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Isolated microspore culture for embryoid production in Artemisia annua L.
The haploidy technique is a useful tool for quickly producing pure, fully homozygous lines. Artemisia annua L. is a medicinal plant that produces...
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Embryoid Bodies–Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions
Human embryonic stem cells (hESCs) can differentiate into any cell lineage (pluripotency potential) derived from the three germ layers: ectoderm,...
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Expression profiling of stemness markers in testicular germline stem cells from neonatal and adult Swiss albino mice during their transdifferentiation in vitro
BackgroundSpermatogonial stem cells (SSCs) were considered to be stem cells with limited potencies due to their existence in adult organisms....
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Preliminary Exposure to Histone Deacetylase Inhibitors Changes the Direction of Human iPSCs’ Differentiation with the Formation of Cardiospheres Instead of Skin Organoids
AbstractPluripotent stem cells (PSCs) are a unique cell type that can differentiate into all cell types in the body. In PSC culture, subpopulations...
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Generation of Induced Pluripotent Stem Cell Line iTAF15Xsk4 from Fibroblasts of a Patient with Microdeletion at Xq24
AbstractDifferentiation of induced pluripotent stem cells (iPSCs) from patients and healthy donors allows in vitro study of genetic disorders. The...
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The relationship between regulatory changes in cis and trans and the evolution of gene expression in humans and chimpanzees
BackgroundComparative gene expression studies in apes are fundamentally limited by the challenges associated with sampling across different tissues....
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Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins
BackgroundEver since their discovery, induced pluripotent stem cells (iPSCs) have been extensively differentiated into a large variety of cell types....
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A Modified SMART-Seq Method for Single-Cell Transcriptomic Analysis of Embryoid Body Differentiation
Embryoid bodies (EBs) are aggregate of cells that contain three embryonic germ layers. They can be formed by direct differentiation from pluripotent...
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Trisomy 12 compromises the mesendodermal differentiation propensity of human pluripotent stem cells
Trisomy 12 is one of the most frequent chromosomal abnormalities in cultured human pluripotent stem cells (hPSCs). Although potential oncogenic...
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Transgenic Lines of Human Induced Pluripotent Stem Cells ICGi022-A-6 and ICGi022-A-7 with Doxycycline-Inducible Variants of Programmable Nuclease AsCas12a
AbstractGenome editing in human pluripotent stem cells using programmable nucleases makes it possible to create models of hereditary pathologies...
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Generation of Hepatocyte Organoids from Human iPS Cells
Human-induced pluripotent stem cells (hiPSCs) constitute a great source to generate specialized cells that can be employed in cell replacement...
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Ethical, legal, regulatory, and policy issues concerning embryoids: a systematic review of the literature
Recent advances in methods to culture pluripotent stem cells to model human development have resulted in entities that increasingly have...
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Establishment of Capra hircus somatic cells and induction of pluripotent stem-like cells
Capra hircus (goat) induced pluripotent stem cells (giPSCs) harbor enormous scientific value and contribute to cellular agriculture, animal cloning,...
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The role of c-Jun for beating cardiomycyte formation in prepared embryonic body
BackgroundMorbidity and mortality associated with cardiovascular diseases, such as myocardial infarction, stem from the inability of terminally...
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Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies
FluoVolt, a membrane potential dye, has been used to depict the action potentials of cardiomyocytes derived from human-induced pluripotent stem cells...
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DNA methyltransferases 3A and 3B target specific sequences during mouse gastrulation
In mammalian embryos, DNA methylation is initialized to maximum levels in the epiblast by the de novo DNA methyltransferases DNMT3A and DNMT3B before...
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Exosomes induce neurogenesis of pluripotent P19 cells
Exosomes play a role in tissue/organ development and differentiation. Retinoic acid induces differentiation of P19 cells (UD-P19) to P19 neurons...
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High Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model
The 3D embryoid body (EB) differentiation model is a promising tool for fundamental cell biology and drug discovery studies assessing the compound...
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A Modified Murine Embryonic Stem Cell Test for Evaluating the Teratogenic Effects of Drugs
Animal-based test systems have traditionally been used to screen for the potential teratogenic activity of drugs. Still, their deficits in predicting...
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Production and Analysis of Human Primordial Germ Cell–Like Cells
Primordial germ cells (PGCs) are common ancestors of all germline cells. In mammals, PGCs emerge in early-stage embryos around the timing of...