Search
Search Results
-
Biological role of the major AP (abasic site) endonuclease of an archaeon from geothermal environments
Archaea and bacteria in geothermal environments are predicted to suffer DNA depurination in vivo at high rates, which raises questions regarding the...
-
Biochemical Assessment of the Mutant Sliding β-Clamp on Stimulation of Endonuclease IV from Staphylococcus aureus
Staphylococcus aureus is a pathogenic bacterium that causes various infections in humans. The emergence of methicillin-resistant Staphylococcus aureus ...
-
The activity of yeast Apn2 AP endonuclease at uracil-derived AP sites is dependent on the major carbon source
Yeast Apn2 is an AP endonuclease and DNA 3′-diesterase that belongs to the Exo III family with homology to the E. coli exonuclease III, Schizosaccharom...
-
Effect of DNA Methylation on the 3′→5′ Exonuclease Activity of Major Human Abasic Site Endonuclease APEX1
AbstractApurinic/apyrimidinic (AP) endonucleases are the key enzymes in the DNA base excision repair, as they hydrolyze the phosphodiester bond in...
-
A unique DNA-binding mode of African swine fever virus AP endonuclease
African swine fever virus (ASFV) is highly contagious and can cause lethal disease in pigs. ASFV is primarily replicated in the cytoplasm of pig...
-
Biochemical and functional characterization of an endonuclease III from Thermococcus barophilus Ch5
Endonuclease III (EndoIII) is a bifunctional DNA glycosylase that is essential to excise thymine glycol (Tg) from DNA. Although EndoIII is widespread...
-
Characterization of Recombinant Endonuclease IV from Mycobacterium tuberculosis
AbstractMycobacterium tuberculosis cells contain two apurinic/apyrimidinic (AP) endonucleases, endonuclease IV (MtbEnd) and exonuclease III...
-
5'-Deoxyribose Phosphate Lyase Activity of Apurinic/Apyrimidinic Endonuclease 1
AbstractOne of the most common DNA lesions is the appearance of apurinic/apyrimidinic (AP-) sites. The main repair pathway for AP sites is initiated...
-
The APE1/REF-1 and the hallmarks of cancer
APE1/REF-1 (apurinic/apyrimidinic endonuclease 1 / redox factor-1) is a protein with two domains, with endonuclease function and redox activity. Its...
-
DNA Probes for Analysis of the Activity of Key Enzymes of the Base Excision DNA Repair Pathway in Human Cells
Abstract —The important role of DNA damage in the occurrence of various diseases, including cancer, has led to study of the mechanisms of genetic...
-
AP endonuclease 1 (Apex1) influences brain development linking oxidative stress and DNA repair
Brain and neurons are particularly sensitive to reactive oxygen species (ROS). Oxidative damage from ROS results in increased 8-oxoguanine in DNA...
-
Mutational and Kinetic Analysis of APE1 Endoribonuclease Activity
AbstractHuman apurinic/apyrimidinic endonuclease 1 (APE1) participates in the DNA repair system. It is believed that the main biological function of...
-
Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice Using Endogenous Streptavidin/FLAG Tags
Biological complexity is achieved through elaborate interactions between relatively few individual components. Affinity purification (AP) has allowed...
-
Common occurrence of hotspots of single strand DNA breaks at transcriptional start sites
BackgroundWe recently developed two high-resolution methods for genome-wide map** of two prominent types of DNA damage, single-strand DNA breaks...
-
Influence of the Poly(ADP-Ribose) Polymerase 1 Level on the Status of Base Excision Repair in Human Cells
AbstractBase excision repair (BER) is aimed at repair of damaged bases, which are the largest group of DNA lesions. The main steps of BER are...
-
Gene editing with ‘pencil’ rather than ‘scissors’ in human pluripotent stem cells
Owing to the advances in genome editing technologies, research on human pluripotent stem cells (hPSCs) have recently undergone breakthroughs that...
-
Identification, cloning and characterization of AcMSH1 from Onion (Allium cepa L.)
BackgroundMSH1 (MutS homolog1) is a nuclear-encoded protein that plays a crucial role in maintaining low mutation rates and stability of the...
-
Small-molecule inhibition of APE1 induces apoptosis, pyroptosis, and necroptosis in non-small cell lung cancer
Apurinic/apyrimidinic endonuclease 1 (APE1) plays a critical role in the base excision repair (BER) pathway, which is responsible for the excision of...
-
APEX1 Nuclease and Redox Functions are Both Essential for Adult Mouse Hematopoietic Stem and Progenitor Cells
Self-renewal and differentiation of hematopoietic stem and progenitor cells (HSPCs) are carefully controlled by extrinsic and intrinsic factors, to...
-
Pirh2 modulates the mitochondrial function and cytochrome c-mediated neuronal death during Alzheimer’s disease
Pirh2 is an E3 ubiquitin ligase known to regulate the DNA damage responses through ubiquitylation of various participating signaling factors. DNA...
