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Isolation and biochemical characterization of a novel serine protease identified from solid tannery waste metagenome
Metagenomics facilitates the isolation of novel enzymes from the unculturable microorganisms inhabiting a particular ecosystem. In the present study,...
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Characterization of an Intracellular Alkaline Serine Protease from Bacillus velezensis SW5 with Fibrinolytic Activity
ISP-SW5 is an intracellular alkaline serine protease gene from Bacillus velezensis SW5 that was heterologously expressed in Escherichia coli BL21...
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Purification and characterization of a new protease inhibitor from Spatholobus parviflorus seeds which inhibits the larval gut proteases of Spodoptera mauritia (Boisduval) (Lepidoptera: Noctuidae)
Plant protease inhibitors are defense molecules against pests, predators and pathogens. We purified and characterized a new 14 kDa protease inhibitor...
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Protease-Catalyzed Rational Synthesis of Uric Acid-Lowering Peptides in Non-aqueous Medium
Gout with etiological hyperuricemia deteriorates patient's quality of life, making an urgent need for effective uric acid-lowering drugs. Uric...
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Production of autolysis-proof Kex2 protease from Candida albicans in Saccharomyces cerevisiae for in vitro processing of fusion proteins
AbstractProtein expression with a fusion partner followed by the removal of the fusion partner via in vitro processing with a specific endoprotease...
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In Silico Study of Alkaline Serine Protease and Production Optimization in Bacillus sp. Khoz1 Closed Bacillus safensis Isolated from Honey
Protease is a kind of enzyme with different applications in medicine and industry. The limitation of the enzymes in industry is lack of tolerance for...
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Functional Differences of Digestive Proteases in Three Fish Species of the Genus Poblana (Atheriniformes: Atherinopsidae)
AbstractThe populations of three members of the Neotropical silverside fish from the Poblana genus of the Atherinopsidae family ( Poblana alchichica , P...
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Biosensor guided effective degradation of anti-nutritional factors via selected soybean fermentation process
Soybean meal (SM) is an important protein source for animal feed, and the β-conglycinin included is a significant allergen that can cause severe...
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Production of Alkaline Proteases using Aspergillus sp. Isolated from Injera: RSM-GA Based Process Optimization and Enzyme Kinetics Aspect
Alkaline proteases are well known to be significant industrial enzymes. This study focused on isolating the fungus producing proteases from, a...
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Alkaline protease for an efficacious rehydration of skin matrix by a novel Bacillus crolab MTCC 5468 in sustainable leather production: a green approach
ObjectivesThe utilization of biotechnology in leather sector has more extensive in modern years; more particular to proteolytic enzymes and employed...
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Catalytic esterification performance of protease in micro-aqueous system
ObjectiveTo evaluate the catalytic esterification performance of proteases in micro-aqueous systems and to study the suitable conditions for...
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A new perspective on the function of Tissue Non-Specific Alkaline Phosphatase: from bone mineralization to intra-cellular lipid accumulation
Tissue-nonspecific alkaline phosphatase (TNAP) is one of four isozymes, which include germ cell, placental and intestinal alkaline phosphatases. The...
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Expression of recombinant protease MarP from Mycobacterium tuberculosis in Pichia pastoris and its effect on human monocytes
ObjectiveMycobacterial acid-resistant protease (MarP) is a membrane-associated serine protease involved in the survival of Mycobacterium tuberculosis ...
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Effect of lon Protease Overexpression on Endotoxin Production and Stress Resistance in Bacillus thuringiensis
Lon protease, an intracellular protease, plays a key role in cell homeostasis in bacteria and is involved in numerous physiological processes. In...
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Plant Proteinase Inhibitor and Protease Interaction During Insect-Plant Communication
The interaction of proteinases with plant proteinase inhibitors (PPIs) has always been important in insect and plant interaction studies. Available...
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Characterization of a Protease Hyper-Productive Mutant of Bacillus pumilus by Comparative Genomic and Transcriptomic Analysis
Bacillus pumilus BA06 has great potential for the production of alkaline proteases. To improve the protease yield, classical mutagenesis to combine...
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Optimizing the acceleration of Cheddar cheese ripening using response surface methodology by microbial protease without altering its quality features
Cheddar cheese proteolysis were accelerated employing Penicillium candidum PCA1/TT031 protease into cheese curd. In the present study, several of the...
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A Novel Thermo-Alkaline Stable GDSL/SGNH Esterase with Broad Substrate Specificity from a Deep-Sea Pseudomonas sp.
Marine environments harbor a plethora of microorganisms that represent a valuable source of new biomolecules of biotechnological interest. In...
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A novel thermostable serine protease from a metagenomic library derived from marine sediments in the East China Sea
AbstractThermal activity and stability are important characteristics for proteases applied in the detergent, pharmaceutical, food, and other green...
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