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FliH and FliI help FlhA bring strict order to flagellar protein export in Salmonella
The flagellar type III secretion system (fT3SS) switches substrate specificity from rod-hook-type to filament-type upon hook completion, terminating...
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An overview of the structure and function of the flagellar hook FlgE protein
The flagellum is an important organelle for the survival of bacteria and consists of a basal body, hook, and filament. The FlgE protein is the...
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Flagellar motility of Pseudomonas syringae pv. actinidiae biovar 3 contributes to bacterial infection through stomata
Highly virulent Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) causes kiwifruit bacterial canker, which seriously damages kiwifruits worldwide....
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Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes
The complexities of energy transfer mechanisms in the flagella of mammalian sperm flagella have been intensively investigated and demonstrate...
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In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles
The flagellar axial proteins are transported across the cytoplasmic membrane into the central channel of the growing flagellum via the flagellar... -
The lack of Tex44 causes severe subfertility with flagellar abnormalities in male mice
By analyzing a mouse Interspecific Recombinant Congenic Strain (IRCS), we previously identified a quantitative trait locus (QTL), called Mafq1 on...
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BBOF1 is required for sperm motility and male fertility by stabilizing the flagellar axoneme in mice
The sperm flagellum is a specialized type of motile cilium composed of a typical “9 + 2” axonemal structure with peri-axonemal structures, such as...
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Purification of the Transmembrane Polypeptide Channel Complex of the Salmonella Flagellar Type III Secretion System
Many motile bacteria employ the flagellar type III secretion system (fT3SS) to build the flagellum on the cell surface. The fT3SS consists of a... -
The FlhA linker mediates flagellar protein export switching during flagellar assembly
The flagellar protein export apparatus switches substrate specificity from hook-type to filament-type upon hook assembly completion, thereby...
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Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant
Salmonella enterica serovar Typhimurium ( S . Typhimurium) is a globally recognized foodborne pathogen that affects both animals and humans....
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High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Having a Rod-Like Straight Hook
The bacterial flagellar motor is embedded within the cell envelop and rotates the long helical filament, which acts as a molecular screw to propel... -
Purification and CryoEM Image Analysis of the Bacterial Flagellar Filament
The bacterial flagellum is a large assembly of about 30 different proteins and is divided into three parts: the filament that acts as a screw... -
DNALI1 deficiency causes male infertility with severe asthenozoospermia in humans and mice by disrupting the assembly of the flagellar inner dynein arms and fibrous sheath
The axonemal dynein arms (outer (ODA) and inner dynein arms (IDAs)) are multiprotein structures organized by light, intermediate, light intermediate...
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Molecular Simulation to Investigate Open–Close Motion of a Flagellar Export Apparatus Protein FlhAC
Molecular dynamics (MD) simulation and parallel cascade selection molecular dynamics (PaCS-MD) are widely used to investigate large-amplitude motions... -
Sinorhizobium fredii HH103 flgJ is a flagellar gene induced by genistein in a NodD1- and TtsI- dependent manner
Background and aimsSinorhizobium fredii HH103 is a broad host-range rhizobial strain able to induce the formation of nitrogen-fixing nodules in...
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The Effect of the flhB Plasmid Gene of the Flagellar Export Component on Flagellation and Motility in Azospirillum Bacteria
The effect was analyzed of the flhB2 gene, which is located on the AZOBR_p4 (AZOBR_p410073 gene) plasmid in Azospirillum baldaniorum Sp245 and on the...
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Genome-wide analysis reveals a rhamnolipid-dependent modulation of flagellar genes in Pseudomonas aeruginosa PAO1
Pseudomonas aeruginosa is an opportunistic pathogen and an important model organism for the study of bacterial group behaviors, including cell...
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Flagellar rotational features of an optically confined bacterium at high frequency and temporal resolution reveal the microorganism’s response to changes in the fluid environment
Rotations of the flagella control the movement of a peritrichous (multiflagellar) bacterium in fluids, the run and tumble events being caused through...
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Role of the flagellar hook in the structural development and antibiotic tolerance of Pseudomonas aeruginosa biofilms
Pseudomonas aeruginosa biofilms exhibit an intrinsic resistance to antibiotics and constitute a considerable clinical threat. In cystic fibrosis, a...
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Molecular structure of the intact bacterial flagellar basal body
The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong,...