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Fluorescent Proteins
This chapter is dedicated to Fluorescent Proteins, divided according to their spectral characteristics into Blue, Cyan, Yellow, Green, Orange, Red,...
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mScarlet3: a brilliant and fast-maturing red fluorescent protein
We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record...
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Bright and stable monomeric green fluorescent protein derived from StayGold
The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell...
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Adaptable, turn-on maturation (ATOM) fluorescent biosensors for multiplexed detection in cells
A grand challenge in biosensor design is to develop a single-molecule, fluorescent protein-based platform that can be easily adapted to recognize...
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Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporters
Naturally occurring fluorescent proteins (FPs) are the most widely used tools for tracking cellular proteins and sensing cellular events. Here, we...
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Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein–Protein Interactions in Living Cells
Bimolecular fluorescence complementation (BiFC) assay is a method to visualize the protein–protein interaction in living cells. This technique is...
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Fluorescent Labeling of Outer Membrane Proteins Using the SpyCatcher-SpyTag System
The SpyCatcher-SpyTag system has become a popular and versatile tool for protein ligation. It is based on a small globular protein (SpyCatcher) that...
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A highly photostable and bright green fluorescent protein
The low photostability of fluorescent proteins is a limiting factor in many applications of fluorescence microscopy. Here we present StayGold, a...
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Assay of Reactive Oxygen/Nitrogen Species (ROS/RNS) in Arabidopsis Peroxisomes Through Fluorescent Protein Containing a Type 1 Peroxisomal Targeting Signal (PTS1)
Plant peroxisomes have an active nitro-oxidative metabolism. However, the assay of reactive oxygen and nitrogen species (ROS/RNS) could be a...
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Low protein expression enhances phenotypic evolvability by intensifying selection on folding stability
Protein abundance affects the evolution of protein genotypes, but we do not know how it affects the evolution of protein phenotypes. Here we...
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Quantitative assessment of near-infrared fluorescent proteins
Recent progress in fluorescent protein development has generated a large diversity of near-infrared fluorescent proteins (NIR FPs), which are rapidly...
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Using ER-Targeted Photoconvertible Fluorescent Proteins in Living Plant Cells
Photoconvertible fluorescent proteins (pcFPs) enable differential coloring of a single organelle. Several pcFP-based probes have been targeted to the...
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Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells
Fluorescent RNAs, aptamers that bind and activate small fluorogenic dyes, have provided a particularly attractive approach to visualizing RNAs in...
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FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs
With the development of fluorescent proteins (FPs) and advanced optical microscopy techniques, Förster or fluorescence resonance energy transfer...
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Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application
To perform correlation analysis between different physiological parameters using fluorescent protein-based functional probes, diversification of...
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Chemically stable fluorescent proteins for advanced microscopy
We report the rational engineering of a remarkably stable yellow fluorescent protein (YFP), ‘hyperfolder YFP’ (hfYFP), that withstands chaotropic...
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Forster Resonance Energy Transfer (FRET) to Visualize Protein–Protein Interactions in the Plant Cell
Forster resonance energy transfer (FRET) is an efficient method to visualize the protein–protein interaction in living cells. This technique is based...
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Observing and Quantifying Fluorescent Reporters
Genetically encoded fluorescent reporters take advantage of C. elegans’ transparency to allow non-invasive, in vivo observation, and recording of...
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Fluorescent Translocation Reporters for Sub–plasma Membrane cAMP Imaging
A wide range of fluorescent sensors with different properties have been developed for imaging of cAMP signals in living cells and tissues. Most cAMP...