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Multiplex gene editing and large DNA fragment deletion by the CRISPR/Cpf1-RecE/T system in Corynebacterium glutamicum
Corynebacterium glutamicum is an essential industrial strain that has been widely harnessed for the production of all kinds of value-added products....
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The male sterility of bread wheat line XN291S was resulted from the deletion of a large segment at the end of 6BS
The sterile lines used in the production of two-line hybrid wheat in China are mainly photoperiod-thermo sensitive male sterile (PTSMS), and it is of...
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CRISPR-Cas9-mediated deletion enhancer of MECOM play a tumor suppressor role in ovarian cancer
MDS1 and EVI1 complex locus (MECOM), a transcription factor encoding several variants, has been implicated in progression of ovarian cancer. The...
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Deletion and replacement of long genomic sequences using prime editing
Genomic insertions, duplications and insertion/deletions (indels), which account for ~14% of human pathogenic mutations, cannot be accurately or...
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Sensing chemical-induced DNA damage using CRISPR/Cas9-mediated gene-deletion yeast-reporter strains
AbstractMicroorganism-based genotoxicity assessments are vital for evaluating potential chemical-induced DNA damage. In this study, we developed both...
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Deletion of gene OV132 attenuates Orf virus more effectively than gene OV112
AbstractOrf virus (ORFV), a Parapoxvirus in Poxviridae , infects sheep and goats resulting in contagious pustular dermatitis. ORFV is regarded as a...
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Seedless fruit in Annona squamosa L. is monogenic and conferred by INO locus deletion in multiple accessions
Key messageInheritance of the presence/absence of seeds in Annona squamosa is mediated by a single fully recessive gene and is caused by a deletion...
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Generation of Deletion Lines in Allohexaploid Bread Wheat
Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its large genome, high density of repeats, low...
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Ancestry of the major long-range regulatory site of the α-globin genes in the Portuguese population with the common 3.7 kb α-thalassemia deletion
BackgroundThe α-Major Regulatory Element (α-MRE), also known as HS-40, is located upstream of the α-globin gene cluster and has a crucial role in the...
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Activation of secondary metabolite gene clusters in Chaetomium olivaceum via the deletion of a histone deacetylase
AbstractHistone acetylation modifications in filamentous fungi play a crucial role in epigenetic gene regulation and are closely linked to the...
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Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering
AbstractBacteria are versatile living systems that enhance our understanding of nature and enable biosynthesis of valuable chemicals. Long fragment...
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Identification and characterization of the largest deletion in the PCCA gene causing severe acute early-onset form of propionic acidemia
Whole-exome sequencing (WES) is an excellent method for the diagnosis of diseases of uncertain or heterogeneous genetic origin. However, it has...
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Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two
BackgroundIn plant genome editing, RNA-guided nucleases such as Cas9 from Streptococcus pyogenes ( Sp Cas9) predominantly induce small insertions or...
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Deletion of ARGLU1 causes global defects in alternative splicing in vivo and mouse cortical malformations primarily via apoptosis
Haploinsufficient mutation in arginine and glutamine-rich protein 1 ( Arglu1 ), a newly identified pre-mRNA splicing regulator, may be linked to neural...
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Chimeric deletion mutation of rpoC2 underlies the leaf-patterning of Clivia miniata var. variegata
Key messageThe deletion mutated rpoC2 leads to yellow stripes of Clivia miniata var. variegata by down regulating the transcription of 28...
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Deletion of Rap-phosphatases for quorum sensing control in Bacillus and its effect on surfactin production
The complex regulatory network in Bacillus , known as quorum sensing, offers many opportunities to modify bacterial gene expression and hence to...
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Off-target activity of the 8 kb Dmp1-Cre results in the deletion of Tsc1 gene in mouse intestinal mesenchyme
The Dmp1-Cre mouse, expressing Cre from an 8-kb DNA fragment of the mouse Dmp1 gene, is a common tool to study gene functions in osteocytes. Here we...
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A 4.43-Kb deletion of chromosomal segment containing an ovate family protein confers long capsule in sesame (Sesamum indicum L.)
Key messageA 4.43-Kb structural variation in the sesame genome results in the deletion of the Siofp1 gene and induces the long capsule length trait.
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Rapid visual genoty** method for germline mutants with small genomic fragment deletion by allele-specific PCR and lateral flow nucleic acid biosensor
BackgroundGenome-editing techniques incorporating artificial nucleases develop rapidly and enable efficient and precise modification of genomic DNA...
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Proansamycin B derivatives from the post-PKS modification gene deletion mutant of Amycolatopsis mediterranei S699
Ten new proansamycin B congeners ( 1 – 10 ) together with one known ( 11 ) were isolated and characterized on the basis of 1D and 2D NMR spectroscopic and...
