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2 in 1 Vectors Improve in Planta BiFC and FRET Analysis
Protein-protein interactions (PPIs) play vital roles in all subcellular processes, and a number of tools have been developed for their detection and...
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Purification of Recombinant N-terminal Histidine-Tagged Arabidopsis thaliana Phosphoglycolate Phosphatase 1, Glycolate Oxidase 1 and 2, and Hydroxypyruvate Reductase 1
To measure the kinetic properties of photorespiratory enzymes, it is necessary to work with purified proteins. Protocols to purify photorespiratory...
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The cell functions of phospholipase C-1, Ca2+/H+ exchanger-1, and secretory phospholipase A2 in tolerance to stress conditions and cellulose degradation in Neurospora crassa
We investigated the cell functions of the Ca 2+ signaling genes phospholipase C-1 ( plc-1 ), Ca 2+ / H + exchanger ( cpe-1 ), and secretory phospholipase A 2 ( splA...
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Effect of Salmonella pathogenicity island 1 and 2 (SPI-1 and SPI-2) deletion on intestinal colonization and systemic dissemination in chickens
Salmonella ’s virulence genes are located in two regions known as Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2). SPI-1 allows the...
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Does one plus one always equal two? Structural differences between nesfatin-1, -2, and nesfatin-1/2
Nesfatin-1 and -2 are produced from a reaction in which the N-terminus of human Nucleobindin-2 undergoes proteolytical processing. To date,...
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The CRZ-1 transcription factor binds to specific sequences in the promoters of al-2, cao-2, and ylo-1 to regulate the expression of these carotenoid biosynthesis genes in Neurospora crassa
The calcineurin-CRZ-1 signaling pathway regulates the carotenoid biosynthesis pathway in Neurospora crassa . The expression of different carotenoid...
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Cellular Assay to Study β-Arrestin Recruitment by the Cannabinoid Receptors 1 and 2
Cannabinoid receptor 1 (CB1R) and cannabinoid receptor 2 (CB2R) are G protein-coupled receptors (GPCRs) that activate a variety of pathways upon...
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Shared and distinct interactions of type 1 and type 2 Epstein-Barr Nuclear Antigen 2 with the human genome
BackgroundThere are two major genetic types of Epstein-Barr Virus (EBV): type 1 (EBV-1) and type 2 (EBV-2). EBV functions by manipulating gene...
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Small- and medium-sized rice fields identification in hilly areas using all available sentinel-1/2 images
BackgroundMastering the spatial distribution and planting area of paddy can provide a scientific basis for monitoring rice production, and planning...
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Comparative pathogenicity of SARS-CoV-2 Omicron subvariants including BA.1, BA.2, and BA.5
The unremitting emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants necessitates ongoing control measures. Given its...
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2 × 2 Tables
2 × 2 tables are used to organize data for two proportions. From 2 × 2 tables2x2 table, we compare proportions as differences or as ratios. We can...
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Characterization of the Ubiquitin and ISG15 Deconjugase Activity of SARS-CoV-1 and SARS-CoV-2 Papain-Like Protease
Both severe acute respiratory syndrome coronavirus 1 and 2 (SARS-CoV-1 and SARS-CoV-2) encode a papain-like protease (PLpro), which plays a vital...
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Structural insight into the binding mode of cefotaxime and meropenem to TEM-1, SHV-1, KPC-2, and Amp-C type beta-lactamases
Antimicrobial resistance is an emerging threat to public health around the world. The study employs computational and biophysical methods to...
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Profiling of testis-specific or testis-predominant genes expressed in mouse male germ cell lines GC-1 and GC-2
BackgroundSpermatogenesis is a tightly organized process that utilizes an intrinsic genetic program composed of germ cell-specific genes. Although...
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Nesfatin-1 and nesfatin-1-like peptide attenuate hepatocyte lipid accumulation and nucleobindin-1 disruption modulates lipid metabolic pathways
Nesfatin-1 (NESF-1) has been shown to modulate lipid metabolism. We have identified a nesfatin-1-like-peptide (NLP) processed from a related...
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DJ-1 preserves ischemic postconditioning-induced cardioprotection in STZ-induced type 1 diabetic rats: role of PTEN and DJ-1 subcellular translocation
BackgroundIschemic postconditioning (IPostC) has been reported as a promising method for protecting against myocardial ischemia-reperfusion (MI/R)...
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Characterization of two distinct immortalized endothelial cell lines, EA.hy926 and HMEC-1, for in vitro studies: exploring the impact of calcium electroporation, Ca2+ signaling and transcriptomic profiles
BackgroundDisruption of Ca 2+ homeostasis after calcium electroporation (CaEP) in tumors has been shown to elicit an enhanced antitumor effect with...
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Synthesis of Novel 2-((3-(Benzofuran-2-yl)-1-phenyl-1H-pyrazol-4-yl)methylene)hydrazinyl-4-phenylthiazole: Potent EGFR Targeting Anticancer Agents
AbstractObjective: Benzofurans, fused pyrazole systems, and thiazole moieties are the most important pharmacophores and intermediates for making...
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Exopolysaccharide (LHEPS-1) from Lactobacillus helveticus MB2-1 Induced Apoptosis and G2/M Arrest in Human Liver Cancer HepG-2 Cells
AbstractThe aim of this study was to investigate the antitumor activity of exopolysaccharide (EPS) isolated from Lactobacillus helveticus MB2-1....
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Evaluation of stability of (1R,2 S)-(−)-2-methylamino-1-phenyl-1-propanol hydrochloride in plasma and urine samples-inoculated with Escherichia coli using high-performance liquid chromatography (HPLC)
The preservation of drug stability in biological evidence during the processes of collection and storage poses a substantial obstacle to the progress...
