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  1. FliH and FliI help FlhA bring strict order to flagellar protein export in Salmonella

    The flagellar type III secretion system (fT3SS) switches substrate specificity from rod-hook-type to filament-type upon hook completion, terminating...

    Miki Kinoshita, Tohru Minamino, ... Keiichi Namba in Communications Biology
    Article Open access 26 March 2024
  2. An overview of the structure and function of the flagellar hook FlgE protein

    The flagellum is an important organelle for the survival of bacteria and consists of a basal body, hook, and filament. The FlgE protein is the...

    Yu You, Fei Ye, ... Shun Deng in World Journal of Microbiology and Biotechnology
    Article 21 March 2023
  3. Flagellar motility of Pseudomonas syringae pv. actinidiae biovar 3 contributes to bacterial infection through stomata

    Highly virulent Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) causes kiwifruit bacterial canker, which seriously damages kiwifruits worldwide....

    Giyu Usuki, Takako Ishiga, ... Yasuhiro Ishiga in Journal of General Plant Pathology
    Article 10 April 2024
  4. Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes

    The complexities of energy transfer mechanisms in the flagella of mammalian sperm flagella have been intensively investigated and demonstrate...

    Huan Wu, Yanman Zhang, ... **ao** He in Science China Life Sciences
    Article 16 May 2024
  5. In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles

    The flagellar axial proteins are transported across the cytoplasmic membrane into the central channel of the growing flagellum via the flagellar...
    Katsumi Imada, Hiroyuki Terashima in Bacterial and Archaeal Motility
    Protocol 2023
  6. The lack of Tex44 causes severe subfertility with flagellar abnormalities in male mice

    By analyzing a mouse Interspecific Recombinant Congenic Strain (IRCS), we previously identified a quantitative trait locus (QTL), called Mafq1 on...

    Sophie Dupuis, Marie-Sophie Girault, ... Sandrine Barbaux in Cellular & Molecular Biology Letters
    Article Open access 15 May 2024
  7. BBOF1 is required for sperm motility and male fertility by stabilizing the flagellar axoneme in mice

    The sperm flagellum is a specialized type of motile cilium composed of a typical “9 + 2” axonemal structure with peri-axonemal structures, such as...

    Huiwen Cao, Haomang Xu, ... Chao Yu in Cellular and Molecular Life Sciences
    Article 17 May 2023
  8. Purification of the Transmembrane Polypeptide Channel Complex of the Salmonella Flagellar Type III Secretion System

    Many motile bacteria employ the flagellar type III secretion system (fT3SS) to build the flagellum on the cell surface. The fT3SS consists of a...
    Miki Kinoshita, Keiichi Namba, Tohru Minamino in Bacterial and Archaeal Motility
    Protocol 2023
  9. The FlhA linker mediates flagellar protein export switching during flagellar assembly

    The flagellar protein export apparatus switches substrate specificity from hook-type to filament-type upon hook assembly completion, thereby...

    Yumi Inoue, Miki Kinoshita, ... Tohru Minamino in Communications Biology
    Article Open access 31 May 2021
  10. Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant

    Salmonella enterica serovar Typhimurium ( S . Typhimurium) is a globally recognized foodborne pathogen that affects both animals and humans....

    Seungmok Han, Ji-Won Byun, Minho Lee in Journal of Microbiology
    Article 05 January 2024
  11. High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Having a Rod-Like Straight Hook

    The bacterial flagellar motor is embedded within the cell envelop and rotates the long helical filament, which acts as a molecular screw to propel...
    Shuichi Nakamura, Tohru Minamino in Bacterial and Archaeal Motility
    Protocol 2023
  12. Purification and CryoEM Image Analysis of the Bacterial Flagellar Filament

    The bacterial flagellum is a large assembly of about 30 different proteins and is divided into three parts: the filament that acts as a screw...
    Tomoko Yamaguchi, Tomoko Miyata, ... Keiichi Namba in Bacterial and Archaeal Motility
    Protocol 2023
  13. DNALI1 deficiency causes male infertility with severe asthenozoospermia in humans and mice by disrupting the assembly of the flagellar inner dynein arms and fibrous sheath

    The axonemal dynein arms (outer (ODA) and inner dynein arms (IDAs)) are multiprotein structures organized by light, intermediate, light intermediate...

    Huan Wu, Yiyuan Liu, ... Yunxia Cao in Cell Death & Disease
    Article Open access 15 February 2023
  14. Molecular Simulation to Investigate Open–Close Motion of a Flagellar Export Apparatus Protein FlhAC

    Molecular dynamics (MD) simulation and parallel cascade selection molecular dynamics (PaCS-MD) are widely used to investigate large-amplitude motions...
    Protocol 2023
  15. Sinorhizobium fredii HH103 flgJ is a flagellar gene induced by genistein in a NodD1- and TtsI- dependent manner

    Background and aims

    Sinorhizobium fredii HH103 is a broad host-range rhizobial strain able to induce the formation of nitrogen-fixing nodules in...

    Pilar Navarro-Gómez, Cynthia Alías-Villegas, ... José-María Vinardell in Plant and Soil
    Article Open access 31 May 2024
  16. The Effect of the flhB Plasmid Gene of the Flagellar Export Component on Flagellation and Motility in Azospirillum Bacteria

    The effect was analyzed of the flhB2 gene, which is located on the AZOBR_p4 (AZOBR_p410073 gene) plasmid in Azospirillum baldaniorum Sp245 and on the...

    L. P. Petrova, I. V. Volokhina, A. V. Sheludko in Molecular Genetics, Microbiology and Virology
    Article 01 September 2023
  17. Genome-wide analysis reveals a rhamnolipid-dependent modulation of flagellar genes in Pseudomonas aeruginosa PAO1

    Pseudomonas aeruginosa is an opportunistic pathogen and an important model organism for the study of bacterial group behaviors, including cell...

    Michele R. Castro, Graciela M. Dias, ... Bianca C. Neves in Current Genetics
    Article 30 January 2022
  18. Flagellar rotational features of an optically confined bacterium at high frequency and temporal resolution reveal the microorganism’s response to changes in the fluid environment

    Rotations of the flagella control the movement of a peritrichous (multiflagellar) bacterium in fluids, the run and tumble events being caused through...

    Ashwini Venkateswara Bhat, Roshan Akbar Basha, ... Sharath Ananthamurthy in European Biophysics Journal
    Article 14 February 2022
  19. Role of the flagellar hook in the structural development and antibiotic tolerance of Pseudomonas aeruginosa biofilms

    Pseudomonas aeruginosa biofilms exhibit an intrinsic resistance to antibiotics and constitute a considerable clinical threat. In cystic fibrosis, a...

    Jules D. P. Valentin, Hervé Straub, ... Qun Ren in The ISME Journal
    Article Open access 08 December 2021
  20. Molecular structure of the intact bacterial flagellar basal body

    The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong,...

    Steven Johnson, Emily J. Furlong, ... Susan M. Lea in Nature Microbiology
    Article 30 April 2021
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