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Localized calcium transients in phragmoplast regulate cytokinesis of tobacco BY-2 cells
Key messagePlants exhibit a unique pattern of cytosolic Ca 2+ dynamics to correlate with microtubules to regulate cytokinesis, which significantly...
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A Quantitative Method for Evaluating Phragmoplast Morphology
Phragmoplasts are plant-specific microtubule structures that form cell plates at the cell division plane. During late anaphase, phragmoplasts emerge... -
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Rice kinesin-related protein STD1 and microtubule-associated protein MAP65-5 cooperatively control microtubule bundling
Main conclusions STD1 specifically interacts with MAP65-5 in rice and they cooperatively control microtubule bundles in phragmoplast expansion during...
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Overexpression of TWO-IN-ONE Domains Inhibits Cytokinesis in Arabidopsis
Plant cells undergoing cytokinesis build a cell plate between daughter nuclei and expand it centrifugally toward the parental plasma membrane. This...
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Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic Tobacco BY-2 Cells
Transgenic tobacco BY-2 cell lines stably expressing fluorescent protein-tagged marker proteins have been used to visualize the dynamic behaviors of... -
IQ67 DOMAIN proteins facilitate preprophase band formation and division-plane orientation
Spatiotemporal control of cell division is essential for the growth and development of multicellular organisms. In plant cells, proper cell plate...
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Fine map** of the reduced height gene Rht22 in tetraploid wheat landrace Jianyangailanmai (Triticum turgidum L.)
Key messageRht22 was fine mapped in the interval of 0.53–1.48 Mb on 7AS, which reduces cell number of internode to cause semi-dwarfism in...
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Dynamics of mitochondrial distribution during development and asymmetric division of rice zygotes
Key messageMitochondria change their distribution from nuclear peripheral to uniformly distributed in cytoplasm during zygotic development of rice,...
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Role of the BUB3 protein in phragmoplast microtubule reorganization during cytokinesis
The evolutionarily conserved WD40 protein budding uninhibited by benzimidazole 3 (BUB3) is known for its function in spindle assembly checkpoint...
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Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana)
The production of tissues and organs in plants is brought about by mitotic cell divisions, starting from the zygote. Successful mitosis and... -
Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens
Protonemata of the moss Physcomitrium patens are ideal structures in which to observe cytoskeletal organization and dynamics. Special care is needed... -
Exogenous 6-benzylaminopurine inhibits tip growth and cytokinesis via regulating actin dynamics in the moss Physcomitrium patens
Main conclusionExogenous BAP but not 2iP disrupts actin structures and induces tip-growth retardation and cytokinesis failure in the moss Physcomitriu...
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Methods to Visualize the Actin Cytoskeleton During Plant Cell Division
Cell division in plants consists of separating the mother cell in two daughter cells by the centrifugal growth of a new wall. This process involves... -
Algae from Primary Endosymbioses
TheRhodophyta red algaeRed algae are a group of important photoautotrophic organisms that include unicellular microalgae as well as large macroalgae... -
Importance of Cytoskeleton and Cell Wall in Somatic Embryogenesis
Both the cytoskeleton composed of microtubules and actin microfilaments as well as cell wall components such as arabinogalactan proteins and... -
Pollen Development, a Genetic and Transcriptomic View
The haploid gametophyte generation occupies a short but vital phase in the life cycle of flowering plants. The male gametophyte consists of just... -
Cell cycle-dependent dynamics of a plant intermediate filament motif protein with intracellular localization related to microtubules
Although intermediate filaments (IFs) are biochemically and immunologically suggested to exist in plant cells, there are few molecular genetic...
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Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching During Cytokinesis
Membrane trafficking is central to cell plate construction during plant cytokinesis. Studies on cell plate formation can provide answers to basic...