Search
Search Results
-
CombFold: predicting structures of large protein assemblies using a combinatorial assembly algorithm and AlphaFold2
Deep learning models, such as AlphaFold2 and RosettaFold, enable high-accuracy protein structure prediction. However, large protein complexes are...
-
CRISPR/Cas9-based toolkit for rapid marker recycling and combinatorial libraries in Komagataella phaffii
AbstractKomagataella phaffii , a nonconventional yeast, is increasingly attractive to researchers owing to its posttranslational modification...
-
Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations
Multiplexed genetic perturbations are critical for testing functional interactions among coding or non-coding genetic elements. Compared to...
-
scifi-ATAC-seq: massive-scale single-cell chromatin accessibility sequencing using combinatorial fluidic indexing
Single-cell ATAC-seq has emerged as a powerful approach for revealing candidate cis-regulatory elements genome-wide at cell-type resolution. However,...
-
Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq
Pooled CRISPR screens coupled with single-cell RNA-sequencing have enabled systematic interrogation of gene function and regulatory networks. Here,...
-
Combinatorial selective ER-phagy remodels the ER during neurogenesis
The endoplasmic reticulum (ER) employs a diverse proteome landscape to orchestrate many cellular functions, ranging from protein and lipid synthesis...
-
Single-cell map** of combinatorial target antigens for CAR switches using logic gates
Identification of optimal target antigens that distinguish cancer cells from normal surrounding tissue cells remains a key challenge in chimeric...
-
A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells
Alterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular...
-
Identification of clinically relevant T cell receptors for personalized T cell therapy using combinatorial algorithms
A central challenge in develo** personalized cancer cell immunotherapy is the identification of tumor-reactive T cell receptors (TCRs). By...
-
Combinatorial design of nanoparticles for pulmonary mRNA delivery and genome editing
The expanding applications of nonviral genomic medicines in the lung remain restricted by delivery challenges. Here, leveraging a high-throughput...
-
Combinatorial fluorescent labeling of live anaerobic bacteria via the incorporation of azide-modified sugars into newly synthesized macromolecules
The human gut microbiome modulates physiological functions and pathologies; however, a mechanistic understanding of microbe–host and microbe–microbe...
-
Combinatorial Effect of Mesenchymal Stem Cells and Extracellular Vesicles in a Hydrogel on Cartilage Regeneration
Background:Mesenchymal stem cells (MSCs) are used for tissue regeneration due to their wide differentiation capacity and anti-inflammatory effects....
-
Combinatorial single-cell profiling of major chromatin types with MAbID
Gene expression programs result from the collective activity of numerous regulatory factors. Studying their cooperative mode of action is imperative...
-
FIPRESCI: droplet microfluidics based combinatorial indexing for massive-scale 5′-end single-cell RNA sequencing
Single-cell RNA sequencing methods focusing on the 5′-end of transcripts can reveal promoter and enhancer activity and efficiently profile immune...
-
Omics and imaging combinatorial approach reveals butyrate-induced inflammatory effects in the zebrafish gut
BackgroundPrebiotic feed additives aim to improve gut health by influencing the microbiota and the gut barrier. Most studies on feed additives...
-
Progress in single-cell multimodal sequencing and multi-omics data integration
With the rapid advance of single-cell sequencing technology, cell heterogeneity in various biological processes was dissected at different omics...
-
Metabolomics and transcriptomics based multi-omics integration reveals radiation-induced altered pathway networking and underlying mechanism
Recent advancement in integrated multi-omics has significantly contributed to many areas of the biomedical field. Radiation research has also grasped...
-
Mosaic integration and knowledge transfer of single-cell multimodal data with MIDAS
Integrating single-cell datasets produced by multiple omics technologies is essential for defining cellular heterogeneity. Mosaic integration, in...
-
Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases
Conventional genome engineering with CRISPR–Cas9 creates double-strand breaks (DSBs) that lead to undesirable byproducts and reduce product purity....
-
Pheniqs 2.0: accurate, high-performance Bayesian decoding and confidence estimation for combinatorial barcode indexing
BackgroundSystems biology increasingly relies on deep sequencing with combinatorial index tags to associate biological sequences with their sample,...
