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Isolation of Human Adipose-Derived Stem Cells
Human adipose-derived stromal/stem cells (hASCs) are a promising source of adult stem cells used in numerous applications in regenerative medicine....
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Combination of Adipose-Derived Stromal/Stem Cells and Decellularized Adipose Tissue Hydrogel for Osteogenic Applications
Adipose-derived stromal/stem cells (ASCs) and decellularized adipose tissue (DAT) are adipose tissue products obtained from individuals undergoing...
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Isolation of Human Adipose-Derived Stromal/Stem Cells from Lipoaspirates
Adipose tissue is an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative...
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Generating and Characterizing Adipose Spheroids from Adipose-Derived Stromal/Stem Cells
Advances in technology and automation over the past several decades have made it feasible to perform high-throughput compound screening with cell...
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Standard Operating Procedure for Production of Mouse Brown Adipose Tissue-Derived Mesenchymal Stem Cells
Over the past years, stem cell technology was heralded as a significant breakthrough of the century in scrutinizing the intricacies of human body...
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A Sequential Culturing System for Generating Epithelial-Like Stem Cells from Human Mesenchymal Stem Cells Derived from Adipose Tissue
AbstractSkin burn repair requires efficient regeneration in order to achieve proper healing. We applied a sequential culturing system 2D1w/3D2w...
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Osteopontin Rejuvenates Senescent Adipose-Derived Stem Cells and Restores their Bone Tissue Regenerative Function
The regenerative function of stem cells is compromised when the proportion of senescent stem cells increases with ageing advance. Therefore,...
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Immunophenotype of Mesenchymal Stem Cells Derived from Epicardial and Perivascular Adipose Tissue in Patients with Cardiovascular Diseases
AbstractThe work was aimed to evaluate the immunophenotype of adipose tissue stem cells isolated from epicardial and perivascular fat depots in...
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Isolation of Murine Adipose-Derived Stromal/Stem Cells Using an Explant Culture Method
Adipose tissue provides a valuable cell source for tissue engineering, regenerative medicine, and adipose tissue biology studies. The most widely...
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Obesity-driven mitochondrial dysfunction in human adipose tissue-derived mesenchymal stem/stromal cells involves epigenetic changes
Obesity exacerbates tissue degeneration and compromises the integrity and reparative potential of mesenchymal stem/stromal cells (MSCs), but the...
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Spheroid Formation of Human Adipose-Derived Stem Cells Using a Liquid Overlay Technique
Compared to two-dimensional monolayer culture, cells cultured in three-dimensional (3D) platforms provide a more biochemically and physiologically...
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Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic and Osteogenic Differentiation or Flow Cytometry-Based Analysis
Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders....
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Adipose-Derived Mesenchymal Stem Cells and Their Derived Epidermal Progenitor Cells Conditioned Media Ameliorate Skin Aging in Rats
BACKGROUND: Skin alterations are among the most prominent signs of aging, and they arise from both intrinsic and extrinsic factors that interact and...
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Schizophyllan promotes osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro
BackgroundBioactive polysaccharides are a promising way for bone disease prevention with high efficiency. Schizophyllan (SPG) is a polysaccharide...
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Canine Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation, Characterization, and Differentiation
Adult mesenchymal stromal/stem cells (MSCs) are a standard component of de novo tissue generation to treat and study injury, disease, and...
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The Lower in Vivo Osteogenicity of Adipose Tissue-Derived Stem Cells Correlates with a Higher Innate Immune Response
Adipose tissue-derived mesenchymal stem cells (ATSCs) have been used as an alternative to bone marrow-derived mesenchymal stem cells (BMSCs) for bone...
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Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation
Cats are among the most popular household pets. However, compared to other species, there is little information specific to feline adult mesenchymal...
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Oxaloacetate as new inducer for osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro
BackgroundMitochondrial organelles play a crucial role in cellular metabolism so different cell types exhibit diverse metabolic and energy demands....
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Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: not as effective as cells
BackgroundAlthough the paracrine effects of mesenchymal stem/stromal cells (MSCs) have been recognized as crucial mediators of their regenerative...
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Superiority of Adipose-derived CD34 + Cells over Adipose-derived Stem Cells in Promoting Ischemic Tissue Survival
BackgroundTissue ischemia usually leads to necrosis and is a threatening condition associated with reconstructive surgery. Promoting the survival of...
