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DNA double-strand break–capturing nuclear envelope tubules drive DNA repair
Current models suggest that DNA double-strand breaks (DSBs) can move to the nuclear periphery for repair. It is unclear to what extent human DSBs...
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Di- and tri-methylation of histone H3K36 play distinct roles in DNA double-strand break repair
Histone H3 Lys36 (H3K36) methylation and its associated modifiers are crucial for DNA double-strand break (DSB) repair, but the mechanism governing...
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Fatty acid oxidation facilitates DNA double-strand break repair by promoting PARP1 acetylation
DNA repair is a tightly coordinated stress response to DNA damage, which is critical for preserving genome integrity. Accruing evidence suggests that...
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Germline specific genes increase DNA double-strand break repair and radioresistance in lung adenocarcinoma cells
In principle, germline cells possess the capability to transmit a nearly unaltered set of genetic material to infinite future generations, whereas...
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DNA double-strand break-free CRISPR interference delays Huntington’s disease progression in mice
Huntington’s disease (HD) is caused by a CAG repeat expansion in the huntingtin ( HTT ) gene. CRISPR-Cas9 nuclease causes double-strand breaks (DSBs)...
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Boric Acid Affects the Expression of DNA Double-Strand Break Repair Factors in A549 Cells and A549 Cancer Stem Cells: An In Vitro Study
DNA double-strand break (DSB) repair genes interact with tumor stemness- and resistance-associated processes in cancer stem cells (CSCs). Therefore,...
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The type IIS restriction enzyme MmeI can cut across a double-strand break
BackgroundType-IIS restriction enzymes cut outside their recognition sites, allowing them to remove their binding sites upon digestion. This feature...
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Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag
Cas9 can cleave DNA in both blunt and staggered configurations, resulting in distinct editing outcomes, but what dictates the type of Cas9 incisions...
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Prolonged Particulate Hexavalent Chromium Exposure Induces DNA Double-Strand Breaks and Inhibits Homologous Recombination Repair in Primary Rodent Lung Cells
Hexavalent chromium [Cr(VI)] is a known lung carcinogen and a driving mechanism in human lung cells for Cr(VI)-induced lung cancer is chromosome...
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Fanconi anemia associated protein 20 (FAAP20) plays an essential role in homology-directed repair of DNA double-strand breaks
FAAP20 is a Fanconi anemia (FA) protein that associates with the FA core complex to promote FANCD2/FANCI monoubiquitination and activate the damage...
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Failure of DNA double-strand break repair by tau mediates Alzheimer’s disease pathology in vitro
DNA double-strand break (DSB) is the most severe form of DNA damage and accumulates with age, in which cytoskeletal proteins are polymerized to...
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Liquid-liquid phase separation in DNA double-strand breaks repair
DNA double-strand breaks (DSBs) are the fatal type of DNA damage mostly induced by exposure genome to ionizing radiation or genotoxic chemicals. DSBs...
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Inhibition of key DNA double strand break repair protein kinases enhances radiosensitivity of head and neck cancer cells to X-ray and proton irradiation
Ionising radiation (IR) is widely used in cancer treatment, including for head and neck squamous cell carcinoma (HNSCC), where it induces significant...
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Meiotic pairing and double-strand break formation along the heteromorphic threespine stickleback sex chromosomes
Double-strand break repair during meiosis is normally achieved using the homologous chromosome as a repair template. Heteromorphic sex chromosomes...
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Time-resolved microfluidics unravels individual cellular fates during double-strand break repair
BackgroundDouble-strand break repair (DSBR) is a highly regulated process involving dozens of proteins acting in a defined order to repair a DNA...
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Assessing the Effect of Histone Deacetylase Inhibitors on DNA Double-Strand Break Repair by Nonhomologous End Joining
This book chapter describes a plasmid-based reporter method, first described by Bennardo et al. (2008) that we use in our laboratory for determining...
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A computational approach to quantifying miscounting of radiation-induced double-strand break immunofluorescent foci
Immunofluorescent tagging of DNA double-strand break (DSB) markers, such as γ -H2AX and other DSB repair proteins, are powerful tools in understanding...
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Target residence of Cas9-sgRNA influences DNA double-strand break repair pathway choices in CRISPR/Cas9 genome editing
BackgroundDue to post-cleavage residence of the Cas9-sgRNA complex at its target, Cas9-induced DNA double-strand breaks (DSBs) have to be exposed to...
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Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis
Mammalian spermatogenesis is a heat-vulnerable process that occurs at low temperatures, and elevated testicular temperatures cause male infertility....
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