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Application of OpenArray RT-qPCR for identification of microRNA expression signatures of lower extremity artery disease
Lower extremity artery disease (LEAD) is an underestimated chronic vascular disease caused by the formation of atherosclerotic plaques in the lower...
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QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genoty** and Gene Expression Analysis
Real time technology provides great advancements over PCR-based methods for a broad range of applications. With the increased availability of... -
A Simple Method for Profiling and Analyzing MicroRNAs from Small Volume Samples Using a qPCR-Based Platform
MicroRNAs are small molecules of non-coding RNAs involved in the regulation of mRNA expression, generally through inhibition of translation. Major... -
Validation of SNP markers for fruit quality and disease resistance loci in apple (Malus × domestica Borkh.) using the OpenArray® platform
Genome map** has promised much to tree fruit breeding during the last 10 years. Nevertheless, one of the greatest challenges remaining to tree...
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Assessment of Mucin-Associated Gene Expression Levels on the Ocular Surface
The ocular surface is covered with a mucus layer. The mucin-associated genes expressed in the ocular surface cells include MUC1, MUC4, MUC5AC, and... -
Current status of the analytical validation of next generation sequencing applications for pharmacogenetic profiling
BackgroundAnalytical validity is a prerequisite to use a next generation sequencing (NGS)-based application as an in vitro diagnostic test or a...
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A little SNP of this, a little SNP of that: The discovery of 116 single nucleotide polymorphism markers to enable the rapid identification of individual Pacific walrus (Odobenus rosmarus divergens)
We developed single nucleotide polymorphism (SNP) markers to support a genetics-based capture-mark-recapture (CMR) project implemented for the...
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Innovating transcriptomics for practitioners in freshwater fish management and conservation: best practices across diverse resource-sector users
Thriving freshwater fish populations contribute to people’s economic prosperity and wellbeing. Yet, freshwater fish populations are in critical...
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Molecular Characterization and Map** of Stress Resistance Genes Using SNP Platform in Legumes
Legumes play a vital role in agriculture and food security. Biotic and abiotic stresses are major hurdles for legume production and lower the current... -
Multifactor dimensionality reduction reveals a strong gene–gene interaction between STC1 and COL11A1 genes as a possible risk factor of knee osteoarthritis
Articular cartilage is an avascular tissue with a structure that allows it to support and cushion the overload of the surfaces in contact. It...
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Integration of Emerging Genomic Tools in Wheat Improvement
Conventional methods of wheat breeding have contributed immensely to development and release of improved wheat varieties throughout the globe. In... -
Effect of antimicrobial administration on fecal microbiota of critically ill dogs: dynamics of antimicrobial resistance over time
BackgroundMultidrug resistance in companion animals poses significant risks to animal and human health. Prolonged antimicrobial drug (AMD) treatment...
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Recent Advances in the Use of Molecular Markers for Fruit Crop Improvement
In the recent years, DNA markers have emerged as an indispensable tool for fruit crop improvement for the detection and utilization of DNA... -
Tools in Pharmacogenomics Biomarker Identification for Cancer Patients
The understanding of the biological differences which underlie the inter-individual variability in drug response improved the efficacy of cancer... -
Loci associated with variation in gene expression and growth in juvenile salmon are influenced by the presence of a growth hormone transgene
BackgroundGrowth regulation is a complex process influenced by genetic and environmental factors. We examined differences between growth hormone (GH)...
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Unlocking the potential of a validated single nucleotide polymorphism array for genomic monitoring of trade in cheetahs (Acinonyx jubatus)
Cheetahs ( Acinonyx jubatus ) are listed as vulnerable on the International Union for Conservation of Nature Red List of Threatened Species. Threats...
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Digital PCR as a Highly Sensitive Diagnostic Tool: A Review
Abstract —Digital PCR (dPCR) is a nucleic acid quantification method that is widely used in genetic analysis. One of the most significant advantages...
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Haplotype structure of MSTN, IGF1, and BMP2 genes in Tunisian goats (Capra hircus) and their association with morphometric traits
The aim of the present study was to evaluate variability and haplotype structure of twenty-eight single nucleotide polymorphisms (SNPs) at myostatin (
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Molecular Approaches in Restoration of Agro-Biodiversity
Agro-biodiversity is a prerequisite for the sustainability of any agroecosystem. Loss of agro-biodiversity is a serious global threat today. Numerous... -
Comparing preprocessing strategies for 3D-Gene microarray data of extracellular vesicle-derived miRNAs
BackgroundExtracellular vesicle-derived (EV)-miRNAs have potential to serve as biomarkers for the diagnosis of various diseases. miRNA microarrays...