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Development of a CRISPR-Cas12a based assay for the detection of swine enteric coronaviruses in pig herds in China
Porcine epidemic diarrhea virus (PEDV), Transmissible gastroenteritis virus (TGEV), Porcine deltacoronavirus (PDCoV) and Swine acute diarrhea...
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Establishing a pulmonary aspergillus fumigatus infection diagnostic platform based on RPA-CRISPR-Cas12a
In this study, we devised a diagnostic platform harnessing a combination of recombinase polymerase amplification (RPA) and the clustered regularly...
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Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations
Multiplexed genetic perturbations are critical for testing functional interactions among coding or non-coding genetic elements. Compared to...
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Prime editing using CRISPR-Cas12a and circular RNAs in human cells
Genome editing with prime editors based on CRISPR-Cas9 is limited by the large size of the system and the requirement for a G/C-rich...
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Profiling the impact of the promoters on CRISPR-Cas12a system in human cells
The plasmid vector platform is the most commonly used vector for the expression of the versatile CRISPR-Cas technique and the promoter is a crucial...
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Engineered circular guide RNAs boost CRISPR/Cas12a- and CRISPR/Cas13d-based DNA and RNA editing
BackgroundThe CRISPR/Cas12a and CRISPR/Cas13d systems are widely used for fundamental research and hold great potential for future clinical...
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An efficient CRISPR–Cas12a promoter editing system for crop improvement
Promoter editing represents an innovative approach to introduce quantitative trait variation (QTV) in crops. However, an efficient promoter editing...
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Plant Genome Editing Mediated by CRISPR/Cas12a System
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system has emerged as an efficient genome editing tool. CRISPR/Cas12a...
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Application of DETECTR for Selective Detection of Bacterial Phytopathogen Dickeya solani Using Recombinant CRISPR-Nuclease Cas12a Obtained by Single-Stage Chromatographic Purification
AbstractThe work demonstrates that recombinant CRISPR-nuclease Cas12a, purified after heterologous expression with a simplified method using...
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Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line
Clustered regularly interspaced short palindromic repeats (CRISPR) technology is a powerful genome editing tool. Recently developed CRISPR-Cas12a...
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A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) ty** assay
Persistent infection with high-risk human papillomavirus (HR-HPV) is the primary and initiating factor for cervical cancer. With over 200 identified...
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Genetic identification of Staphylococcus aureus isolates from cultured milk samples of bovine mastitis using isothermal amplification with CRISPR/Cas12a-based molecular assay
Bovine mastitis, a common and costly disease in dairy cattle, is primarily caused by Staphylococcus aureus . Timely and accurate detection of this...
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Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a
Gonorrhea, caused by Neisseria gonorrhoeae ( N. gonorrhoeae ), is a persistent global public health threat. The development of low-cost, point-of-care...
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Development of a rapid, accurate, and field deployable LAMP-CRISPR-Cas12a integrated assay for Xylella fastidiosa detection and surveillance
Xylella fastidiosa is an aggressive plant pathogenic bacterium of significant quarantine concern. Accurate and reliable detection tools are essential...
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An enzymatic recombinase amplification assay combined with CRISPR-Cas12a for the rapid detection of acute hepatopancreatic necrosis disease in shrimp Penaeus vannamei
The shrimp farming sector has faced significant hurdles due to acute hepatopancreatic necrosis disease (AHPND). In this study, an attempt was made to...
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One-pot platform for rapid detecting virus utilizing recombinase polymerase amplification and CRISPR/Cas12a
AbstractThe livestock industry has been deeply affected by African swine fever virus (ASFV) and Capripoxvirus (CaPV), which caused an enormous...
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Non-nucleic acid extraction and ultra-sensitive detection of African swine fever virus via CRISPR/Cas12a
AbstractEarly diagnosis of the African swine fever virus (ASFV) is the main preventive measure for ASFV. Here, we developed a fluorescent biosensor...
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RPA/CRISPR-cas12a as a specific, sensitive and rapid method for diagnosing Ehrlichia canis and Anaplasma platys in dogs in Thailand
Rickettsial pathogens including Ehrlichia canis and Anaplasma platys are bacteria that cause parasitic infections in dogs such as canine monocytic...
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Reverse transcription-recombinase-aided amplification and CRISPR/Cas12a-based visual detection of maize chlorotic mottle virus
Maize chlorotic mottle virus (MCMV) is one of the important quarantine pathogens in China. It often co-infects with one or two viruses in the family Potyviridae...
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CRISPR Cas9- and Cas12a-mediated gusA editing in transgenic blueberry
To develop an effective genome editing tool for blueberry breeding, CRISPR-Cas9 and CRISPR-Cas12a were evaluated for their editing efficiencies of a...
