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β-glucosidase production by recombinant Pichia pastoris strain Y1433 under optimal feed profiles of fed-batch cultivation
Pichia pastoris , a methylotrophic yeast, is known to be an efficient host for heterologous proteins production. In this study, a recombinant P....
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Heterologous Gene Expression in Pichia pastoris: Success Stories and Commercial Ventures
Heterologous protein production in the methylotrophic yeast, Pichia pastoris, has gained immense popularity owing to the growing demands for...
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Engineering protein translocation and unfolded protein response enhanced human PH-20 secretion in Pichia pastoris
AbstractHyaluronidases catalyze the degradation of hyaluronan (HA), which is finding rising applications in medicine, cosmetic, and food industries....
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Efficient heterologous expression of bovine lactoferrin in Pichia pastoris and characterization of antibacterial activity
The low-expression level of lactoferrin (LF) in the production process poses a significant challenge. This study aimed to efficiently express bovine...
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Heterologous expression of pediocin PA-1 in Pichia pastoris: cloning, expression, characterization, and application in pork bologna preservation
ObjectivePediocin PA-1, an antimicrobial peptide derived from Pediococcus acidilactici PAC1.0, has a potential application as a food preservative...
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Visualizing Reversible Cisternal Stacking in Budding Yeast Pichia pastoris
Cisternal stacking is reversible, initiated at the “cis” side of the Golgi, and gets undone at the “trans” side in a continuous cycle in tune with...
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Aromatic secondary metabolite production from glycerol was enhanced by amino acid addition in Pichia pastoris
AbstractAromatic secondary metabolites are widely used in various industries, including the nutraceutical, dietary supplement, and pharmaceutical...
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Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system
Yeasts serve as exceptional hosts in the manufacturing of functional protein engineering and possess industrial or medical utilities. Considerable...
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Improving glutathione production by engineered Pichia pastoris: strain construction and optimal precursor feeding
Glutathione (GSH) is a metabolite that plays an important role in the fields of pharmacy, food, and cosmetics. Thus, it is necessary to increase its...
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De novo biosynthesis of 2′-fucosyllactose in engineered Pichia pastoris
PurposePichia pastoris is well known for its ability to produce short and low-immunogenic humanized glycosyl chains onto recombinant glycoproteins,...
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Production of Single-Domain Antibodies in Pichia pastoris
Single-domain antibodies (sdAbs) are binders that consist of a single immunoglobulin domain. SdAbs have gained importance as therapeutics, diagnostic...
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Membrane Protein Production in the Yeast P. pastoris
The first crystal structures of recombinant mammalian membrane proteins were solved using high-quality protein that had been produced in yeast cells....
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Recombinant expression of IGF-1 and LR3 IGF-1 fused with xylanase in Pichia pastoris
AbstractInsulin-like growth factor-1 (IGF-1) is a pleiotropic protein hormone and has become an attractive therapeutic target because of its multiple...
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Is heterogeneity in large-scale bioreactors a real problem in recombinant protein synthesis by Pichia pastoris?
AbstractCulture medium heterogeneity is inherent in industrial bioreactors. The loss of mixing efficiency in a large-scale bioreactor yields to the...
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Mini-Bioreactor Platform for Membrane Protein Production in Komagataella pastoris
Membrane proteins (MPs) play vital roles across various cellular functions, biological processes, physiological signaling pathways, and human-related...
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The Expression of Recombinant Human Hepatocyte Growth Factor in Pichia pastoris
AbstractThe aim of this study was to construct a stable and efficient eukaryotic expression system for the secretion of biologically active...
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Pathway engineering facilitates efficient protein expression in Pichia pastoris
AbstractPichia pastoris has been recognized as an important platform for the production of various heterologous proteins in recent years. The strong...
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Overexpression and Characterization of Endo-1,4-β-xylanase from Fibrobacter succinogenes in Pichia pastoris
AbstractFibrobacter succinogen es is a major cellulolytic anaerobic bacterium in the gut of herbivores and possesses genes with a wide range of...
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Medium optimization for enhanced production of recombinant lignin peroxidase in Pichia pastoris
ObjectivesDifferent cultivation conditions and parameters were evaluated to improve the production and secretion of a recombinant Phanerochaete...
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Effect and mechanism of signal peptide and maltose on recombinant type III collagen production in Pichia pastoris
AbstractRecombinant type III collagen plays an important role in cosmetics, wound healing, and tissue engineering. Thus, increasing its production is...
