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Combined use of hydrogen-rich water and enzyme-digested edible bird’s nest improves PMA/LPS-impaired wound healing in human inflammatory gingival tissue equivalents
Gingival wound healing plays a critical role in maintaining oral health. However, this process can be delayed by oxidative stress and excessive...
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Changes in physiological states of Salmonella Typhimurium measured by qPCR with PMA and DyeTox13 Green Azide after pasteurization and UV treatment
Diarrheal diseases caused by Salmonella pose a major threat to public health, and assessment of bacterial viability is critical in determining the...
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Detection of viable and total fungal community in zaopei of Chinese strong-flavor baijiu using PMA combined with qPCR and HTS based on ITS2 region
BackgroundChinese strong-flavor baijiu (CSFB), one of the three major baijiu types, is the most popular baijiu type among consumers in China. A...
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All-trans retinoic acid increases NF-κB activity in PMA-stimulated THP-1 cells upon unmethylated CpG challenge by enhancing cell surface TLR9 expression
An active metabolite of vitamin A, all-trans retinoic acid (ATRA), is known to exert immunomodulatory functions. This study investigates the possible...
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Arginase from Priestia megaterium and the Effects of CMCS Conjugation on Its Enzymological Properties
Arginase has shown promising potential in treating cancers by arginine deprivation therapy; however, low enzymatic activity and stability of arginase...
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Targeting c-Myc with decoy oligodeoxynucleotide-loaded polycationic nanoparticles inhibits cell growth and induces apoptosis in cancer stem-like cells (NTERA-2)
BackgroundAn increase in cancer stem cell (CSC) populations and their resistance to common treatments could be a result of c-Myc...
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Uncovering Viable Microbiome in Anaerobic Sludge Digesters by Propidium Monoazide (PMA)-PCR
Use of anaerobic sludge digester is a common practice around the world for solids digestion and methane generation from municipal sewage sludge....
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Quickly assessing disinfection effectiveness to control the spread of African swine fever virus
AbstractInfectious African swine fever virus (ASFV) can cause the spread and morbidity of African swine fever, while the inactivated virus cannot....
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Hydrogen sulfide improves salt tolerance through persulfidation of PMA1 in Arabidopsis
Key messageA new interaction was found between PMA1 and GRF4. H 2 S promotes the interaction through persulfidated Cys446 of PMA1. H 2 S activates PMA1...
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Simultaneous detection of Salmonella spp., Pseudomonas aeruginosa, Bacillus cereus, and Escherichia coli O157:H7 in environmental water using PMA combined with mPCR
A multiplex polymerase chain reaction (mPCR) with propidium monoazide (PMA) and internal amplification control (IAC) for the simultaneous detection...
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DNA from non-viable bacteria biases diversity estimates in the corals Acropora loripes and Pocillopora acuta
BackgroundNucleic acid-based analytical methods have greatly expanded our understanding of global prokaryotic diversity, yet standard metabarcoding...
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Relic DNA effects on the estimates of bacterial community composition and taxa dynamics in soil
AbstractDNA-based analyses have become routine methods in soil microbial research, for their high throughput and resolution in characterizing...
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Identification of management zones with different potential moisture availability for sustainable intensification of dryland agriculture
In semiarid environments moisture availability is the primary factor that controls land productivity, however, no method to differentiate landscape...
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Involvement of Mitogen-Activated Protein Kinases p38 and ERK1/2, as well as Protein Kinase B Akt1/2, in the Formation of Neutrophil Extracellular Traps
AbstractNeutrophils release decondensed nuclear chromatin or neutrophil extracellular trap (NETs) in response to a large number of different...
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A novel method for rapid and sensitive detection of viable Escherichia coli cells using UV-induced PMA-coupled quantitative PCR
We report a specific and sensitive method to improve the coupling of propidium monoazide (PMA) with DNA derived from killed cells of Escherichia coli ...
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A comparative characterisation of commercially available lipid-polymer nanoparticles formed from model membranes
From the discovery of the first membrane-interacting polymer, styrene maleic-acid (SMA), there has been a rapid development of membrane solubilising...
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The effect of local anaesthetics on apoptosis and NETosis of human neutrophils in vitro: comparison between lidocaine and ropivacaine
There are immunological consequences to the method by which neutrophils undergo cell death. Neutrophil apoptosis, called silent death, leads to the...
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Formononetin ameliorates the LPS-induced inflammatory response and apoptosis of neuronal cells via NF-κB/NLRP3 signaling pathway
The objective of this study was to investigate the impact of formononetin on cellular apoptosis and inflammatory responses following spinal cord...
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Increasing HbA1c is associated with reduced CD8+ T cell functionality in response to influenza virus in a TCR-dependent manner in individuals with diabetes mellitus
Diabetes mellitus is on the rise globally and is a known susceptibility factor for severe influenza virus infections. However, the mechanisms by...
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Lipofundin mediates major inhibition of intravenous propofol on phorbol myristate acetate and Escherichia coli-induced neutrophil extracellular traps
BackgroundNeutrophil extracellular traps (NETs) consist of chromatin DNA networks that are studded with cytosolic and granular antimicrobial proteins...
