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Fluorescence Intensity-Based eIF2B’s Guanine Nucleotide-Exchange Factor Activity Assay
Guanine nucleotide-exchange factors (GEFs) activate the function of guanine nucleotide-binding proteins (G-proteins) by promoting the exchange of GDP...
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Crk proteins activate the Rap1 guanine nucleotide exchange factor C3G by segregated adaptor-dependent and -independent mechanisms
BackgroundC3G is a guanine nucleotide exchange factor (GEF) that activates Rap1 to promote cell adhesion. Resting C3G is autoinhibited and the GEF...
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Guanine Quadruplexes in Cell Nucleus Metabolism
AbstractCell metabolism depends, to a large extent, on correct regulation of gene expression. One of the mechanisms of regulation is the formation of...
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Rho Family GTPases and their Modulators
The Rho family GTPases play an important role in mediating signal transduction in multiple fundamental cellular processes. Most of the Rho family...
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Epiberberine: a potential rumen microbial urease inhibitor to reduce ammonia release screened by targeting UreG
AbstractRumen microbial urease inhibitors have been proposed for regulating nitrogen emission and improving nitrogen utilization efficiency in...
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CSC01 shows promise as a potential inhibitor of the oncogenic G13D mutant of KRAS: an in silico approach
About 30% of malignant tumors include KRAS mutations, which are frequently required for the development and maintenance of malignancies. KRAS is now...
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Quantifying the Binding of Fluorescently Labeled Guanine Nucleotides and Initiator tRNA to Eukaryotic Translation Initiation Factor 2
The translation initiation factor eIF2 is critical for protein synthesis initiation, and its regulation is central to the integrated stress response...
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RhoGDI1 interacts with PHLDA2, suppresses the proliferation, migration, and invasion of trophoblast cells, and participates in the pathogenesis of preeclampsia
Preeclampsia (PE) is a pregnancy-associated disease, which is the major cause of mortality on maternity and perinatal infants. It is hypothesized...
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The Bacterial Gq Signal Transduction Inhibitor FR900359 Impairs Soil-Associated Nematodes
The cyclic depsipeptide FR900359 (FR) is derived from the soil bacterium Chromobacterium vaccinii and known to bind G q proteins of mammals and...
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Imaging of Spatial Cycling of Rab GTPase in the Cell
Rab GTPases (>60 members in human) function as master regulators of intracellular membrane trafficking. To fulfill their functions, Rab proteins need...
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High-Resolution Melting (HRM) Genoty**
High-resolution melting (HRM) analysis is a simple, fast, and inexpensive real-time polymerase chain reaction (PCR)-based method used to identify...
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An Early Season Perspective of Key Differentially Expressed Genes and Single Nucleotide Polymorphisms Involved in Sucrose Accumulation in Sugarcane
Sucrose is the prime product of sugarcane and a significant variation has been observed among different sugarcane genotypes for the time taken to...
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Proteins Cross-talking with Nox Complexes: The Social Life of Noxes
Nox NADPH Oxidases exhibit a basic organization comprising a catalytic transmembrane subunit closely regulated by canonical regulatory subunits,...
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CCDC88B interacts with RASAL3 and ARHGEF2 and regulates dendritic cell function in neuroinflammation and colitis
CCDC88B is a risk factor for several chronic inflammatory diseases in humans and its inactivation causes a migratory defect in DCs in mice. CCDC88B...
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An overview of recent advancements in small molecules suppression of oncogenic signaling of K-RAS: an updated review
RAS (rat sarcoma) oncoproteins are crucial for the growth of some human cancers, including lung, colorectal, and pancreatic adenocarcinomas. The RAS...
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Functions of Rab GTPases in organelle biogenesis
Rab GTPases regulate diverse aspects of the biogenesis of secretory and endocytic organelles. The conformational changes that accompany GTP binding...
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Proteasome Interactome and Its Role in the Mechanisms of Brain Plasticity
AbstractProteasomes are highly conserved multienzyme complexes responsible for proteolytic degradation of the short-lived, regulatory, misfolded, and...
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Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis
Morphological rearrangement of the endoplasmic reticulum (ER) is critical for metazoan mitosis. Yet, how the ER is remodeled by the mitotic signaling...
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The rapid proximity labeling system PhastID identifies ATP6AP1 as an unconventional GEF for Rheb
Rheb is a small G protein that functions as the direct activator of the mechanistic target of rapamycin complex 1 (mTORC1) to coordinate signaling...
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GRK specificity and Gβγ dependency determines the potential of a GPCR for arrestin-biased agonism
G protein-coupled receptors (GPCRs) are mainly regulated by GPCR kinase (GRK) phosphorylation and subsequent β-arrestin recruitment. The ubiquitously...
