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Revisiting biocrystallization: purine crystalline inclusions are widespread in eukaryotes
Despite the widespread occurrence of intracellular crystalline inclusions in unicellular eukaryotes, scant attention has been paid to their...
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Purine salvage–associated metabolites as biomarkers for early diagnosis of esophageal squamous cell carcinoma: a diagnostic model–based study
Esophageal squamous cell carcinoma (ESCC) remains an important health concern in develo** countries. Patients with advanced ESCC have a poor...
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HGprt deficiency disrupts dopaminergic circuit development in a genetic mouse model of Lesch–Nyhan disease
In Lesch–Nyhan disease (LND), deficiency of the purine salvage enzyme hypoxanthine guanine phosphoribosyl transferase (HGprt) leads to a...
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Hybridoma Technology
Monoclonal antibodies are produced by hybridoma technology. The term hybridoma is applied to fused cells resulting due to fusion of lymphocytes and...
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Assessment of reference genes for qRT-PCR normalization to elucidate host response to African swine fever infection
Viral infection disrupts the normal regulation of the host gene’s expression. In order to normalise the expression of dysregulated host genes upon...
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Formate overflow drives toxic folate trap** in MTHFD1 inhibited cancer cells
Cancer cells fuel their increased need for nucleotide supply by upregulating one-carbon (1C) metabolism, including the enzymes...
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Metabolic discrimination of synovial fluid between rheumatoid arthritis and osteoarthritis using gas chromatography/time-of-flight mass spectrometry
IntroductionRheumatoid arthritis (RA) and osteoarthritis (OA) are clinicopathologically different.
ObjectivesWe aimed to assess the feasibility of...
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Nucleotide Biosynthesis and Regulation
Bacteria are fast-growing organisms that need to rapidly synthesize nucleotides to sustain growth. This is true for cancer cells as well. Thus,...
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Genetically modified mice as a tool for the study of human diseases
Modeling a human disease is an essential part of biomedical research. The recent advances in the field of molecular genetics made it possible to...
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Screening of reference genes in tiger puffer (Takifugu rubripes) across tissues and under different nutritional conditions
The present study was aimed at screening suitable reference genes for quantitative real-time polymerase chain reaction (qRT-PCR) in tiger puffer ( Taki...
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IF1 ablation prevents ATP synthase oligomerization, enhances mitochondrial ATP turnover and promotes an adenosine-mediated pro-inflammatory phenotype
ATPase Inhibitory Factor 1 (IF1) regulates the activity of mitochondrial ATP synthase. The expression of IF1 in differentiated human and mouse cells...
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Antibody Isolation in C. neoformans
The importance of humoral immunity to fungal infections remains to be elucidated. In cryptococcosis, patients that fail to generate antibodies...
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Effect of Glucose on Water Transport in Rat Peritoneal Mesothelial Cells
AbstractGlucose is widely used as an osmotic agent in fluids for peritoneal dialysis. The aim of this work was to study the effect of glucose on the...
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Selection of reference genes in liproxstatin-1-treated K562 Leukemia cells via RT-qPCR and RNA sequencing
BackgroundReverse transcription quantitative polymerase chain reaction (RT-qPCR) can accurately detect relative gene expression levels in biological...
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New biomarkers underlying acetic acid tolerance in the probiotic yeast Saccharomyces cerevisiae var. boulardii
AbstractEvolutionary engineering experiments, in combination with omics technologies, revealed genetic markers underpinning the molecular mechanisms...
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Bacillus subtilis supernatant improves the efficacy of radiation therapy in rat intestinal epithelial cells by upregulation of bax and caspase-3 genes
BackgroundColorectal Cancer (CC) is among the most prevalent cancers in elderly persons. Radiotherapy is usually prescribed as CC develops, however,...
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RPS13, a potential universal reference gene for normalisation of gene expression in multiple human normal and cancer tissue samples
BackgroundReference genes are considered stable genes and are used for normalizing the gene expression profile across different cell types; as well...
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Assessment of the drugability of initial malaria infection through miniaturized sporozoite assays and high-throughput screening
The sporozoite stages of malaria parasites are the primary cause of infection of the vertebrate host and are targeted by (experimental) vaccines....
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Culture methods focusing on bile canalicular formation using primary human hepatocytes in a short time
The development of models for predicting hepatotoxicity is warranted, as the hepatotoxicity risk of 38–51% of compounds is undetectable in...
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High-fat diet enhances cell proliferation and compromises intestinal permeability in a translational canine intestinal organoid model
BackgroundEmerging evidence underscores the responsiveness of the mammalian intestine to dietary cues, notably through the involvement of...
