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Serine O-acetyltransferase derived NV14 peptide reduces cytotoxicity in H2O2 induced MDCK cells and inhibits MCF-7 cell proliferation through caspase gene expression
BackgroundMost of the bioactive peptides exhibit antioxidant effect and do elicit inhibitory effect on proliferation of cancer cells. This study...
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Protective effect of morin by targeting mitochondrial reactive oxygen species induced by hydrogen peroxide demonstrated at a molecular level in MDCK epithelial cells
BackgroundThe development of diabetic nephropathy is aided by the presence of oxidative stress. Morin, a natural flavonoid molecule, has been shown...
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High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells
AbstractSimilar to the recent COVID-19 pandemic, influenza A virus poses a constant threat to the global community. For the treatment of flu disease,...
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Tracking changes in adaptation to suspension growth for MDCK cells: cell growth correlates with levels of metabolites, enzymes and proteins
AbstractAdaptations of animal cells to growth in suspension culture concern in particular viral vaccine production, where very specific aspects of...
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Methods for Establishing Rab Knockout MDCK Cells
The Rab family small GTPases are key regulators of intracellular membrane traffic that are conserved in all eukaryotic cells. Rabs are thought to...
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The Use of Three-Dimensional Cell Culture to Study Apicobasal Polarization and Lumen Formation
The three-dimensional culture of epithelial cells allows the characterization of processes required for collective epithelial polarization, such as...
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Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine
AbstractN -linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in...
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Force-Dependent Remodeling of Cell-to-Cell Adhesion Through the Regulation of Phase Separation
During development and regeneration, cells vigorously migrate to achieve morphogenesis of tissues and organs. Upon these dynamic processes, cells are...
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A human cell polarity protein Lgl2 regulates influenza A virus nucleoprotein exportation from nucleus in MDCK cells
In this study, the regulatory effect of the overexpression of polarity protein Lgl2 on the nuclear export of influenza A virus nucleoprotein in...
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Overview to Animal Cell Culture
The technique of taking cells from an animal or plant and growing them in an artificially controlled environment is known as cell culture. In many...
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Applications of Animal Cell Culture-Based Assays
Advances in animal cell culture and cell-based assays have revolutionised the current scenario for development of biomarkers, genetic manipulation...
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JAM-A interacts with α3β1 integrin and tetraspanins CD151 and CD9 to regulate collective cell migration of polarized epithelial cells
Junctional adhesion molecule (JAM)-A is a cell adhesion receptor localized at epithelial cell–cell contacts with enrichment at the tight junctions....
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Media and Buffer Preparation for Cell Culture
Cell culture is the most widely studied method in the field of life sciences. This term is generally used for the removal of cells, tissues or organs...
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Vectorial transport of the arginine derivatives asymmetric dimethylarginine (ADMA) and l-homoarginine by OATP4C1 and P-glycoprotein studied in double-transfected MDCK cells
Elevated plasma concentrations of the uremic toxin asymmetric dimethylarginine (ADMA) and low plasma concentrations of l -homoarginine are...
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INF2 formin variants linked to human inherited kidney disease reprogram the transcriptome, causing mitotic chaos and cell death
Mutations in the human INF2 gene cause autosomal dominant focal segmental glomerulosclerosis (FSGS)—a condition characterized by podocyte loss,...
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PATJ inhibits histone deacetylase 7 to control tight junction formation and cell polarity
The conserved multiple PDZ-domain containing protein PATJ stabilizes the Crumbs-Pals1 complex to regulate apical-basal polarity and tight junction...
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Three new nonenes from culture broth of marine-derived fungus Albifimbria verrucaria and their cytotoxic and anti-viral activities
Three new nonenes, verrucanonenes A‒C ( 1 ‒ 3 ), were isolated from culture broth of marine-derived fungus Albifimbria verrucaria . These compounds were...
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To not love thy neighbor: mechanisms of cell competition in stem cells and beyond
Cell competition describes the process in which cells of greater fitness are capable of sensing and instructing elimination of lesser fit mutant...
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Need for standardization of Influenza A virus-induced cell death in vivo to improve consistency of inter-laboratory research findings
The involvement of necroptosis in the control of influenza A virus (IAV) infection has been reported in multiple studies. Downstream of the nucleic...
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Identification and differential expression of microRNAs in Madin–Darby canine kidney cells with high and low tumorigenicities
BackgroundMadin–Darby canine kidney (MDCK) cells are widely used for vaccine production, however, the safety of MDCK cells needs to be considered...
