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Effect of Dynamic and Static Load on the Concentration of Myokines in the Blood Plasma and Content of Sodium and Potassium in Mouse Skeletal Muscles
AbstractModulation of cytokine production by physical activity is of considerable interest, since it might be a promising strategy for correcting...
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Fluorescent Techniques for RNA Detection in Nanoparticles
The utilization of drug delivery systems, such as lipid nanoparticles and polyplexes/micelleplexes, has shown promise in intracellularly delivering...
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Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry
Colla corii asini ( CCA ) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan...
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Foudroyant cerebral venous (sinus) thrombosis triggered through CLEC-2 and GPIIb/IIIa dependent platelet activation
Cerebral venous (sinus) thrombosis (CVT) is an unusual manifestation of venous thrombosis causing severe neurological impairment and seizures
1 ,2 ....
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Nattokinase-heparin exhibits beneficial efficacy and safety—an optimal strategy for CKD patients on hemodialysis
Heparin is a widely used anticoagulant in hemodialysis (HD) for patients with chronic kidney disease (CKD); however, it entails the risk of thrombus...
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COVID-19: imbalance of multiple systems during infection and importance of therapeutic choice and dosing of cardiac and anti-coagulant therapies
The renin–angiotensin–aldosterone system and its metabolites play an important role in homeostasis of body, especially the cardiovascular system. In...
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Detoxifying deoxynivalenol (DON)-contaminated feedstuff: consequences of sodium sulphite (SoS) treatment on performance and blood parameters in fattening pigs
A 10-week feeding experiment was carried out examining the effects of deoxynivalenol (DON)-contaminated maize treated with different sodium sulphite...
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α-Linolenic Acid Vesicles-Mediated Tau Internalization in Microglia
In Alzheimer’s disease, the synaptic loss is prominent due to the accumulation of Amyloid βeta (Aβ) protein in synapses, which affect...
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Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes
ObjectivesThis study aimed to investigate whether chromatography using an ExoPUA column, an affinity column for phospholipid membranes, could...
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Protective Effects of a synthetic glycosaminoglycan mimetic (OTR4132) in a rat immunotoxic lesion model of septohippocampal cholinergic degeneration
Using a partial hippocampal cholinergic denervation model, we assessed the effects of the RGTA ® named OTR4132, a synthetic heparan-mimetic biopolymer...
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Chemical O-sulfation of N-sulfoheparosan: a route to rare N-sulfo-3-O-sulfoglucosamine and 2-O-sulfoglucuronic acid
Heparosan, the capsular polysaccharide of E. coli K5 is currently used as the starting material in the chemoenzymatic synthesis of heparan sulfate...
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Metabolite Analyses Using Nuclear Magnetic Resonance (NMR) Spectroscopy in Plasma of Patients with Prostate Cancer
Nuclear magnetic resonance (NMR) spectroscopy enables the detection and the quantification of a large range of molecules, including...
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Eldecalcitol (ED-71)-induced exosomal miR-6887-5p suppresses squamous cell carcinoma cell growth by targeting heparin-binding protein 17/fibroblast growth factor–binding protein-1 (HBp17/FGFBP-1)
Heparin-binding protein 17/fibroblast growth factor–binding protein-1 (HBp17/FGFBP-1) was purified from A431 cell-conditioned media based on its...
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Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea
Whole-mount in situ hybridization (WISH), colorimetric or fluorescent (FISH), allows for the visualization of endogenous RNA. For planarians, robust...
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Purified Versus Plasma Alpha-1 Antitrypsin Effects on Cellular Activities
By adding purified exogenous alpha-1 antitrypsin (AAT) to peripheral blood mononuclear cells in 20% autologous serum and measuring AAT-induced...
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Whole-Mount In Situ Hybridization (WISH) for Salamander Embryos and Larvae
Whole-mount in situ hybridization (WISH) is widely used to visualize transcribed gene sequences (mRNA) in develo** embryos, larvae, and other...
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Evaluation of Molecular Interactions and Cellular Dynamics at the Maternal-Fetal Interface During Placental Morphogenesis
Molecular events at the maternal-fetal interface establish successful pregnancies. Identifying and characterizing the heterogeneous cell population...
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A High-Throughput Method to Profile Protein Liquid-Liquid Phase Separation
Protein liquid-liquid phase separation (LLPS) plays an essential role in the dynamic assembly of various membraneless compartments, which fulfill...
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Label-Free Identification of Persistent Particles in Association with Primary Immune Cells by Imaging Flow Cytometry
The frequency of human exposure to persistent particles via consumer products, air pollution, and work environments is a modern-day hazard and an...
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Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular Matrix
Hyaluronan (HA) is the most abundant glycosaminoglycan in the extracellular matrixExtracellular matrix (ECM), and its deposition is strictly related...