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Measurement of Mucinase Activity
Mucinase consists of some proteases, glycosidases, sulfatases, and sialidases. It is not practical to measure individual enzyme activities when...
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Recording Cilia Activity in Ctenophores
Pelagic ctenophores swim in the water with the help of eight rows of long fused cilia. Their entire behavioral repertoire is dependent to a large...
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Measure of Peptidoglycan Degradation Activity
Most bacterial secretion systems are large machines that cross the cell envelopeCell envelopes to deliver effectors outside the cell or directly into...
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Activity Patterns of American Marsupials
New World marsupials have a striking diversity of activity patterns. Until now, knowledge on diel activity was normally granted by means of simple...
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Physical Activity Across the Life Span: Personality, Physical Activity, and Sedentary Behavior
In this chapter, the prevalence of physically activity as well as sedentary behavior across the life span is presented. Empirical findings on the...
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Spectrophotometric Assays for Measuring Hydroxypyruvate Reductase Activity
Hydroxypyruvate reductase (HPR; EC 1.1.1.81) activity is integral to the photorespiratory pathway. Within photorespiration, HPR catalyzes the...
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Evaluation of Connexin Hemichannel Activity In Vivo
Connexin hemichannels (Cx HCs) are hexameric structures at the cell plasma membrane, whose function as membrane transport proteins allows for the...
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Radiometric Assay of ABHD2 Activity
The α,β-hydrolase fold-containing protein 2 (ABHD2) is a serine hydrolase, responsible for the cleavage of endogenous 2-arachidonoylglycerol (2-AG)....
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Assay of Hemolytic Activity
Hemolysis refers to the breakdown of erythrocytes/red blood cells (RBCs). This results in the destruction of erythrocytes, releasing hemoglobin from...
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Antimicrobial Activity of Capsaicin and Its Derivatives
Chilies contain a special group of alkaloids called capsaicinoids, which are synthesized as secondary metabolites and are responsible for the...
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Enzyme Identification and Screening: Activity-Based Methods
Methods for the identification and characterization of “ideal” enzymes for industrial use in the field of biotechnology have developed rapidly in...
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Assay of DAGLα/β Activity
The endocannabinoid 2-arachidonoylglycerol (2-AG) exerts its physiological action by binding to and functionally activating type-1 (CB1) and type-2...
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Assay of NAT Activity
In animal tissues, N-acyltransferase (NAT) catalyzes the first reaction in the biosynthetic pathway of bioactive...
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Assay of Monoacylglycerol Lipase Activity
Monoacylglycerol lipase (MGL/MAGL/MGLL) is a serine hydrolase involved in the biological deactivation of the endocannabinoid...
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Hemolytic Activity
Hemolysis is the most commonly used method to evaluate toxicity for therapies administered intravenously. Red blood cells (RBCs) are separated using...
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Radiometric Assay of FAAH Activity
Fatty acid amide hydrolase (FAAH) is an intracellular enzyme responsible for the hydrolysis of endogenous anandamide (AEA), a reaction that...
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Quantitative Determination of Antibacterial Activity During Bacterial Coculture
Antibacterial activity assays are an important tool in the assessment of the ability of one bacterium to kill or inhibit the growth of another, for...
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Fluorimetric Assay of FAAH Activity
Fatty acid amide hydrolase (FAAH) is the enzyme responsible for the degradation of anandamide (N-arachidonoylethanolamine, AEA) to arachidonic acid...
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Assessing the Activity of Transcription Factor FoxO1
The transcription factor FoxO1 (forkhead box O1) regulates genes that are involved in development, metabolism, cellular innovation, longevity, and...
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Fluorescence-Based NAPE-PLD Activity Assay
N-Acylphosphatidylethanolamine phospholipase D (NAPE-PLD) is regarded as the principal enzyme that generates N-acylethanolamines (NAEs), a family of...