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Scratching the surface: The in vitro research that will be critical for conserving exceptional plants to scale
The conservation of threatened exceptional plants, which cannot be conserved by seed banking, requires in vitro technologies for many of the...
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In Vitro Porcine (Explant) Colon Culture
Models have been extensively used to investigate disease pathogenesis. Animal models are costly and require extensive logistics for animal care, and...
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Functional characterization of Labeo rohita muscle cell line for in vitro research
BackgroundLabeo rohita represents the most dominant fish species in Indian aquaculture and the fish cell lines have been used as an excellent in...
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Three-dimensional in vitro culture models in oncology research
Cancer is a multifactorial disease that is responsible for 10 million deaths per year. The intra- and inter-heterogeneity of malignant tumors make it...
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In Vitro Production of Arbuscular Mycorrhizal Fungi: An Overview
The arbuscular mycorrhizal fungi (AMF) forms root association with majority of land plants and both the partner get benefits from each other in...
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Role and relevance of fish cell lines in advanced in vitro research
IntroductionCell line derived from fish has been established as a promising tool for studying many key issues of aquaculture covering fish growth,...
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In Vitro Culture of White Adipose Tissue
White adipose tissue (WAT) plays a crucial endocrine organ that regulates blood glucose and lipid levels, satiety, and inflammation. Before the...
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New Insights for the Production of Medicinal Plant Materials: Ex Vitro and in Vitro Propagation
Medicinal plants are a vital input of raw materials for the manufacture of pharmaceuticals to treat various ailments of humans and animals. The...
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In Vitro Culture of Arabidopsis Embryos
Arabidopsis thaliana is currently the most important model organism for basic molecular plant research. It is also a favourable model for...
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In Vitro Biotechnological Progressions in Pueraria tuberosa (Willd.) DC.
Pueraria tuberosa (Willd.) DC., belonging to the family Fabaceae, is in great demand in Ayurveda because of its healing properties against weakness,...
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Acute vs. chronic vs. intermittent hypoxia in breast Cancer: a review on its application in in vitro research
Hypoxia has been linked to elevated instances of therapeutic resistance in breast cancer. The exposure of proliferating cancer cells to hypoxia has...
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Transplant and Care for In Vitro Orchid Seedlings
Transferring young orchid seedlings from in vitro culture to the external environment is critical for orchid micropropagation. This chapter...
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Large-Scale Production of Specialized Metabolites In Vitro Cultures
For centuries plants have been intensively utilized as reliable sources of food, flavoring, and pharmaceutical ingredients. However, plant natural...
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In Vitro Cytotoxicity Analysis: MTT/XTT, Trypan Blue Exclusion
In vitro cytotoxicity analyses have played a major role in the current drug discovery processes. These analyses enable the measurement of target cell...
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Models for calcific aortic valve disease in vivo and in vitro
Calcific Aortic Valve Disease (CAVD) is prevalent among the elderly as the most common valvular heart disease. Currently, no pharmaceutical...
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Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells (SSCs)
Preservation of human spermatogonial stem cells (SSCs) may be suitable for young male patients at risk of male infertility due to various causes,...
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In Vitro Propagation of Plants via Organogenesis in Bambusa vulgaris Schrad. ex Wendl Using Temporary Immersion Systems
The low multiplication and ex vitro survival rates during acclimatization in the culture house limit the in vitro mass propagation of B. vulgaris....
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In Vitro Gamma Mutagenesis Techniques in Rice (Oryza sativa L. var. Lazarroz FL)
Gamma radiation (60Co)-induced mutagenesis offers an alternative to develop rice lines by accelerating the spontaneous mutation process and...
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Neutral Red Uptake Assay to Assess Cytotoxicity In Vitro
The neutral red uptake (NRU) assay is a cell viability assay that can be used for the assessment of compound-induced cytotoxicity. It is based on the...
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In Vitro Amplification and Selection of Engineered RNase P Ribozyme for Gene Targeting Applications
Ribozymes engineered from the RNase P catalytic RNA (M1 RNA) represent promising gene-targeting agents for clinical applications. We describe in this...