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Cryo-Electron Microscopy of Enveloped Viruses Using an Upgraded Transmission Electron Microscope: Influenza Type A and B Viruses and SARS-CoV-2
AbstractCryo-electron microscopy (cryo-EM) is indispensable for the structural studies of enveloped viruses: dangerous pathogens of humans and...
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Fundamentals of Instrumentation and Electron Optics for Cryo-Electron Tomography
This chapter takes a close look at the hardware side of transmission electron microscopy and, by extension, its applications in cryo-electron...
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Determining Macromolecular Structures Using Cryo-Electron Microscopy
Structural insights into macromolecular and protein complexes provide key clues about the molecular basis of the function. Cryogenic electron...
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An electron counting algorithm improves imaging of proteins with low-acceleration-voltage cryo-electron microscope
Relative to the 300-kV accelerating field, electrons accelerated under lower voltages are potentially scattered more strongly. Lowering the...
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Beam-Induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography
Beam induced motion (BIM) is a major cause of information loss in single-particle cryo-electron microscopy (cryo-EM) and cryo-electron tomography...
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Cryo-Electron Tomography of Reconstituted Biomolecular Condensates
The assembly of membraneless compartments by phase separation has recently been recognized as a mechanism for spatial and temporal organization of...
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Cryo-Electron Microscopy at the Biological Faculty of Moscow State University
AbstractThe paper reports an example of a successful upgrade of the JEOL JEM-2100 analytical transmission electron microscope to a low-resolution...
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In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques
In situ cryo-electron tomography is undergoing a revolution as more challenging samples are moving into the center of attention. The complexity of...
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Cryogenic Preparations of Biological Specimens for Cryo-Electron Tomography
Cryo-electron tomography (cryo-ET) has become a method of choice for in-cell structural biology studies by integrating the non-invasive power of...
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Electron Tomography of Cryo-Fixed and Resin-Embedded Samples
Room-temperature electron tomography of sections prepared from high-pressure frozen, freeze-substituted and resin-embedded cells is a powerful method...
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Structural Analysis of Protein Complexes by Cryo-Electron Microscopy
Structural studies of bio-complexes using single particle cryo-Electron Microscopy (cryo-EMCryo- electron microscopy (cryo-EM)) is nowadays a...
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Structural Determination of Glucosyltransferase C by Cryo-Electron Microscopy
Biofilm formation is a critical factor in the development of cariogenic virulence of Streptococcus mutans. S. mutans has evolved a concerted...
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HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography
Cryo-focused ion beam (cryo-FIB) milling technology has been developed for the fabrication of cryo-lamella of frozen native specimens for study by in...
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Automated Cryo Electron Tomography Data Collection
Electron tomography entails the three-dimensional reconstruction of an object from its projections, which are acquired using a transmission electron...
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In Situ Structure Determination of Bacterial Surface Nanomachines Using Cryo-Electron Tomography
Bacterial surface nanomachines are often refractory to structural determination in their intact form due to their extensive association with the cell...
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Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology
Cryogenic electron microscopy and data processing enable the determination of structures of isolated macromolecules to near-atomic resolution....
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Metallo-supramolecular branched polymer protects particles from air-water interface in single-particle cryo-electron microscopy
Recent technological breakthroughs in single-particle cryo-electron microscopy (cryo-EM) enable rapid atomic structure determination of biological...
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Parallel cryo electron tomography on in situ lamellae
In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in recent years as a powerful technique for structural studies...
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Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP
Cryo-correlative light and electron microscopy (cryoCLEM) is a powerful strategy to high resolution imaging in the unperturbed hydrated state. In...
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MeasureIce: accessible on-the-fly measurement of ice thickness in cryo-electron microscopy
Ice thickness is arguably one of the most important factors limiting the resolution of protein structures determined by cryo-electron microscopy...
