with a high efficiency and low toxicity
AbstractBacterial magnetic particles (BMPs) are biosynthesized magnetic nano-scale materials with excellent dispersibility and biomembrane enclosure...
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High-yield production in Escherichia coli and convenient purification of a candidate vaccine against SARS-CoV-2
ObjectivesThe aim of the present work was to identify a time-saving, effective, and low-cost strategy to produce in Escherichia coli a protein...
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Polymeric nanoparticles for DNA vaccine-based cancer immunotherapy: a review
Cancer is one of the leading causes of death and mortality in the world. There is an essential need to develop new drugs or therapeutic approaches to...
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A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells
Base editors, mostly cytidine base editors (CBEs) and adenine base editors (ABEs), are powerful tools for precise base editing. However, current base...
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Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor
CRISPR-cas9-guided adenine base editors (ABEs) site-specifically convert the A-T base pair to G-C base pair in genomic DNA. The intracellular...
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Design of SaCas9-HF for In Vivo Gene Therapy
Genome alteration results in several diseases for which therapeutics are limited. Gene editing provides a strong and potential alternative for the...
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Imaging Chromatin Accessibility by Assay of Transposase-Accessible Chromatin with Visualization
Chromatin accessibility is one of the fundamental structures regulating genome functions including transcription and DNA repair. Recent technological...
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Radiometric Assay of ABHD2 Activity
The α,β-hydrolase fold-containing protein 2 (ABHD2) is a serine hydrolase, responsible for the cleavage of endogenous 2-arachidonoylglycerol (2-AG)....
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Generation, Characterization, and Count of Fluorescent Extracellular Vesicles
Extracellular vesicles (EVs) are membranous particles released by all cells in the external milieu. Depending on their origin, they are given...
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Promoting genotype-independent plant transformation by manipulating developmental regulatory genes and/or using nanoparticles
Key messageThis review summarizes the molecular basis and emerging applications of developmental regulatory genes and nanoparticles in plant...
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Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG- and HA-Tagged Bait Proteins
Proteins often interact with each other to form complexes and play functional roles in almost all cellular processes. The study of protein–protein...
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CUT&Tag Using “Stress-Free” Con A-Conjugated Dynabeads®
Epigenome researchEpigenome research has employed various methods to identify the genomic location of proteins of interest, such as transcription...
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Engineering Epithelial–Mesenchymal Microtissues to Study Cell–Cell Interactions in Development
Intercellular signaling drives human development, but there is a paucity of in vitro models that recapitulate important tissue architecture while...
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A human antibody derived from original SARS-CoV-2 infection effectively neutralizes omicron
SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) Variants of Concern (VOCs), such as the Omicron sub-variants, present significant...
