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Rational design of a disulfide bridge increases the thermostability of microbial transglutaminase
AbstractMicrobial transglutaminase (MTG) has numerous industrial applications in the food and pharmaceutical sectors. Unfortunately, the...
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Improvement of FGF7 Thermal Stability by Introduction of Mutations in Close Vicinity to Disulfide Bond and Surface Salt Bridge
Fibroblast Growth Factor 7 (FGF7), a growth factor specific to epithelial cells, has attracted attention as a therapeutic protein. However, FGF7 has...
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Absence of calcium-sensing receptor basal activity due to inter-subunit disulfide bridges
G protein-coupled receptors naturally oscillate between inactive and active states, often resulting in receptor constitutive activity with important...
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Effect of introducing a disulfide bridge on the thermostability of microbial transglutaminase from Streptomyces mobaraensis
Microbial transglutaminase (MTG) has been used extensively in academic research and the food industry through cross-linking or posttranslational...
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Knotting terminal ends of mutant T1 lipase with disulfide bond improved structure rigidity and stability
AbstractLipase biocatalysts offer unique properties which are often impaired by low thermal and methanol stability. In this study, the rational...
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Optimal Method for Disulfide Bond Closure in the Synthesis of Atosiban—Antagonist of Oxytocin Receptors
AbstractThis work is devoted to the large-scale solid-phase synthesis (SPS) of Atosiban, Mpa 1 -D-Tyr(OEt)-Ile-Thr-Asn-Cys 6 -Pro-Orn-Gly-NH 2 cyclic 1,6...
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Improving D-carbamoylase thermostability through salt bridge engineering for efficient D-p-hydroxyphenylglycine production
D -p -hydroxyphenylglycine (D-HPG) is an important intermediate in the pharmaceutical industry, and it is commonly synthesized by cascading...
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Total Synthesis of Novel Antimicrobial β-Hairpin Capitellacin Via Rapid Flow-Based SPPS Assembly and Regioselective On-Resin Disulfide Cyclisation
Antimicrobial resistance is an ever-increasing global health risk that remains one of the World Health Organisations top priorities. Antimicrobial...
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Protein Disulfide Bonds Detected by Tagging with High Molecular Weight Maleimide Derivative
AbstractDisulfide bridges are essential for maintaining the structure and function of proteins. Traditionally, studies of the disulfide bonds require...
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TRAP1 S-nitrosylation as a model of population-shift mechanism to study the effects of nitric oxide on redox-sensitive oncoproteins
S -nitrosylation is a post-translational modification in which nitric oxide (NO) binds to the thiol group of cysteine, generating an S -nitrosothiol...
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Activation of cell-penetrating peptide fragments by disulfide formation
Three cell-penetrating peptides (CPPs), Tat, Pep-3 and penetratin, were split into two parts and each fragment was terminated with a cysteine...
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Development of a System for Biosynthesis, Isolation and Purification of the Holoform of Recombinant Human Neuroglobin and Its Characteristics
Abstract—An efficient system for the biosynthesis, isolation and purification of recombinant human neuroglobin has been developed and optimized,...
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Engineering of a thermo-alkali-stable lipase from Rhizopus chinensis by rational design of a buried disulfide bond and combinatorial mutagenesis
To improve the thermostability of the lipase (r27RCL) from Rhizopus chinensis through rational design, a newly introduced buried disulfide bond...
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Integrated single-molecule real-time sequencing and RNA sequencing reveal the molecular mechanisms of salt tolerance in a novel synthesized polyploid genetic bridge between maize and its wild relatives
BackgroundTripsacum dactyloides (2 n = 4 x = 72) and Zea perennis (2 n = 4x = 40) are tertiary gene pools of Zea mays L. and exhibit many abiotic...
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Molecular Dynamic Study on the Structure and Thermal Stability of Mutant Pediocin PA-1 Peptides Engineered with Cysteine Substitutions
Pediocin and analogous bacteriocins, valued for thermal stability, serve as versatile antimicrobials in the food sector. Improving their resilience...
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Role of the interaction between troponin T and AMP deaminase by zinc bridge in modulating muscle contraction and ammonia production
The N-terminal region of troponin T (TnT) does not bind any protein of the contractile machinery and the role of its hypervariability remains...
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B-Cell Epitope Prediction for Antipeptide Paratopes with the HAPTIC2/HEPTAD User Toolkit (HUT)
B-cell epitope prediction is key to develo** peptide-based vaccines and immunodiagnostics along with antibodies for prophylactic, therapeutic...
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Characterization of oxidative stress-induced cgahp, a gene coding for alkyl hydroperoxide reductase, from industrial importance Corynebacterium glutamicum
Alkyl hydroperoxide reductase (Ahp), comprised of four different subunits AhpC, AhpD, AhpE, and AhpF, is a thiol-based antioxidative enzyme with the...
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Thiol Redox Proteomics for Identifying Redox-Sensitive Cysteine Residues Within the Protein of Interest During Stress
Redox modulation is a common posttranslational modification to regulate protein activity. The targets of oxidizing agents are cysteine...