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Continuous Bioprocessing for Upstream Process: Perfusion Process
Perfusion processes are gaining higher interest in the industry for the production of glycoproteins. The intensification that this operation mode can...
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WAVE-based intensified perfusion cell culture for fast process development
ObjectiveThis study was to evaluate the feasibility of using a rocking type bioreactor system, specifically the WAVE 25, in an intensified perfusion...
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Attached Biofilm Cultivation (ABC) of Mixotrophic Microalgae for the Sustainable Supply of Innovative New Bioproducts
The industrial utility of microalgae is critically constrained by high-volume, monotrophic suspension approaches to biomass cultivation. Suspension...
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Optimization of an adenovirus-vectored zoster vaccine production process with chemically defined medium and a perfusion system
ObjectivesCells grown in chemically defined medium are sensitive to shear force, potentially resulting in decreased cell growth. We optimized the...
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Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system
Purpose3D cell culture and hypoxia have been demonstrated to increase the therapeutic effects of mesenchymal stem/stromal cells (MSCs)-derived...
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Investigation of osteogenesis and angiogenesis in perfusion bioreactors using improved multi-layer PCL-nHA-nZnO electrospun scaffolds
PurposeBone tissue engineering aims to create a three-dimensional, matured, angiogenic scaffold with a suitable thickness that resembles a natural...
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Development of a perfusion process for serum-free adenovirus vector herpes zoster vaccine production
Herpes zoster is caused by reactivation of the varicella zoster virus (VZV). Researching and develo** a herpes zoster vaccine will help to decrease...
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Cell culture–based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration system
Respiratory diseases including influenza A virus (IAV) infections represent a major threat to human health. While the development of a vaccine...
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Production of an anti-TNFα antibody in murine myeloma cells by perfusion culture
AbstractInfliximab is a mouse/human chimeric IgG1 monoclonal antibody which recognizes the proinflammatory cytokine, tumor necrosis factor α (TNFα),...
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High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells
AbstractSimilar to the recent COVID-19 pandemic, influenza A virus poses a constant threat to the global community. For the treatment of flu disease,...
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Production of recombinant vesicular stomatitis virus-based vectors by tangential flow depth filtration
AbstractCell culture-based production of vector-based vaccines and virotherapeutics is of increasing interest. The vectors used not only retain their...
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A comprehensive review on the heterotrophic production of bioactive compounds by microalgae
Bioactive compounds derived from microalgae have garnered considerable attention as valuable resources for drugs, functional foods, and cosmetics....
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Production of antiviral “OP7 chimera” defective interfering particles free of infectious virus
AbstractDefective interfering particles (DIPs) of influenza A virus (IAV) are suggested for use as broad-spectrum antivirals. We discovered a new...
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Large-Scale Production of Specialized Metabolites In Vitro Cultures
For centuries plants have been intensively utilized as reliable sources of food, flavoring, and pharmaceutical ingredients. However, plant natural...
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Performance of an acoustic settler versus a hollow fiber–based ATF technology for influenza virus production in perfusion
AbstractProcess intensification and integration is crucial regarding an ever increasing pressure on manufacturing costs and capacities in biologics...
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Mesenchymal and induced pluripotent stem cell–based therapeutics: a comparison
AbstractStem cell–based cell therapeutics and especially those based on human mesenchymal stem cells (hMSCs) and induced pluripotent stem cells...
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Production of retroviral vectors in continuous high cell density culture
AbstractRetroviral vectors derived from murine leukemia virus (MLV) are used in somatic gene therapy applications e.g. for genetic modification of...
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Matrix-free human pluripotent stem cell manufacturing by seed train approach and intermediate cryopreservation
BackgroundHuman pluripotent stem cells (hPSCs) have an enormous therapeutic potential, but large quantities of cells will need to be supplied by...
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Cell Culture Conditions: Cultivation of Stem Cells Under Dynamic Conditions
A major challenge in tissue engineering and cell-therapy-based approaches is the production of a significant amount of functional cells. Although it...
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Ameliorating and refining islet organoids to illuminate treatment and pathogenesis of diabetes mellitus
Diabetes mellitus, a significant global public health challenge, severely impacts human health worldwide. The organoid, an innovative in vitro...
