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The effect of the 13C abundance of soil microbial DNA on identifying labelled fractions after ultracentrifugation
AbstractDNA-based stable isotope probing (DNA-SIP) technology has been widely employed to trace microbes assimilating target substrates. However, the...
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[13C]bicarbonate labelled from hyperpolarized [1-13C]pyruvate is an in vivo marker of hepatic gluconeogenesis in fasted state
Hyperpolarized [1- 13 C]pyruvate enables direct in vivo assessment of real-time liver enzymatic activities by 13 C magnetic resonance. However, the...
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Preparation of uniformly labelled 13C- and 15N-plants using customised growth chambers
BackgroundStable isotopically labelled organisms have found wide application in life science research including plant physiology, plant stress and...
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A model for determining cardiac mitochondrial substrate utilisation using stable 13C-labelled metabolites
IntroductionRelative oxidation of different metabolic substrates in the heart varies both physiologically and pathologically, in order to meet...
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Colloidal gold and fluorescent immunochromatographic test strips for canine parvovirus detection
AbstractCanine parvovirus (CPV) is an acute and highly infectious virus causing disease in puppies and, thus, affecting the global dog industry. The...
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13C labelling reveals details of the soybean (Glycine max (L.) Merrill) seedling metabolic network
Seedlings depend upon seed reserves for the prevision of carbon skeletons for growth and energy production. Post-germinative growth of soybean is...
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A Mechanism to Transform Complex Salicinoids with Caffeoylquinic Acids in Lepidopteran Specialist Herbivores (Notodontidae)
Larvae of the Salicaceae-adapted Notodontidae have developed a unique mechanism to metabolize the chemical defenses of their Salicaceae host plants....
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Phytate metabolism is mediated by microbial cross-feeding in the gut microbiota
Dietary intake of phytate has various reported health benefits. Previous work showed that the gut microbiota can convert phytate to short-chain fatty...
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Algal methylated compounds shorten the lag phase of Phaeobacter inhibens bacteria
The lag phase is key in resuming bacterial growth, but it remains underexplored particularly in environmental bacteria. Here we use transcriptomics...
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Cryo-electron tomography of intact cardiac muscle reveals myosin binding protein-C linking myosin and actin filaments
Myosin binding protein C (MyBP-C) is an accessory protein of the thick filament in vertebrate cardiac muscle arranged over 9 stripes of intervals of...
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Decomposition of Apple (Malus domestica) Plant Residue and Uptake of Residue-Derived N
This study examined the decomposition dynamics of apple ( Malus domestica ) plant residue (senesced leaves and pruned branches) and the uptake of...
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Development and application of a colloidal-gold immunochromatographic strip for detecting Getah virus antibodies
AbstractGetah virus (GETV) is a re-emerging mosquito-borne alphavirus that is highly pathogenic, mainly to pigs and horses. There are no vaccines or...
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Trigonelline is an NAD+ precursor that improves muscle function during ageing and is reduced in human sarcopenia
Mitochondrial dysfunction and low nicotinamide adenine dinucleotide (NAD + ) levels are hallmarks of skeletal muscle ageing and sarcopenia
1 –3 , but it...
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Methanol utilizers of the rhizosphere and phyllosphere of a common grass and forb host species
BackgroundManaged grasslands are global sources of atmospheric methanol, which is one of the most abundant volatile organic compounds in the...
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Oncogenic Ras expression increases cellular formate production
One-carbon units, critical intermediates for cell growth, may be produced by a variety of means, one of which is via the production of formate....
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An inter-order comparison of copepod fatty acid composition and biosynthesis in response to a long-chain PUFA deficient diet along a temperature gradient
Copepods serve as a major link in marine food webs, bridging the energy transfer from primary producers to higher trophic levels. Oceanic warming is...
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Deforestation for agriculture increases microbial carbon use efficiency in subarctic soils
Agriculture is likely to expand poleward with climate change, encouraging deforestation for agriculture in subarctic regions, which alters soil...
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A putatively new family of alphaproteobacterial chloromethane degraders from a deciduous forest soil revealed by stable isotope probing and metagenomics
BackgroundChloromethane (CH 3 Cl) is the most abundant halogenated organic compound in the atmosphere and substantially responsible for the...
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Microbial carbon use efficiency of litter with distinct C/N ratios in soil at different temperatures, including microbial necromass as growth component
An incubation study was carried out to investigate the effects of litter quality, i.e. 15 N-labelled maize (C/N of 25.5) and Rhodes grass (C/N of...
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Soil denitrification response to increased urea concentration constrains nitrous oxide emissions in a simulated cattle urine patch
AimIncorporating non-bloat legumes into grass pastures can reduce enteric methane and alter cattle urinary urea N output by increasing protein...
