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Signal Transduction Immunohistochemistry Methods and Protocols
This new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers develo** novel...
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In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue
Detection of phosphorylated proteins in tissue sections using immunohistochemistry (IHC) is a challenging task. The absence of tissue staining may be...
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Immunohistochemistry
Immunohistochemistry (IHC) is the technique of assessing the expressed protein in vivo through antigen-antibody reaction. In this technique, a more...
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Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence
Ferroptosis is a type of regulated cell death driven by oxidative damage, characterized by iron overload and lipid peroxidation, and regulated by a...
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Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry
Technologies for staining and imaging multiple antigens in single tissue sections are develo** rapidly due to their potential to uncover spatial...
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MYCN immunohistochemistry as surrogate marker for MYCN-amplified spinal ependymomas
MYCN (master regulator of cell cycle entry and proliferative metabolism) gene amplification defines a molecular subgroup of spinal cord ependymomas...
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Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ
Macrophages contribute to many aspects of development and homeostasis, innate and acquired immunity, immunopathology, and tissue repair. Every tissue...
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A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol
BackgroundIn the past few years, there has been an explosion in single-cell transcriptomics datasets, yet in vivo confirmation of these datasets is...
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Immunohistochemistry: A Method to Analyze M. ulcerans Infected Skin Tissue
Immunohistochemistry (IHC) is a combination of immunological, biochemical, and pathological methods to visualize the presence and distribution of...
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Validating Antibody Specificities for Immunohistochemistry by Protein Blotting
Optimized antibody reagents are important in research, and erratic antibody performance leads to variability in immunoassays. Specificity of...
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Recent Advances in Chromogens for Immunohistochemistry
Various staining strategies and color combinations have been developed to perform single and double immunohistochemical staining on biological...
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Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry
Multiplex immunohistochemistry (mIHC) facilitates the simultaneous detection of various immune cell markers on a single tissue section. Here, we...
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Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to Phenotype Glial Cells in Formalin-Fixed Paraffin-Embedded Human Brain Sections
There is a growing interest in expanding the multiplexing capability of immunohistochemistry to achieve a deeper phenoty** of various cell types in...
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Supported Immunohistochemical Staining of Mucins
Immunohistochemistry (IHC) staining is the most common method to detect the distribution and localization of biomarkers in different parts of a...
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Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species
Chromogenic immunohistochemistry (IHC) serves as an essential assay for the diagnoses of many diseases including cancer. Single-marker IHC detection...
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Selection of optimal quantile protein biomarkers based on cell-level immunohistochemistry data
BackgroundProtein biomarkers of cancer progression and response to therapy are increasingly important for improving personalized medicine. Advanced...
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Immunohistochemistry of Human Hsp60 in Health and Disease: Recent Advances in Immunomorphology and Methods for Assessing the Chaperonin in Extracellular Vesicles
The subject matter of this chapter is defined by the title of its two previous editions, “Immunohistochemistry of human Hsp60 in health and disease:...
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Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men
Germ cell tumors (GCT) in men comprise of tumor subtypes with distinct histomorphologies, genetic and genomic alterations, and clinical behavior....
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Combined Lectin- and Immuno-histochemistry (CLIH) for Fluorescence Microscopy
The function of glycoproteins depends both on their polypeptide chain and sugar residues. For detection and localization of glycoproteins in tissue...