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The use of EPR spectroscopy to study transcription mechanisms
Electron paramagnetic resonance (EPR) spectroscopy has become a promising structural biology tool to resolve complex and dynamic biological...
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Linewidth, field, and frequency in electron paramagnetic resonance (EPR) spectroscopy
EPR spectroscopy requires a combination of fixed microwave radiation and resonant external magnetic field. Bigger is not necessarily better in that...
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Quantifying the Number and Affinity of Mn2+-Binding Sites with EPR Spectroscopy
During the last decades, various functional oligonucleotides have been discovered including DNAzymes, ribozymes, and riboswitches. Their function is...
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Use of Site-Directed Spin Labeling EPR Spectroscopy to Study Protein–LPS Interactions
Site-directed spin labeling EPR (electron paramagnetic resonance) spectroscopy is a technique used to identify the local conformational changes at a...
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EPR Spectroscopy as a Method for ROS Quantification in the Skin
Electron paramagnetic resonance (EPR) spectroscopy is an established method for the measurement of free radicals. Solar radiation is essential for...
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Using a chimeric respiratory chain and EPR spectroscopy to determine the origin of semiquinone species previously assigned to mitochondrial complex I
BackgroundFor decades, semiquinone intermediates have been suggested to play an essential role in catalysis by one of the most enigmatic...
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Detection of Superoxide Radical in Adherent Living Cells by Electron Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones
Spin trap** with cyclic nitrones coupled to electron paramagnetic resonance (EPR) enables the detection and characterization of oxygen-derived free...
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Application of electron paramagnetic resonance spectroscopy for determining the relative nanoenvironment fluidity of polymeric micelles
AbstractPolymeric micelles are nanocarriers for drug, protein and gene delivery due to their unique core/shell structure, which encapsulates and...
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Cyanide replaces substrate in obligate-ordered addition of nitric oxide to the non-heme mononuclear iron AvMDO active site
Thiol dioxygenases are a subset of non-heme mononuclear iron oxygenases that catalyze the O 2 -dependent oxidation of thiol-bearing substrates to yield...
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Catalytic role of histidine-114 in the hydrolytic dehalogenation of chlorothalonil by Pseudomonas sp. CTN-3
Chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile; TPN) is an environmentally persistent fungicide that sees heavy use in the USA and is highly...
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Human frataxin, the Friedreich ataxia deficient protein, interacts with mitochondrial respiratory chain
Friedreich ataxia (FRDA) is a rare, inherited neurodegenerative disease caused by an expanded GAA repeat in the first intron of the FXN gene, leading...
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Pattern of ROS generation and interconversion on wet stigmas in basal and divergent angiosperms
The active participation of ROS in the reproductive physiology of flowering plants is well-documented in model species, mainly plants with dry...
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Preparation of Site-Specifically Spin-Labeled RNA by in Vitro Transcription Using an Expanded Genetic Alphabet
Recent advances in pulsed electron paramagnetic resonance (EPR) spectroscopy enable studying structure and folding of nucleic acids. An efficient...
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Characterization of Paramagnetic Iron-Sulfur Clusters Using Electron Paramagnetic Resonance Spectroscopy
Continuous-wave (CW) electron paramagnetic resonance (EPR) spectroscopy is a powerful ally in characterizing the multitude of redox-active...
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PELDOR Measurements on Nitroxide-Labeled Oligonucleotides
In the past decades, pulsed dipolar electron paramagnetic resonance spectroscopy (PDS) has emerged as a powerful tool in biophysical chemistry to...
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Mutation of a metal ligand stabilizes the high-spin form of the S2 state in the O2-producing Mn4CaO5 cluster of photosystem II
The residue D1-D170 bridges Mn4 with the Ca ion in the O 2 -evolving Mn 4 CaO 5 cluster of Photosystem II. Recently, the D1-D170E mutation was shown to...
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The Effect of Goethites on the Polymerization of Glycine and Alanine Under Prebiotic Chemistry Conditions
After pre concentration of monomers, polymerization is the second most important step for molecular evolution. The formation of peptides is an...
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Cellular maturation of an iron-type nitrile hydratase interrogated using EPR spectroscopy
AbstractNitrile hydratase (NHase) is a non-heme iron-containing enzyme that has applications in commodity chemical synthesis, pharmaceutical...
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Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy
Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling is ideally suited to study structure, dynamics,...
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Copper(II) cation and bathophenanthroline coordination enhance therapeutic effects of naringenin against lung tumor cells
The development of new anticancer compounds is one of the challenges of bioinorganic and medicinal chemistry. Naringenin and its metal complexes have...
