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An improved and extended dual-index multiplexed 16S rRNA sequencing for the Illumina HiSeq and MiSeq platform
BackgroundRecent advancements in next-generation sequencing (NGS) technology have ushered in significant improvements in sequencing speed and data...
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High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’ (0–10) spacer-linked target specific primers without PhiX spike-in
BackgroundIllumina sequencing platform requires base diversity in the initial 11 cycles for efficient cluster identification and colour matrix...
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Targeted Sequencing of Circular RNAs for Illumina-Based Counting and Nanopore Structure Determination
In the past years, circular RNAs (circRNAs) became a major focus of many studies in animals and plants. circRNAs are generated by backsplicing from...
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Comparison of the Illumina NextSeq 2000 and GeneMind Genolab M sequencing platforms for spatial transcriptomics
BackgroundThe Illumina sequencing systems demonstrate high efficiency and power and remain the most popular platforms. Platforms with similar...
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Comparison of capture-based mtDNA sequencing performance between MGI and illumina sequencing platforms in various sample types
BackgroundMitochondrial genome abnormalities can lead to mitochondrial dysfunction, which in turn affects cellular biology and is closely associated...
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Boosting variant-calling performance with multi-platform sequencing data using Clair3-MP
BackgroundWith the continuous advances in third-generation sequencing technology and the increasing affordability of next-generation sequencing...
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The effect of library preparation protocol on the efficiency of heteroplasmy detection in mitochondrial DNA using two massively parallel sequencing Illumina systems
Massively parallel sequencing (MPS) technology has become the gold standard in mitochondrial DNA research due to its high sensitivity in detecting...
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COSAP: Comparative Sequencing Analysis Platform
BackgroundRecent improvements in sequencing technologies enabled detailed profiling of genomic features. These technologies mostly rely on short...
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Single-Molecule Poly(A) Tail Sequencing (SM-PATseq) Using the PacBio Platform
The polyadenylation of the 3′ ends of messenger RNAs is an important regulator of stability and translation. We developed the single-molecule poly(A)...
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Accuracy benchmark of the GeneMind GenoLab M sequencing platform for WGS and WES analysis
BackgroundGenoLab M is a recently developed next-generation sequencing (NGS) platform from GeneMind Biosciences. To establish the performance of...
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Comparative transcriptome analysis of dioecious floral development in Trachycarpus fortunei using Illumina and PacBio SMRT sequencing
BackgroundTrachycarpus fortunei is a plant with significant economic and ornamental value. Both male and female flowers of T. fortunei originate as...
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Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeq
BackgroundOver 4 million SARS-CoV-2 genomes have been sequenced globally in the past 2 years. This has been crucial in elucidating transmission...
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Application of Deep Sequencing in Phage Display
This chapter describes the workflow to implement deep sequencing into standard phage display experiments on protein libraries. By harvesting the...
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Genome assembly and microsatellite marker development using Illumina and PacBio sequencing in Persicaria maackiana (Polygonaceae) from Korea
BackgroundPersicaria maackiana (Regel) is a potential medicinal plant that exerts anti-diabetic effects. However, the lack of genomic information on P...
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Comparison between MGI and Illumina sequencing platforms for whole genome sequencing
BackgroundIllumina next generation sequencing (NGS) systems are the major sequencing platform in worldwide next-generation sequencing market. On the...
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Combined use of Oxford Nanopore and Illumina sequencing yields insights into soybean structural variation biology
BackgroundStructural variants (SVs), including deletions, insertions, duplications, and inversions, are relatively long genomic variations implicated...
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The Illumina Sequencing Protocol and the NovaSeq 6000 System
The NovaSeq 6000 is a sequencing platform from Illumina that enables the sequencing of short reads with an output up to 6 Tb. The NovaSeq 6000 uses...
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Next Generation Sequencing of DNA-Encoded Libraries
DEL technology is dependent on the generation and analysis of large amounts of DNA sequence data. In this chapter, we describe a method to sequence...
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Next-Generation Sequencing
Next-generation sequencing (NGS) technologies refer to the high-throughput sequencing techniques. “Next-generation” sequencing technologies lead to...
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Next-Generation Sequencing: ForenSeq™ DNA Signature Prep Kit with the Illumina MiSeq FGx
Sequencing forensic DNA samples that are amplified and prepared with the ForenSeq™ DNA Signature Prep Kit allows for the simultaneous targeting of...