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Precise in vivo RNA base editing with a wobble-enhanced circular CLUSTER guide RNA
Recruiting the endogenous editing enzyme adenosine deaminase acting on RNA (ADAR) with tailored guide RNAs for adenosine-to-inosine (A-to-I) RNA base...
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CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato
CRISPR/Cas9 technology has become the most efficient method for genome editing in many plant species, including important industrial crops such as...
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Mechanisms and functions of RNA-guided RNA modification
RNA-guided 2’-O-methylations and pseudouridylations occur in several different types of RNAs and in a wide range of organisms. Hundreds of the RNAs...
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Directed mutagenesis in plants through genome editing using guide RNA library
Plants with genetic diversity have a higher rate of resilience and adaptation to environmental change. Crop species need to diversify genetically if...
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Engineered circular guide RNAs boost CRISPR/Cas12a- and CRISPR/Cas13d-based DNA and RNA editing
BackgroundThe CRISPR/Cas12a and CRISPR/Cas13d systems are widely used for fundamental research and hold great potential for future clinical...
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Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme
BackgroundCRISPR-Cas13 is a newly emerging RNA knockdown technology that is comparable to RNAi. Among all members of Cas13, CasRx degrades RNA in...
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A Guide to Trajectory Inference and RNA Velocity
Technological developments have led to an explosion of high-throughput single-cell data, which are revealing unprecedented perspectives on cell...
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A Guide to Computational Cotranscriptional Folding Featuring the SRP RNA
Although RNA molecules are synthesized via transcription, little is known about the general impact of cotranscriptional folding in vivo. We present...
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CASowary: CRISPR-Cas13 guide RNA predictor for transcript depletion
BackgroundRecent discovery of the gene editing system - CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) associated proteins...
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Chimeric DNA–RNA Guide RNA Designs
CRISPR-associated nuclease (Cas) has been widely applied to modify the genomes of various cell types. As RNA-guided endonucleases, Cas enzymes can...
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Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA
BackgroundCRISPR-based screens are revolutionizing drug discovery as tools to identify genes whose ablation induces a phenotype of interest. For...
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A streamlined guide RNA screening system for genome editing in Sorghum bicolor
BackgroundGenome editing tools derived from clustered regularly interspaced short palindromic repeats (CRISPR) systems have been developed for...
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A Workflow Guide to RNA-Seq Analysis of Chaperone Function and Beyond
RNA sequencing (RNA-seq) is a powerful method of transcriptional analysis that allows for the sequence identification and quantification of cellular...
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Efficient in vitro and in vivo RNA editing via recruitment of endogenous ADARs using circular guide RNAs
Recruiting endogenous adenosine deaminases using exogenous guide RNAs to edit cellular RNAs is a promising therapeutic strategy, but editing...
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Short-Read RNA-Seq
RNA sequencing (RNA-Seq) has emerged as a powerful and versatile tool for the comprehensive analysis of transcriptomes and has been widely used to...
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Compact CRISPR genetic screens enabled by improved guide RNA library cloning
CRISPR genome editing approaches theoretically enable researchers to define the function of each human gene in specific cell types, but challenges...
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Complicated target recognition by archaeal box C/D guide RNAs
Box C/D RNAs guide the site-specific formation of 2′- O -methylated nucleotides (Nm) of RNAs in eukaryotes and archaea. Although C/D RNAs have been...
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CRISPR Guide RNA Design Methods and Protocols
This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of...
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RNA-Protein Complexes and Interactions Methods and Protocols
This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or...
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CLUSTER guide RNAs enable precise and efficient RNA editing with endogenous ADAR enzymes in vivo
RNA base editing represents a promising alternative to genome editing. Recent approaches harness the endogenous RNA-editing enzyme adenosine...
