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A workflow for the development of template-assisted membrane crystallization downstream processing for monoclonal antibody purification
Monoclonal antibodies (mAbs) are commonly used biologic drugs for the treatment of diseases such as rheumatoid arthritis, multiple sclerosis,...
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Substitution of the communication-mediating domain and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens
Bacillomycin D is a nonribosomal peptide produced by Bacillus amyloliquefaciens fmbJ. In this study, the biosynthesis of bacillomycin D was mediated...
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Untargeted Soil Metabolomics Using Liquid Chromatography–Mass Spectrometry and Gas Chromatography–Mass Spectrometry
The molecular composition of soil organic matter (SOM) sets the foundation for terrestrial microbial community structures and carbon cycling... -
High-Resolution Lysine Acetylome Profiling by Offline Fractionation and Immunoprecipitation
Acetylation of lysine side chains at their ε-amino group is a reversible posttranslational modification (PTM), which can affect diverse protein... -
Assessing the Bioavailability and Testing the Effectiveness of Nutraceuticals and Nanonutraceuticals
In recent days, foods rich in nutraceuticals are in demand because of their nature to prevent or heal several chronic diseases. Efficiency of... -
Capillary Electrophoresis-Based N-Glycosylation Analysis in the Biomedical and Biopharmaceutical Fields
Glycomics has a growing interest in the biopharmaceutical industry and biomedical research requiring new high-performance and high-sensitivity... -
Seafood cross-contamination by tetrodotoxin (TTX): management of an unusual route of exposure
In this study, liquid chromatography with tandem mass spectroscopy was applied to detect Tetrodotoxin and analogues (TTXs) on pufferfish ( Sphoeroides...
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Integrating multiomics data accelerates elucidation of plant primary and secondary metabolic pathways
Plants are the most important sources of food for humans, as well as supplying many ingredients that are of great importance for human health....
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Plastisphere community assemblage of aquatic environment: plastic-microbe interaction, role in degradation and characterization technologies
It is undeniable that plastics are ubiquitous and a threat to global ecosystems. Plastic waste is transformed into microplastics (MPs) through...
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Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture – interaction studies based on a multi-OMICs approach
In their natural habitats, microbes rarely exist in isolation; instead, they thrive in consortia, where various interactions occur. In this study, a...
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Exploring the hypolipidemic effects of bergenin from Saxifraga melanocentra Franch: mechanistic insights and potential for hyperlipidemia treatment
ObjectiveThe goal of this study was to explore the hypolipidemic effects of bergenin extracted from Saxifraga melanocentra Franch ( S. melanocentra ),...
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Production of a food grade extract of Chlorococcum amblystomatis rich in omega-3 lipids using ethanol assisted with ultrasound and deep characterization by lipidomics
Chlorococcum amblystomatis is a novel microalga with a high content of omega-3 fatty acids (FAs), with applications for food, feed, nutraceuticals,...
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Purification and Identification of Bioactive Oligopeptide from Indian Halibut (Psettodes erumei) Muscle Tissue and Its Inflammation Suppressing Effect In Vitro
In this study, sole fish ( Psettodes erumei ) muscle mass was used for the isolation of inflammation suppressing peptide through the enzymatic...
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Analytical Platforms for Mass Spectrometry-Based Metabolomics of Polar and Ionizable Metabolites
Metabolomics studies rely on the availability of suitable analytical platforms to determine a vast collection of chemically diverse metabolites in... -
Common Methods of Extraction and Determination of Phytomelatonin in Plants
The presence of melatonin in plants, called phytomelatonin, has gained great interest in recent years. The determination of phytomelatonin levels in... -
Cryo-EM structure of human sphingomyelin synthase and its mechanistic implications for sphingomyelin synthesis
Sphingomyelin (SM) has key roles in modulating mammalian membrane properties and serves as an important pool for bioactive molecules. SM biosynthesis...
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Antibiofilm Activity of the Marine Probiotic Bacillus subtilis C3 Against the Aquaculture-Relevant Pathogen Vibrio harveyi
V. harveyi is a well-known pathogen-inducing vibriosis, especially for shrimp, fish, and invertebrates. Its virulence is related to biofilm formation...
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Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer
Ultra performance liquid chromatography-mass spectrometry (UPLC-MS) is the most frequently applied analytical platform in the untargeted metabolomic... -
Clinical advances in analytical profiling of signature lipids: implications for severe non-communicable and neurodegenerative diseases
BackgroundLipids play key roles in numerous biological processes, including energy storage, cell membrane structure, signaling, immune responses, and...
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Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry
Analysis and quantification of ether-lipid phospholipid species―also known as plasmalogens―is a crucial step in the study of the biological functions...