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Phage DNA Extraction, Genome Assembly, and Genome Closure
Bacteriophages, or more simply phages, are currently experiencing a renaissance in life science research for their roles in natural microbial...
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Genome Rearrangement Analysis
Genome rearrangements are mutations that change the gene content of a genome or the arrangement of the genes on a genome. Several years of research...
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Comparative Genome Annotation
Newly sequenced genomes are being added to the tree of life at an unprecedented fast pace. A large proportion of such new genomes are...
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Caveats for Watermelon Whole Genome Sequencing
Watermelon is a highly important vegetable crop species worldwide, with its abundance of genetic resources aiding in identifying mechanisms and...
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Crucifer’s Pathogens Genome
The information on genome sequences, analysis and assembly of limited pathogens of crucifers has been generated and made available. It has given...
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Genome evolution: Lessons from Genolevures
In the past years, yeast genome-sequencing programs have been widely developed. Two of them, namely Genolevures I and II, were devoted to the...
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Genome Editing in Forest Trees
Since the first CRISPR/Cas-mediated genome editing of poplar in 2015, an increasing number of tree species are being genome-edited. Although the...
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Phased Genome Assemblies
The ultimate goal of de novo assembly of reads sequenced from a diploid individual is the separate reconstruction of the sequences corresponding to...
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Step-by-Step Bacterial Genome Comparison
Thanks to advancements in genome sequencing and bioinformatics, thousands of bacterial genome sequences are available in public databases. This...
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Genome Editing in Biotech Regulations Worldwide
Since the introduction of genome editing techniques in breeding and the first commercial products on the market, various governments or jurisdictions...
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Genome Editing and Transgenes in Pigs
Disruption of specific target genes is a first step to uncover the gene function. Large-sized animals are difficult to be targeted in embryo level,...
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Precision Genome Editing with CRISPR-Cas9
The CRISPR/Cas9 system is a revolutionary technology for genome editing that allows for precise and efficient modifications of DNA sequences. The...
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Genome Editing of Silkworms
Silkworm is a lepidopteran insect that has been used as a model for a wide variety of biological studies. The microinjection technique is available,...
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Prospects for Plant Genome Editing
Genome editing – the focus of this book – is a set of methods that provide opportunities to precisely and efficiently improve crop traits by...
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Genome Editing of Gene Families for Crop Improvement
Crop improvement has been a long-standing focus of agricultural research, aiming to enhance nutritional richness, aroma, visual appeal, and yield to...
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From germline genome to highly fragmented somatic genome: genome-wide DNA rearrangement during the sexual process in ciliated protists
Genomes are incredibly dynamic within diverse eukaryotes and programmed genome rearrangements (PGR) play important roles in generating genomic...
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Genome Editing of Pig
Pigs have anatomical and physiological characteristics similar to humans; therefore, genetically modified pigs have the potential to become a...
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Analyses of Nuclear Reads Obtained Using Genome Skimming
In this protocol paper, we review a set of methods developed in recent years for analyzing nuclear reads obtained from genome skimming. As the cost...
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Background: Genome Editing with Programmable Nucleases
Scientists use genome editing with programmable nucleases to carry out the targeted modification of nucleic acid. The major genome editing...
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Genome Editing in Pigs
The generation of genetically engineered (GE) pigs for disease modeling and xenotransplantation has been massively facilitated by the discovery of...